CRISPR-Cas9 Gene Editing for Targeting Cancer Stem Cells in Glioblastoma Multiforme

Indus journal of bioscience research., Journal Year: 2025, Volume and Issue: 3(2), P. 394 - 407

Published: Feb. 28, 2025

This research investigates the possibility of CRISPR-Cas9 gene editing in targeting glioblastoma multiforme (GBM) cancer stem cells (CSCs) for increasing CSC sensitivity to conventional treatments and suppressing tumor growth. A quantitative method was used, with a sample 36 GBM patients diagnosed treated at major tertiary care centers Pakistan, namely Aga Khan University Hospital (Karachi), Shaukat Khanum Memorial Cancer (Lahore), Pakistan Institute Medical Sciences (Islamabad). Tumor tissue samples were obtained time surgical resection processed harvest CSCs based on certain markers like CD133 Nestin using fluorescence-activated cell sorting (FACS). The subsequently conducted isolated knock out genes interest involved stemness therapy resistance, such as SOX2, MGMT, Wnt/β-catenin. efficacy evaluated by pre- post-CRISPR growth rates, proliferation assays vitro, neurosphere formation. Multiple regression analysis showed that greatly enhanced (B = 1.427, p 0.000), pre-CRISPR rate -0.512, 0.009) initial size -0.312, 0.040) having negative correlation treatment. Moreover, increased MGMT expression 0.050) related decreased sensitivity. ANOVA test significant variability among efficacies different delivery methods CRISPR-Cas9, including viral vectors, nanoparticles, electroporation (F 4.56, 0.008), pointed towards optimizing strategies achieve efficient editing. shows promise treatment, but issues off-target effects need resolution enable future clinical applications.

Language: Английский

Long non-coding RNAs: a promising tool to improve horticultural quality traits

Current Plant Biology, Journal Year: 2024, Volume and Issue: 40, P. 100413 - 100413

Published: Nov. 6, 2024

Language: Английский