Suppression of amber stop codons impairs pathogenicity in Salmonella DOI Creative Commons
Zhihui Lyu,

Cierra Wilson,

Prajita Paul

et al.

FEBS Letters, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 12, 2024

Translation terminates at UAG (amber), UGA (opal), and UAA (ochre) stop codons. In nature, readthrough of codons can be substantially enhanced by suppressor tRNAs. Stop‐codon suppression also provides powerful tools in synthetic biology disease treatment. How stop‐codon affects bacterial pathogenesis is poorly understood. Here, we show that codons, but not or attenuates expression Salmonella Pathogenicity Island 1 (SPI‐1) genes, which are required for virulence. Consistently, amber abolishes infection macrophages. Systematic genetic biochemical analyses further decreases the activity, level, master SPI‐1 regulator HilD. Our work thus demonstrates an unexpected selectivity regulating

Language: Английский

Overcoming Challenges with Biochemical Studies of Selenocysteine and Selenoproteins DOI Open Access
A. J. Cain, Natalie Krahn

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(18), P. 10101 - 10101

Published: Sept. 20, 2024

Selenocysteine (Sec) is an essential amino acid that distinguishes itself from cysteine by a selenium atom in place of sulfur atom. This single change imparts distinct chemical properties to Sec which are crucial for selenoprotein (Sec-containing protein) function. These include lower pKa, enhanced nucleophilicity, and reversible oxidation. However, studying incorporation proteins complex process. While we find all domains life, each domain has translation mechanisms. mechanisms unique canonical composed Sec-specific enzymes mRNA hairpin drive recoding the UGA stop codon with Sec. In this review, highlight obstacles arise when investigating insertion, role proteins. We discuss strategic methods implemented field address these challenges. Though system complex, remarkable amount information been obtained specialized tools have developed. Continued studies area will provide deeper understanding on context proteins, necessity maintaining machinery make selenoproteins.

Language: Английский

Citations

1
From non-coding to coding: The importance of long non-coding RNA translation in de novo gene birth DOI
Taichi Shiraishi, Akinobu Matsumoto

Biochimica et Biophysica Acta (BBA) - General Subjects, Journal Year: 2024, Volume and Issue: unknown, P. 130747 - 130747

Published: Dec. 1, 2024

Language: Английский

Citations

1
Suppression of amber stop codons impairs pathogenicity in Salmonella DOI Creative Commons
Zhihui Lyu,

Cierra Wilson,

Prajita Paul

et al.

FEBS Letters, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 12, 2024

Translation terminates at UAG (amber), UGA (opal), and UAA (ochre) stop codons. In nature, readthrough of codons can be substantially enhanced by suppressor tRNAs. Stop‐codon suppression also provides powerful tools in synthetic biology disease treatment. How stop‐codon affects bacterial pathogenesis is poorly understood. Here, we show that codons, but not or attenuates expression Salmonella Pathogenicity Island 1 (SPI‐1) genes, which are required for virulence. Consistently, amber abolishes infection macrophages. Systematic genetic biochemical analyses further decreases the activity, level, master SPI‐1 regulator HilD. Our work thus demonstrates an unexpected selectivity regulating

Language: Английский

Citations

0