
Cells, Journal Year: 2025, Volume and Issue: 14(6), P. 412 - 412
Published: March 11, 2025
Cell cycle progression of the yeast Saccharomyces cerevisiae is largely driven by expression cyclins, which in turn bind cyclin-dependent kinase CDK1 providing specificity. Due to duplication genome during evolution, most cyclins are present as a pair paralogues, considered have similar functions and periods expression. Here, we use single molecule inexpensive fluorescence situ hybridization (smiFISH) measure five pairs paralogous genes relevant for cell (CLN1/CLN2, CLB5/CLB6, CLB3/CLB4, CLB1/CLB2 ACE2/SWI5) large number unsynchronized cells representing all phases. We systematically compare their patterns strengths. In addition, also analyze effect knockout one part each on other gene. order classify into specific phases, developed convolutional neural network (CNN). find that levels some cell-cycle related paralogues differ correlation, with CLN1 CLN2 showing strong correlation CLB3 CLB4 weakest correlation. The temporal profiles differ. Upon deletion paralogue, CLB1 CLB2 seem compensate gene, while this was not observed ACE2/SWI5. Interestingly, share work between mother bud G2 phase, where primarily expressed mother. Taken together, our results suggest should be same but both strength timing well precise role regulation.
Language: Английский