In Vitro Evaluation of the Safety and Antineoplastic Effects in Gastrointestinal Tumors of Nanostructured Lipid Carriers Loaded with Berberine DOI Creative Commons
Denitsa Stefanova, Yordan Yordanov,

Radostina Bogdanova

et al.

Pharmaceutics, Journal Year: 2025, Volume and Issue: 17(3), P. 331 - 331

Published: March 4, 2025

Background/Objectives: Natural substances have been a widely studied source of both pharmaceutical excipients and drugs. Berberine (BRB) is benzylisoquinoline alkaloid isolated from different plant sources. It possesses various pharmacological properties including antibacterial, antitumor, antidiabetic, neuroprotective, hepatoprotective, anti-inflammatory, antioxidant, etc. However, the limited aqueous solubility hinders its application. Nanosized drug delivery systems are an innovative approach for addressing challenges regarding via routes administration. Their utilization could improve active constituents. Methods: A melt-emulsification ultrasonication technique was applied preparation nanostructured lipid carriers (NLCs). They were thoroughly physicochemically characterized by means Dynamic Light Scattering, TEM, FTIR, DSC, TGA, In Vitro release. The efficacy safety evaluated on cholangiocarcinoma, colorectal adenocarcinoma, hepatocellular carcinoma, lymphoma, fibroblast, cardioblast cells, as well rat liver microsomes cytotoxicity assays comet assay. Results: obtained nanoparticles had spherical shape size around 158.2 ± 1.8 nm with negative zeta potential. revealed successful loading improved dissolution berberine in physiological conditions. studies showed that BRB NLCs resulted or retained to tumor cell lines reduced normal microsomes. NLC itself increased microsomal malondialdehyde (MDA) formation. Conclusions: presented. nanocarriers show favorable physicochemical biopharmaceutical properties. cellular experiments anticancer safety. These findings highlight potential applicability gastrointestinal neoplasms build foundation future practical translation.

Language: Английский

Demystifying the Potential of Polymeric Lipids as Substitute in Regenerative Applications: A Review DOI Open Access
Popat Mohite, Abhijeet Puri, Deepak Bharati

et al.

Polymers for Advanced Technologies, Journal Year: 2025, Volume and Issue: 36(2)

Published: Feb. 1, 2025

ABSTRACT The objective of a regenerative medicine is to repair, restore, and regenerate tissues organs that occurred due injury or defect disease. Additionally, the purpose reverse aging process body by utilizing body's innate healing ability. There still significant venture needs be directed for advancement in arena medicine, which makes use products derived from cell therapy as well biomedical. Stem cells growth factors are primary components medicine; hence, novel drug delivering systems now being studied augment transport active pharmaceuticals. Lipid‐based complexed with therapeutics suited site‐specific controlled release. Lipids biocompatible available various sources molecular weights self‐assemble into forms hydrophilic hydrophobic parts. By enhancing plasma membrane fluidity, excipients such non‐ionic surfactants, fatty acids, glycerides increase permeability. Liposomes, nanoparticles, nano‐emulsions, micelles nano‐lipid carriers can improve bioavailability both lipophilic medicines. lipoidal‐polymeric had emerged promising multifaceted application cytocompatibility, ecofriendly, ability encapsulate variety drugs. Further, treatment biomedical tissue engineering, rapidly developing discipline. Furthermore, similar vein, delivery technologies combined stem promote engraftment, differentiation, survival. Hence colloidal carrier has impending versatile applications. Therefore, review offer an abridged information on significance technology offering confined ecosystem facilitates them thrive distinguish effective manner.

Language: Английский

Citations

0

In Vitro Evaluation of the Safety and Antineoplastic Effects in Gastrointestinal Tumors of Nanostructured Lipid Carriers Loaded with Berberine DOI Creative Commons
Denitsa Stefanova, Yordan Yordanov,

Radostina Bogdanova

et al.

Pharmaceutics, Journal Year: 2025, Volume and Issue: 17(3), P. 331 - 331

Published: March 4, 2025

Background/Objectives: Natural substances have been a widely studied source of both pharmaceutical excipients and drugs. Berberine (BRB) is benzylisoquinoline alkaloid isolated from different plant sources. It possesses various pharmacological properties including antibacterial, antitumor, antidiabetic, neuroprotective, hepatoprotective, anti-inflammatory, antioxidant, etc. However, the limited aqueous solubility hinders its application. Nanosized drug delivery systems are an innovative approach for addressing challenges regarding via routes administration. Their utilization could improve active constituents. Methods: A melt-emulsification ultrasonication technique was applied preparation nanostructured lipid carriers (NLCs). They were thoroughly physicochemically characterized by means Dynamic Light Scattering, TEM, FTIR, DSC, TGA, In Vitro release. The efficacy safety evaluated on cholangiocarcinoma, colorectal adenocarcinoma, hepatocellular carcinoma, lymphoma, fibroblast, cardioblast cells, as well rat liver microsomes cytotoxicity assays comet assay. Results: obtained nanoparticles had spherical shape size around 158.2 ± 1.8 nm with negative zeta potential. revealed successful loading improved dissolution berberine in physiological conditions. studies showed that BRB NLCs resulted or retained to tumor cell lines reduced normal microsomes. NLC itself increased microsomal malondialdehyde (MDA) formation. Conclusions: presented. nanocarriers show favorable physicochemical biopharmaceutical properties. cellular experiments anticancer safety. These findings highlight potential applicability gastrointestinal neoplasms build foundation future practical translation.

Language: Английский

Citations

0