Normal butanol fraction of Polygonum hydropiper L. flavonoids reduces inflammation caused by PCV2 infections in cell and mouse models DOI Creative Commons

Yuheng Wei,

Shu‐Mian Zhou,

Wen Zhao

et al.

Frontiers in Veterinary Science, Journal Year: 2025, Volume and Issue: 12

Published: Feb. 4, 2025

Introduction The normal butanol fraction of Polygonum hydropiper L. flavonoids (FNB) exhibits significant anti-inflammatory effects. This study investigated FNB's impact on inflammatory responses induced by Porcine circovirus type 2 (PCV2) in cell and mouse models. Methods An model was established RAW264.7 cells infected with varying PCV2 concentrations. And assigning both 108 SPF-grade KM mice to Control, PCV2, Rutin, various dosages FNB groups. Inflammatory factors such as Monocyte Chemoattractant Protein-1 (MCP-1), interleukin-6 (IL-6), IL-8, IL-10, Tumor Necrosis Factor-alpha (TNF-α), Reactive Oxygen Species (ROS), Nitric Oxide (NO) were quantified using ELISA, RT-qPCR immunohistochemistry. Results showed that a titer 10 4.5 TCID 50 /0.1 mL when applied effectively an vitro at 12 24 h post-infection. Following infection, all the displayed increased culture supernatant intracellular mRNA expression levels ( p < 0.05 or 0.01), but these reduced treatment 0.01). In sera post-PCV2 infection also elevated IL-6, IL-8 TNF-α, MCP-1 Additionally, protein for iNOS rose significantly lung tissues 0.01) decreased Discussion These findings suggest reduces factor production modulates response triggered potentially enhancing host resistance against it.

Language: Английский

pH- and Matrix Metalloproteinase-Responsive Multifunctional Bilayer Microneedles Platform for Treatment of Tinea Pedis DOI Creative Commons

Musheng Yang,

Lingling Pan,

Hongmei Tian

et al.

ACS Biomaterials Science & Engineering, Journal Year: 2024, Volume and Issue: 10(5), P. 3108 - 3119

Published: April 25, 2024

Persistent foot odor and itchiness are common symptoms of tinea pedis, significantly disrupting the daily life those affected. The cuticular barrier at site pedis is thickened, which impedes effective penetration antifungal agents. Additionally, fungi can migrate from skin surface to deeper tissues, posing challenges in current clinical treatment for pedis. To effectively treat we developed a platform bilayer gelatin methacrylate (GelMA) microneedles (MNs) loaded with salicylic acid (SA) FK13-a1 (SA/FK13-a1@GelMA MNs). SA/FK13-a1@GelMA MNs exhibit pH- matrix metalloproteinase (MMP)-responsive properties efficient drug delivery. designed deliver deep into stratum corneum, softening cuticle creating microchannels. This process enables antibacterial peptide penetrate through corneum barrier, facilitating intradermal diffusion exerting anti-inflammatory effects. In severe cases heightened local pH levels MMP activity further accelerate release. Our research demonstrates that highly against Trichophyton mentagrophytes, rubrum, Candida albicans. They also reduced thickness, fungal burden, inflammation guinea pig model induced by T. mentagrophytes. Furthermore, it was discovered excellent biocompatibility. These findings suggest have significant potential as well other disorders.

Language: Английский

Citations

4
Tofacitinib Citrate Coordination‐Based Dual‐Responsive/Scavenge Nanoplatform Toward Regulate Colonic Inflammatory Microenvironment for Relieving Colitis DOI
Jiafeng Zou, Kun Jiang, You Chen

et al.

Advanced Healthcare Materials, Journal Year: 2024, Volume and Issue: 13(30)

Published: Aug. 23, 2024

Abstract Ulcerative colitis is an inflammation of the colon characterized by immune dysregulation and intestinal inflammation. Developing safe oral nanomedicines that suppress inflammation, while modulating colonic inflammatory microenvironment scavenging reactive oxygen species (ROS) hydrogen sulfide (H 2 S) crucial for effective treatment colitis. Here, tofacitinib citrate copper coordination‐based nanoparticle (TF‐Cu nanoparticle, T‐C) to dual‐scavenge ROS H S coordination competition synthesized. Moreover, T‐C using computer simulation explored. To enhance acid stability targeting T‐C, it encapsulated with hyaluronic acid‐modified chitosan, along a calcium pectinate coating (T‐C@HP). Owing dual pH/pectinase‐responsive characteristics T‐C@HP, nanoplatform can target inflamed lesions, inhibiting phosphorylated Janus kinase 1. Furthermore, T‐C@HP scavenges S, as well increases NADPH levels, which investigated combining biosensor (HyPer7 iNap1/c) chemical probes. also alleviates regulating through multiple processes, including modulation apoptosis, macrophage polarization, tight junction, mucus layer, flora. Complemented satisfactory anti‐inflammatory biosafety results, this represents promising, effective, option patients.

Language: Английский

Citations

4
In vitro immunotoxic evaluation of herbicides in RAW 264.7 cells DOI
Larissa Vivan Cestonaro, A Conte, Fernanda Capitânio Goldoni

et al.

Journal of Toxicology and Environmental Health, Journal Year: 2025, Volume and Issue: unknown, P. 1 - 14

Published: Jan. 15, 2025

Weeds are a concern in agriculture and the use of herbicides constitutes an effective, efficient, economical way to control their growth. Recent discoveries promising for management resistant weeds. However, there is gap knowledge toxic effects some previously reported on immune cells. The present study aimed examine cellular immunotoxicity three (clomazone, glyphosate, sulfentrazone) after 96 hr incubation utilizing RAW 264.7 BALB/c mouse monocyte/macrophage-like cell line elucidate role toxicological pathways. Data demonstrated clomazone, sulfentrazone initiated cytotoxic effect as evidenced by EC50 values 429.2; 53.7; 866.6 mg/L, respectively. Clomazone sulfentrazone, at all concentrations, induced excess production reactive oxygen (ROS) nitrogen (RNS) free radicals. An immunosuppression was observed cells with 50 or 100 mg/L glyphosate 500 1000 sulfentrazone. In addition, produced mitochondrial depolarization decreased tumor necrosis factor-α (TNF-α) levels. This first report clomazone cells, including reduced TNF-α levels, indicating adverse influence system.

Language: Английский

Citations

0
Biomimetic polydopamine nanoassembly regulates M2 macrophage polarization to alleviate radiation-induced pulmonary fibrosis via the PI3K/AKT/S100A4 pathway DOI
Li Shen, Shiyan Fu, Wenrun Li

et al.

Chemical Engineering Journal, Journal Year: 2025, Volume and Issue: unknown, P. 159980 - 159980

Published: Jan. 1, 2025

Language: Английский

Citations

0
Normal butanol fraction of Polygonum hydropiper L. flavonoids reduces inflammation caused by PCV2 infections in cell and mouse models DOI Creative Commons

Yuheng Wei,

Shu‐Mian Zhou,

Wen Zhao

et al.

Frontiers in Veterinary Science, Journal Year: 2025, Volume and Issue: 12

Published: Feb. 4, 2025

Introduction The normal butanol fraction of Polygonum hydropiper L. flavonoids (FNB) exhibits significant anti-inflammatory effects. This study investigated FNB's impact on inflammatory responses induced by Porcine circovirus type 2 (PCV2) in cell and mouse models. Methods An model was established RAW264.7 cells infected with varying PCV2 concentrations. And assigning both 108 SPF-grade KM mice to Control, PCV2, Rutin, various dosages FNB groups. Inflammatory factors such as Monocyte Chemoattractant Protein-1 (MCP-1), interleukin-6 (IL-6), IL-8, IL-10, Tumor Necrosis Factor-alpha (TNF-α), Reactive Oxygen Species (ROS), Nitric Oxide (NO) were quantified using ELISA, RT-qPCR immunohistochemistry. Results showed that a titer 10 4.5 TCID 50 /0.1 mL when applied effectively an vitro at 12 24 h post-infection. Following infection, all the displayed increased culture supernatant intracellular mRNA expression levels ( p < 0.05 or 0.01), but these reduced treatment 0.01). In sera post-PCV2 infection also elevated IL-6, IL-8 TNF-α, MCP-1 Additionally, protein for iNOS rose significantly lung tissues 0.01) decreased Discussion These findings suggest reduces factor production modulates response triggered potentially enhancing host resistance against it.

Language: Английский

Citations

0