Autoimmunity Reviews, Journal Year: 2024, Volume and Issue: 23(7-8), P. 103541 - 103541
Published: April 7, 2024
Language: Английский
Frontiers in Cellular and Infection Microbiology, Journal Year: 2023, Volume and Issue: 12
Published: Jan. 4, 2023
Objectives Recent studies have shown that fecal microbiota transplantation (FMT) improved the metabolic profiles of patients with type 2 diabetes mellitus (T2DM), yet effectiveness in reversing insulin resistance and increasing metformin sensitivity T2DM not been reported. In this study, we evaluated improvements their gut by FMT alone plus metformin. Methods A total 31 newly diagnosed were randomized to intervention metformin, FMT, or study. Patients followed up at baseline week 4 after treatment. Blood stool samples collected subject analyze clinical parameters microbial communities metagenomic sequencing, respectively. Results significantly indicators HOMA-IR BMI T2DM, besides fasting blood glucose, postprandial hemoglobin A1c also controlled Donor effectively colonized slightly higher colonization ration than within weeks, resulting increased diversity community changes from 227 species 441 alerted associated parameters. Among them, Chlorobium phaeovibrioides, Bifidibacterium adolescentis Synechococcus sp. WH8103 potential due negative correlations HOMA-IR. Conclusions without improve body mass index donor-derived microbiota.
Language: Английский
Citations
72
Food & Function, Journal Year: 2024, Volume and Issue: 15(10), P. 5596 - 5612
Published: Jan. 1, 2024
In the presented study, natural rice containing high resistant starch content was used as a raw material to produce (RRS) through enzymatic hydrolysis with heat-stable α-amylase and glucoamylase. The chemical composition, structural characteristics
Language: Английский
Citations
22
Nutrients, Journal Year: 2022, Volume and Issue: 14(14), P. 2993 - 2993
Published: July 21, 2022
Obesity is a complex chronic, relapsing, progressive disease. Association studies have linked microbiome alterations with obesity and overweight. However, the results are not always consistent. An integrated analysis of 4282 fecal samples (2236 control (normal weight) group, 1152 overweight, 894 simple obesity) was performed to identify obesity-associated microbial markers. Based on random effects model fixed model, we calculated odds ratios metrics, including bacterial alpha-diversity, beta-diversity, Bacteroidetes/Firmicutes ratio, common genera, pathways, between groups as well overweight groups. The forest trained based single dataset at genus level. Feature selection feature importance ranked by mean decrease accuracy leave-one-out cross-validation conducted improve predictive performance models. Chao1 evenness possessed significant ORs higher than 1.0 Significant community differences were observed control. ratio significantly in patients. relative abundance Lachnoclostridium Faecalitalea people obesity, while 23 Christensenellaceae_R-7_group, Akkermansia, Alistipes, Butyricimonas, etc., lower. achieved high (AUC = 0.83). adenine adenosine salvage pathway (PWY-6609) L-histidine degradation I (HISDEG-PWY) clustered obese patients, amino acid biosynthesis pathways (HISDEG-PWY, DAPLYSINESYN-PWY) decreased. This study identified biomarkers, providing fertile targets for management obesity.
Language: Английский
Citations
46
International Journal of Biological Sciences, Journal Year: 2022, Volume and Issue: 18(10), P. 3981 - 3992
Published: Jan. 1, 2022
Intestinal ischemia/reperfusion (I/R) is a common pathophysiological process in clinical severe patients, and the effect of intestinal I/R injury on patient's systemic state far greater than that primary injury.In recent years, more evidence has shown microbiota its metabolites play an important role occurrence, development, diagnosis treatment injury.Intestinal regulated by host genes, immune response, diet, drugs other factors.The metabolism potential determine significance health diseases.Therefore, targeting may be effective therapy for I/R-induced extraintestinal organ injury.This review focuses injury, summarizes latest progress regulating to treat injury.
Language: Английский
Citations
39
European Journal of Neurology, Journal Year: 2023, Volume and Issue: 30(11), P. 3581 - 3594
Published: Jan. 3, 2023
The role of the gut microbiome in pathogenesis Parkinson disease (PD) is under intense investigation, and results presented are still very heterogeneous. These discrepancies arise not only from highly heterogeneous pathology PD, but also widely varying methodologies at all stages workflow, sampling to final statistical analysis. aim present work harmonize workflow across studies reduce methodological heterogeneity perform a pooled analysis account for other sources heterogeneity.We performed systematic review identify comparing microbiota PD patients healthy controls. A was designed processing bioinformatics using Bayesian random-effects meta-analysis based on individual patient-level data.The show that harmonizing workflows minimizes differences between methods reveals small set taxa being associated with PD. Increased shares genera Akkermansia Bifidobacterium decreased Roseburia Faecalibacterium were most characteristic PD-associated microbiota.Our study summarizes evidence reduced levels butyrate-producing combination possible degradation mucus layer by may promote intestinal inflammation permeability mucosal layer. This allow potentially pathogenic metabolites transit enter enteric nervous system.
Language: Английский
Citations
34
mSystems, Journal Year: 2023, Volume and Issue: 8(1)
Published: Jan. 23, 2023
Metformin is a common drug for the management of type 2 diabetes mellitus; however, it causes various adverse gastrointestinal effects, especially after prolonged treatment. It thus interest to identify an adjuvant treatment that synergizes with efficacy metformin while mitigating its effects. Since previous evidence supports gut microbiota target metformin, this study investigated beneficial effect and mechanism coadministration probiotics in mellitus by conducting 3-month randomized, double-blind, placebo-controlled clinical trial (n = 27 21 probiotic placebo groups, respectively, who completed trial). Clinical results showed significantly reduced glycated hemoglobin compared taken alone (P < 0.05). Metagenomic metabolomic analyses increased abundance short-chain fatty acid (SCFA)-producing bacteria bile acids. Significantly or marginally more acids related metabolites were detected group than postintervention. Taken together, our synergized hypoglycemic patients mellitus, which was likely through modulating microbiome and, subsequently, SCFA metabolism. Our findings support cotreatment mellitus. IMPORTANCE variable We found Probio-X may promote SCFA-producing levels specific acids, increasing secretion hormones ultimately improving glucose homeostasis.
Language: Английский
Citations
23
Journal of Ethnopharmacology, Journal Year: 2023, Volume and Issue: 310, P. 116385 - 116385
Published: March 15, 2023
Propolis is a traditional natural medicine with various activities such as antioxidant and anti-inflammatory, immunomodulatory, anti-tumour, gastroenteritis treatment prevention, anti-microbial parasitic, well glucose regulation anti-diabetes, expected to be an anti-diabetic candidate few side effects, but the mechanism of action propolis on type 2 diabetes mellitus (T2DM) has not been fully elucidated.The purpose this study was investigate effect ethanol extract (EEP) blood in T2DM mice.We studied possible EEP using animal model induced by combination high-fat diet intraperitoneal injection streptozotocin (STZ). The experiment divided into four groups, namely, normal group (HC), (T2DM), metformin (MET). Biochemical indexes cytokines were measured, differences metabolites serum compared 1H-NMR. In addition, diversity intestinal flora feces 16S rDNA amplicon sequencing.The results showed that following MET, weight-loss trend mice alleviated, fasting glucose, insulin secretion level, resistance index, C peptide level oral tolerance decreased, whereas sensitivity index increased, thereby effectively alleviated occurrence resistance. Compared group, concentrations pro-inflammatory interleukin-1 beta (IL-1β), interleukin-6 (IL-6) tumor necrosis factor-alpha (TNF-α) decreased significantly MET anti-inflammatory cytokine interleukin-10 (IL-10) increased significantly. Metabolomics revealed regulate carbohydrate metabolism restore amino acid lipid metabolism. Correlation analysis mouse HC harmful bacteria Bilophila, Eubacterium_ventriosum_group, Mucispirillum Desulfovibrio found abundance beneficial Lactobacillus reduced. Parabacteroides, Akkermansia, Leuconostoc, Alloprevotella abundantly present group; however, increase genus Parasutterella, which could energy sensitivity.The reduce mice, followed alleviating resistance, improving inflammatory reaction regulating affecting steady state gut microbiota. However, overall therapeutic better than MET.
Language: Английский
Citations
23
BMC Medicine, Journal Year: 2023, Volume and Issue: 21(1)
Published: May 5, 2023
Abstract Background There is insufficient evidence for the ability of vitamin K2 to improve type 2 diabetes mellitus symptoms by regulating gut microbial composition. Herein, we aimed demonstrate key role microbiota in improvement impaired glycemic homeostasis and insulin sensitivity intervention. Methods We first performed a 6-month RCT on 60 T2DM participants with or without MK-7 (a natural form K2) In addition, conducted transplantation MK-7-regulated diet-induced obesity mice 4 weeks. 16S rRNA sequencing, fecal metabolomics, transcriptomics both study phases were used clarify potential mechanism. Results After intervention, observed notable 13.4%, 28.3%, 7.4% reductions fasting serum glucose ( P = 0.048), 0.005), HbA1c levels 0.019) significant tolerance 0.005). Moreover, increased concentrations secondary bile acids (lithocholic taurodeoxycholic acid) short-chain fatty (acetic acid, butyric valeric found human mouse feces accompanied an abundance genera that are responsible biosynthesis these metabolites. Finally, weeks significantly improved activating colon acid receptors, improving host immune-inflammatory responses, increasing circulating GLP-1 concentrations. Conclusions Our gut-derived findings provide regulatory homeostasis, which may further facilitate clinical implementation intervention management. Trial registration The was registered at https://www.chictr.org.cn (ChiCTR1800019663).
Language: Английский
Citations
23
Metabolites, Journal Year: 2025, Volume and Issue: 15(1), P. 22 - 22
Published: Jan. 6, 2025
Background/Objectives: Managing type 2 diabetes mellitus (T2DM) and obesity requires a multidimensional, patient-centered approach including nutritional interventions (NIs) physical activity. Changes in the gut microbiota (GM) have been linked to metabolic alterations typical of T2DM obesity, they are strongly influenced by diet. However, few studies evaluated effects on GM very-low-calorie ketogenic diet (VLCKD) patients with T2DM, especially mid-term long-term. This longitudinal study is aimed at evaluating long-term impact VLCKD Mediterranean (MD) anthropometric, metabolic, lifestyle parameters 11 (diabesity). extends previously published results short-term (three months) these NIs same patients. Methods: At baseline, were randomly assigned either (KETO group) or (MEDI group). After two months, KETO group gradually shifted (VLCKD-MD), according current guidelines. From fourth month until end both groups followed similar MD. Previous showed that had more beneficial than MD several variables for 3 months NI. In this study, analyses extended six (T6) twelve (T12) NI comparing data prospectively against baseline (T0). The analysis was performed through next-generation sequencing. Results: Improvements anthropometric pronounced T6, particularly body mass index (−5.8 vs. −1.7 kg/m2; p = 0.006) waist circumference (−15.9 −5.2 cm; 0.011). significant improvement HbA1c (6.7% 5.5% 0.02) triglyceride (158 95 mg/dL 0.04) values compared T0 observed only group, which maintained achieved T3. VLCKD-MD phenotype. A substantial positive modulatory effect up sixth due progressive increase bacterial markers human health. month, most health decreased, though still increased baseline. Among them, Verrucomicrobiota phylum identified as main biomarker together its members Verrucomicrobiae, Akkermansiaceae, Verrucomicrobiales, Akkermansia T6 Conclusions: Both dietary approaches ameliorated status, but VLCKD, support MD, has shown greater improvements parameters, well profile,
Language: Английский
Citations
1
Metabolites, Journal Year: 2025, Volume and Issue: 15(2), P. 127 - 127
Published: Feb. 13, 2025
Numerous recent studies have suggested that the composition of intestinal microbiota can trigger metabolic disorders, such as diabetes, prediabetes, obesity, syndrome, sarcopenia, dyslipidemia, hyperhomocysteinemia, and non-alcoholic fatty liver disease. Since then, considerable effort has been made to understand link between well role probiotics in modulation microbiota. The aim this review was summarize reviews individual articles on state art regarding ideal therapy with prebiotics order obtain reversion dysbiosis (alteration microbiota) eubiosis during diseases, diseases. This includes 245 eligible studies. In conclusion, a condition dysbiosis, or general, alteration microbiota, could be implicated development disorders through different mechanisms, mainly linked release pro-inflammatory factors. Several already demonstrated potential using treatment condition, detecting significant improvements specific symptoms These findings reinforce hypothesis lead generalized inflammatory picture negative consequences organs systems. Moreover, confirms beneficial effects diseases are promising, but more research is needed determine optimal probiotic strains, doses, administration forms for conditions.
Language: Английский
Citations
1