Continuous, Label-Free Phenotyping of Single Cells Based on Antibody Interaction Profiling in Microfluidic Channels DOI
Thijs Roebroek, W. Van Roy,

Sophie Roth

et al.

Analytical Chemistry, Journal Year: 2025, Volume and Issue: unknown

Published: April 12, 2025

Flow cytometry commonly utilizes fluorescence labeling and extensive sample preparation to detect specific cell surface markers, making analysis under native conditions impractical. In this work, a label-free flow technique is presented that spatiotemporally resolves cell-surface interactions in antibody-coated microfluidic channels. Using computational imaging, numerous cells are tracked across large field of view (12 × 3 mm2) the resulting motion profiles used for phenotypic characterization. As proof-of-principle, experiments targeting T-cell receptor CD8 performed directly on cultures. Individual T-cells successfully 98% cases velocities 1-3 mm·s-1. 14 μm high channels coated with only nonspecific antibodies, both CD8-positive SUP-T1 CD8-negative Jurkat exhibit mostly constant velocities. contrast, using functionalized CD8-specific but not show temporary delays linked interaction. Cell classification based observed results clear contrast ratio 23.9 ± 11.6 (mean standard deviation) between at 1 The decreases higher as fewer interact due increased hydrodynamic lift. Our affirm our method's ability differentiate without prior or preparation.

Language: Английский

Histone demethylases in the regulation of immunity and inflammation DOI Creative Commons
Lihua Qu, Tong Yin,

Yijin Zhao

et al.

Cell Death Discovery, Journal Year: 2023, Volume and Issue: 9(1)

Published: June 23, 2023

Abstract Pathogens or danger signals trigger the immune response. Moderate response activation removes pathogens and avoids excessive inflammation tissue damage. Histone demethylases (KDMs) regulate gene expression play essential roles in numerous physiological processes by removing methyl groups from lysine residues on target proteins. Abnormal of KDMs is closely associated with pathogenesis various inflammatory diseases such as liver fibrosis, lung injury, autoimmune diseases. Despite becoming exciting targets for diagnosing treating these diseases, role enzymes regulation still unclear. Here, we review underlying mechanisms through which immune-related pathways responses. In addition, also discuss future applications inhibitors

Language: Английский

Citations

31
New cell sources for CAR-based immunotherapy DOI Creative Commons
Marzieh Mazinani, Fatemeh Rahbarizadeh

Biomarker Research, Journal Year: 2023, Volume and Issue: 11(1)

Published: May 6, 2023

Chimeric antigen receptor (CAR) T cell therapy, in which a patient's own lymphocytes are engineered to recognize and kill cancer cells, has achieved striking success some hematological malignancies preclinical clinical trials, resulting six FDA-approved CAR-T products currently available the market. Despite impressive outcomes, concerns about treatment failure associated with low efficacy or high cytotoxicity of cells remain. While main focus been on improving exploring alternative cellular sources for CAR generation garnered growing interest. In current review, we comprehensively evaluated other rather than conventional generation.

Language: Английский

Citations

27
Neutrophil Nanodecoys Inhibit Tumor Metastasis by Blocking the Interaction between Tumor Cells and Neutrophils DOI
Weiya Zeng, Yanru Wang, Qing Zhang

et al.

ACS Nano, Journal Year: 2024, Volume and Issue: 18(10), P. 7363 - 7378

Published: Feb. 29, 2024

Cancer metastasis is the main cause of cancer-related deaths and involves interaction between tumor cells neutrophils. In this study, we developed activated neutrophil membrane-coated nanoparticles (aNEM NPs) as nanodecoys to block neutrophil-mediated cancer metastasis. The aNEM NPs were fabricated by cloaking poly(lactic acid) with membranes derived from neutrophils inherited functional proteins We demonstrated that could interfere recruitment primary premetastatic niches, inhibit adhesion vascular endothelium circulating (CTCs), disrupt formation CTC-neutrophil clusters in vitro vivo. 4T1-bearing mice, effectively reduce breast various organs mice. Our results suggest are a promising nanomedicine for preventing or treating acting nanodecoys.

Language: Английский

Citations

15
Neutrophil diversity and function in health and disease DOI Creative Commons

F Zhang,

Yidan Xia,

Jiayang Su

et al.

Signal Transduction and Targeted Therapy, Journal Year: 2024, Volume and Issue: 9(1)

Published: Dec. 5, 2024

Abstract Neutrophils, the most abundant type of granulocyte, are widely recognized as one pivotal contributors to acute inflammatory response. Initially, neutrophils were considered mobile infantry innate immune system, tasked with immediate response invading pathogens. However, recent studies have demonstrated that versatile cells, capable regulating various biological processes and impacting both human health disease. Cytokines other active mediators regulate functional activity by activating multiple receptors on these thereby initiating downstream signal transduction pathways. Dysfunctions in disruptions neutrophil homeostasis been implicated pathogenesis numerous diseases, including cancer disorders, often due aberrant intracellular signaling. This review provides a comprehensive synthesis functions, integrating advancements this field. Moreover, it examines roles signaling pathways involved regulation activity. The pathophysiology diseases emerging therapeutic approaches targeting them also elaborated. addresses current limitations within field research, highlighting critical gaps knowledge warrant further investigation. In summary, seeks establish multidimensional model regulation, providing new perspectives for potential clinical applications research.

Language: Английский

Citations

11
Alpha-linolenic acid pretreatment alleviates NETs-induced alveolar macrophage pyroptosis by inhibiting pyrin inflammasome activation in a mouse model of sepsis-induced ALI/ARDS DOI Creative Commons
Chenchen Liu, Yu Zhou, Qing Tu

et al.

Frontiers in Immunology, Journal Year: 2023, Volume and Issue: 14

Published: March 27, 2023

Background Neutrophil extracellular traps (NETs) can cause acute lung injury (ALI)/acute respiratory distress syndrome (ARDS) by inducing macrophage pyroptosis. The purpose of this study was to find out whether pretreatment alpha-linolenic acid (ALA) could inhibit NETs-induced pyroptosis in sepsis-induced ALI/ARDS, as well identify which inflammasome is involved process. Methods LPS instilled into the trachea establish ALI/ARDS a mouse model. ​Lung assessed microscopic examination tissue after hematoxylin and eosin staining, pathology score, bronchoalveolar lavage fluid (BALF) total protein concentration. level NETs detected MPO-DNA ELISA. Purified NETs, extracted from peritoneal neutrophils, induced vitro . Expression pyroptosis-related proteins (Cl-caspase-1, Cl-GSDMD, ASC) IL-1β bone marrow-derived macrophages (BMDMs) were determined western blotting or Specks Pyrin/ASC examined confocal immunofluorescence microscopy. Mefv (Pyrin) -/- mice used role Pyrin process ALI/ARDS. Results ALA alleviated LPS-induced injury. reduced ASC), sepsis mice. In , increased expression significantly BMDMs. found be higher form with ASC challenged-BMDMs. Knockout gene fully restored vivo Lung (Pyrin)-/- well.​ suppresses all changes mentioned above. Conclusion Our first demonstrate driving pyroptosis, may reduce inhibiting activation inflammasome-driven

Language: Английский

Citations

23
The role of immune system in atherosclerosis: Molecular mechanisms, controversies, and future possibilities DOI
Tianle Zhang,

Chenxu Pang,

Mengxin Xu

et al.

Human Immunology, Journal Year: 2024, Volume and Issue: 85(2), P. 110765 - 110765

Published: Feb. 18, 2024

Language: Английский

Citations

9
Targeting CXCR2 signaling in inflammatory lung diseases: neutrophil-driven inflammation and emerging therapies DOI
Md Sadique Hussain, Ahsas Goyal,

Kavita Goyal

et al.

Naunyn-Schmiedeberg s Archives of Pharmacology, Journal Year: 2025, Volume and Issue: unknown

Published: March 6, 2025

Language: Английский

Citations

1
The Role of KH-Type Splicing Regulatory Protein (KSRP) for Immune Functions and Tumorigenesis DOI Creative Commons

Kim-Alicia Palzer,

Vanessa Bolduan,

Rudolf Käfer

et al.

Cells, Journal Year: 2022, Volume and Issue: 11(9), P. 1482 - 1482

Published: April 28, 2022

Post-transcriptional control of gene expression is one important mechanism that enables stringent and rapid modulation cytokine, chemokines or growth factors expression, all relevant for immune tumor cell function communication. The RNA-binding protein KH-type splicing regulatory (KSRP) controls the mRNA stability according genes by initiation decay inhibition translation, enhancing maturation microRNAs. Therefore, KSRP plays a pivotal role in progression. In this review, we summarize current knowledge about with regard to regulation immunologically targets, functional on responses tumorigenesis. involved myeloid hematopoiesis. Further, KSRP-mediated pro-inflammatory necessary keep homeostasis. case infection, impairment induction robust responses. regard, seems primarily dampen T helper 2 cancer, has often been associated metastasis. summary, aside decay, microRNA be especially its diverse biological functions, which warrants further in-depth examination.

Language: Английский

Citations

27
The prognostic utility of preoperative neutrophil-to-lymphocyte ratio (NLR) in patients with colorectal liver metastasis: a systematic review and meta-analysis DOI Creative Commons
Yanqing Li, Tianxiang Xu, Xin Wang

et al.

Cancer Cell International, Journal Year: 2023, Volume and Issue: 23(1)

Published: Feb. 28, 2023

Abstract The neutrophil-to-lymphocyte ratio is used to reflect body's inflammatory status with prognostic value in different cancers. We aimed investigate the influence of preoperative NLR prognosis CRLM patients receiving surgery using meta-analysis. Data Cochrane Library, PubMed, Embase, and Web Science databases created before October 2022 were recruited. Meta-analysis was carried out RevMan 5.3 Stata16 software, primary outcome indicators included overall survival (OS), secondary disease-free (DFS) relapse-free (RFS). pooled risk (HR) 95% confidence interval (CI) for each indicator determined random-effects models or fixed-effects models. odds (OR) corresponding intervals clinicopathological characteristics a model. 18 papers published between 2008 (3184 total) included. analysis found that high correlated poor OS (multivariate HR = 1.83, CI 1.61–2.08, p < 0.01), DFS 1.78, 1.16–2.71, 0.01) RFS 1.46, 1.15–1.85, but not related features correlation. In conclusion, an independent factor CRLM. More large-scale clinical researches are required future demonstrate inclusion as guide postoperative adjuvant chemotherapy.

Language: Английский

Citations

17
Ficolin-A/2 Aggravates Severe Lung Injury through Neutrophil Extracellular Traps Mediated by Gasdermin D–Induced Pyroptosis DOI

Li Huang,

Xiaowu Tan,

Weixia Xuan

et al.

American Journal Of Pathology, Journal Year: 2024, Volume and Issue: 194(6), P. 989 - 1006

Published: March 3, 2024

Language: Английский

Citations

6