Traditional Chinese medicine in regulating macrophage polarization in immune response of inflammatory diseases

Journal of Ethnopharmacology, Journal Year: 2024, Volume and Issue: 325, P. 117838 - 117838

Published: Feb. 3, 2024

Language: Английский

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Scutellarin alleviates renal ischemia–reperfusion injury by inhibiting the MAPK pathway and pro‐inflammatory macrophage polarization

The FASEB Journal, Journal Year: 2024, Volume and Issue: 38(13)

Published: July 3, 2024

Abstract Renal ischemia–reperfusion injury (IRI) is an integral process in renal transplantation, which results compromised graft survival. Macrophages play important role both the early inflammatory period and late fibrotic response to IRI. In this study, we investigated whether scutellarin (SCU) could protect against IRI by regulating macrophage polarization. Mice were given SCU (5–50 mg/kg) gavage 1 h earlier, followed a unilateral function pathological assessed 24 after reperfusion. The showed that administration of 50 mg/kg significantly improved pathology mice. addition, alleviated IRI‐induced apoptosis. Meanwhile, it reduced infiltration inhibited pro‐inflammatory Moreover, RAW 264.7 cells primary bone marrow‐derived macrophages (BMDMs) exposed SCU, found 150 μM these polarize phenotype induced lipopolysaccharide (LPS) interferon‐γ (IFN‐γ). However, has no influence on anti‐inflammatory polarization vivo vitro interleukin‐4 (IL‐4). Finally, explored effect activation mitogen‐activated protein kinase (MAPK) pathway vitro. We suppressed MAPK pathway, including extracellular signal‐regulated (ERK), Jun N‐terminal (JNK), p38. Our demonstrated protects kidney inhibiting toward via suggesting may be therapeutically treatment

Language: Английский

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Tannic acid‑cerium nanoenzymes serve as broad-spectrum antioxidants to alleviate acute kidney injury by modulating macrophage polarization, mitophagy and endoplasmic reticulum stress

Journal of Controlled Release, Journal Year: 2025, Volume and Issue: 380, P. 892 - 909

Published: Feb. 20, 2025

Language: Английский

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A General and Efficient Strategy for Gene Delivery Based on Tea Polyphenols Intercalation and Self‐Polymerization

Advanced Science, Journal Year: 2023, Volume and Issue: 10(24)

Published: June 22, 2023

Gene therapy that employs therapeutic nucleic acids to modulate gene expression has shown great promise for diseases therapy, and its clinical application relies on the development of effective vector. Herein a novel delivery strategy by just using natural polyphenol (-)-epigallocatechin-3-O-gallate (EGCG) as raw material is reported. EGCG first intercalates into yield complex, which then oxidizes self-polymerizes form tea polyphenols nanoparticles (TPNs) encapsulation. This general method load any types with single or double strands short long sequences. Such TPNs-based vector achieves comparable loading capacity commonly used cationic materials, but showing lower cytotoxicity. TPNs can effectively penetrate inside cells, escape from endo/lysosomes, release in response intracellular glutathione exert biological functions. To demonstrate vivo application, an anti-caspase-3 small interfering ribonucleic acid loaded treat concanavalin A-induced acute hepatitis, excellent efficacy obtained combination intrinsic activities work provides simple, versatile, cost-effective strategy. Given biocompatibility biofunctions, this holds potential various diseases.

Language: Английский

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The Role of Interferon Regulatory Factors in Liver Diseases

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(13), P. 6874 - 6874

Published: June 22, 2024

The interferon regulatory factors (IRFs) family comprises 11 members that are involved in various biological processes such as antiviral defense, cell proliferation regulation, differentiation, and apoptosis. Recent studies have highlighted the roles of IRF1-9 a range liver diseases, including hepatic ischemia-reperfusion injury (IRI), alcohol-induced injury, Con A-induced nonalcoholic fatty disease (NAFLD), cirrhosis, hepatocellular carcinoma (HCC). IRF1 is progression IRI through signaling pathways PIAS1/NFATc1/HDAC1/IRF1/p38 MAPK IRF1/JNK. regulation downstream IL-12, IL-15, p21, p38, HMGB1, JNK, Beclin1, β-catenin, caspase 3, 8, IFN-γ, IFN-β other genes IRI, development HCC PD-L1, IL-6, IL-8, CXCL1, CXCL10, CXCR3. In addition, IRF3-PPP2R1B IRF4-FSTL1-DIP2A/CD14 NAFLD. Other IRF also play moderately important functions different diseases. Therefore, given significance IRFs diseases lack comprehensive compilation their molecular mechanisms this review dedicated to exploring

Language: Английский

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Cornuside improves murine autoimmune hepatitis through inhibition of inflammatory responses

Phytomedicine, Journal Year: 2023, Volume and Issue: 120, P. 155077 - 155077

Published: Sept. 10, 2023

Autoimmune hepatitis (AIH) poses an important public health concern worldwide, with few therapeutic options available. Cornuside, a primary cornel iridoid glycoside present in Cornus officinalis Sieb. et Zucc., is well-known traditional Chinese medicine that possesses anti-inflammatory, antioxidant and anti-apoptotic properties. However, the effects of cornuside on autoimmune diseases including AIH still not defined, neither clear mechanisms suppression inflammatory responses.The study was aimed to investigate using murine models.A model induced by concanavalin A (Con A) used examine pharmacological activity suppressing responses vivo.C57BL/6J mice were intravenously different doses challenged 18 mg/kg Con 3 h later. Network analysis performed identify potential target genes signaling pathways AIH. Next serum liver tissues collected 12 after injection analyze levels markers for hepatic injury, apoptosis, oxidative stress, immune responses, inflammation.Network revealed may modulate stress apoptosis Compared group, pretreatment significantly reduced alanine aminotransferase aspartate aminotransferase, improving histopathological damage livers. In addition, decreased malondialdehyde, myeloperoxidase, but increased superoxide dismutase levels, suggesting relieving stress. Furthermore, suppressed activation T natural killer cells, whereas proportion myeloid-derived suppressor cells increased. The production proinflammatory cytokines, interleukin (IL)-6, IL-12, IL-1β, tumor necrosis factor-alpha (TNF-α), also clearly decreased. Finally, western blot displayed inhibited phosphorylation extracellular receptor kinase (ERK) c-Jun N-terminal (JNK).We demonstrated has protective A-induced immune-mediated through ERK JNK pathways, as well modulating recruitment cells.

Language: Английский

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Interleukin 28A aggravates Con A‐induced acute liver injury by promoting the recruitment of M1 macrophages

The FASEB Journal, Journal Year: 2024, Volume and Issue: 38(2)

Published: Jan. 24, 2024

Abstract Immune‐mediated acute hepatic injury is characterized by the destruction of a large number hepatocytes and severe liver function damage. Interleukin‐28A (IL‐28A), member IL‐10 family, notable for its antiviral properties. However, despite advances in our understanding IL‐28A, role immune‐mediated remains unclear. The present study investigated IL‐28A concanavalin A (Con A)‐induced immune injury. After Con injection mice, level significantly increased. deficiency was found to protect mice from injury, prolong survival time, reduce serum aspartate aminotransferase alanine levels. In contrast, recombinant aggravated mice. proportion activated M1 macrophages lower IL‐28A‐deficiency group than wild‐type mouse group. adoptive transfer experiments, WT could exacerbate symptoms Furthermore, expression proinflammatory cytokines, including tumor necrosis factor‐α (TNF‐α), IL‐12, IL‐6, IL‐1β, decreased Western blotting demonstrated that limit macrophage polarization modulating nuclear factor (NF)‐κB, mitogen‐activated protein kinase (MAPK), interferon regulatory (IRF) signaling pathways. summary, deletion plays an important protective A‐induced model inhibits activation inhibiting NF‐κB, MAPK, IRF These results provide potential new target treatment immune‐related

Language: Английский

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Sappanone A Ameliorates Concanavalin A-induced Immune-Mediated Liver Injury by Regulating M1 Macrophage Polarization

Inflammation, Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 26, 2024

Sappanone A (SAP), a high-isoflavone compound derived from the traditional Chinese medicine Sumu, exhibits various pharmacological activities, including anti-inflammatory and anti-oxidant effects. However, its protective effects on liver have rarely been reported. The aim of this study was to investigate SAP immune-mediated injury induced by concanavalin (Con A) in mice explore underlying molecular mechanisms. Mice were administered intraperitoneally (50 mg/kg body weight). Three hours later, Con (18 mg/kg) injected via tail vein induce damage. Livers blood collected 12 h after challenge. Liver cell apoptosis, oxidative stress, M1 macrophage activation vivo investigated. Bone marrow-derived macrophages used confirm polarization vitro. results indicated that decreased transaminase levels, inhibited improved stress mouse livers. Furthermore, significantly reduced proportion macrophages, expression CD86, downregulated macrophage-related inflammatory cytokines. Moreover, SAP-treated alleviated damage caused compared non-SAP-treated macrophages. Mechanistically, phosphorylation key molecules MAPK NF-κB signaling pathways resulting an inhibitory effect activation. Taken together, alleviates suppressing polarization, which is partially associated with pathways.

Language: Английский

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Cucurbitacin IIb mitigates concanavalin A-induced acute liver injury by suppressing M1 macrophage polarization

International Immunopharmacology, Journal Year: 2025, Volume and Issue: 147, P. 113964 - 113964

Published: Jan. 5, 2025

Language: Английский

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In Vivo and In Vitro Models of Hepatic Fibrosis for Pharmacodynamic Evaluation and Pathology Exploration

International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(2), P. 696 - 696

Published: Jan. 15, 2025

Hepatic fibrosis (HF) is an important pathological state in the progression of chronic liver disease to end-stage and usually triggered by alcohol, nonalcoholic fatty liver, hepatitis viruses, autoimmune (AIH), or cholestatic disease. Research on novel therapies has become a hot topic due reversibility HF. into molecular mechanisms pathology HF potential drug screening relies reliable rational biological models, mainly including animals cells. Hence, number modeling approaches have been attempted based human dietary, pathological, physiological factors development In this review, classical methods last 10 years were collected from electronic databases, Web Science, PubMed, ScienceDirect, ResearchGate, Baidu Scholar, CNKI. Animal models are induced chemical toxicants, special diets, pathogenic microorganisms, surgical operations, gene editing. The advantages limitations hepatic stellate cells (HSCs), organoids, 3D coculture-based established vitro also proposed summarized. This information provides scientific basis for discovery mechanism treatment

Language: Английский

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