Construction and Mechanism Exploration of Highly Efficient System for Bacterial Ghosts Preparation Based on Engineered Phage ID52 Lysis Protein E DOI Creative Commons
Yi Ma, Sijia Wang, Bin Hong

et al.

Vaccines, Journal Year: 2024, Volume and Issue: 12(5), P. 472 - 472

Published: April 28, 2024

Bacterial ghosts (BGs) are hollow bacterial cell envelopes with intact cellular structures, presenting as promising candidates for various biotechnological and biomedical applications. However, the yield productivity of BGs have encountered limitations, hindering their large-scale preparation multi-faceted applications BGs. Further optimization is needed commercial application BG technology. In this study, we screened out most effective lysis protein ID52-E-W4A among 13 mutants based on phage ID52 E optimized liquid culture medium preparing Escherichia coli Nissle 1917 (EcN). The results revealed a significantly higher rate compared to that ID52-E in 2xYT medium. Furthermore, EcN were cultivated fermenter, achieving an initial OD600 high 6.0 after optimization, indicating enhanced production. Moreover, ID52-E-W4A-induced reached 67.0%, contrasting only 3.1% from φX174-E-induced extended applicability was demonstrated through Salmonella pullorum choleraesuis ghosts. Knocking molecular chaperone gene slyD dnaJ ID52-mediated could still undergo lysis. Conversely, overexpression integral membrane enzyme mraY resulted loss activity ID52-E, suggesting may no longer rely SlyD or DnaJ function, MraY potentially being target ID52-E. This study introduces novel approach utilizing recombinant expression, accelerating formation thereby enhancing productivity.

Language: Английский

The synergy of recombinant NSP4 and VP4 from porcine rotavirus elicited a strong mucosal response DOI
Sufen Li,

Xuechao Tang,

Jinzhu Zhou

et al.

Virology, Journal Year: 2024, Volume and Issue: 597, P. 110130 - 110130

Published: June 5, 2024

Language: Английский

Citations

4
Genetically Engineered Bacterial Ghosts as Vaccine Candidates Against Klebsiella pneumoniae Infection DOI Creative Commons
S. V. Dentovskaya, A. S. Vagaiskaya, A. S. Trunyakova

et al.

Vaccines, Journal Year: 2025, Volume and Issue: 13(1), P. 59 - 59

Published: Jan. 10, 2025

Background/Objectives Bacterial ghosts (BGs), non-living empty envelopes of bacteria, are produced either through genetic engineering or chemical treatment retaining the shape their parent cells. BGs considered vaccine candidates, promising delivery systems, and adjuvants. The practical use in development for humans is limited because concerns about preservation viable bacteria BGs. Methods: To increase efficiency Klebsiella pneumoniae BG formation and, accordingly, to ensure maximum killing we exploited previously designed plasmids with lysis gene E from bacteriophage φX174 holin–endolysin systems λ L-413C phages. Previously, this kit made it possible generate bacterial cells Yersinia pestis varying degrees hydrolysis variable protective activity. Results: In current study, showed that co-expression holin endolysin genes phage elicited more rapid efficient K. than mediated by only single low functioning system phage. introduction alternative lysing factors into instead protein leads loss murein skeleton. resulting frameless cell envelops reminiscent sacs skins Although such structures less naive classical ghosts, they provide effective protection against infection a hypervirulent strain can be recommended as candidate vaccines. For our generated using O1:K2 strain, both safety immunogenicity aspects were evaluated. Humoral cellular immune responses significantly increased mice intraperitoneally immunized compared subcutaneously vaccinated animals (p < 0.05). Conclusions: Therefore, study presents novel perspectives future research on ghost

Language: Английский

Citations

0
Immunization with Plant-based Vaccine Expressing Toxoplasma gondii SAG1 Fused to Plant HSP90 Elicits Protective Immune Response in Lambs DOI

Lucía M. Campero,

Ignacio Gual,

Valeria A. Sander

et al.

Acta Tropica, Journal Year: 2025, Volume and Issue: 262, P. 107540 - 107540

Published: Jan. 31, 2025

Language: Английский

Citations

0
Construction and Mechanism Exploration of Highly Efficient System for Bacterial Ghosts Preparation Based on Engineered Phage ID52 Lysis Protein E DOI Creative Commons
Yi Ma, Sijia Wang, Bin Hong

et al.

Vaccines, Journal Year: 2024, Volume and Issue: 12(5), P. 472 - 472

Published: April 28, 2024

Bacterial ghosts (BGs) are hollow bacterial cell envelopes with intact cellular structures, presenting as promising candidates for various biotechnological and biomedical applications. However, the yield productivity of BGs have encountered limitations, hindering their large-scale preparation multi-faceted applications BGs. Further optimization is needed commercial application BG technology. In this study, we screened out most effective lysis protein ID52-E-W4A among 13 mutants based on phage ID52 E optimized liquid culture medium preparing Escherichia coli Nissle 1917 (EcN). The results revealed a significantly higher rate compared to that ID52-E in 2xYT medium. Furthermore, EcN were cultivated fermenter, achieving an initial OD600 high 6.0 after optimization, indicating enhanced production. Moreover, ID52-E-W4A-induced reached 67.0%, contrasting only 3.1% from φX174-E-induced extended applicability was demonstrated through Salmonella pullorum choleraesuis ghosts. Knocking molecular chaperone gene slyD dnaJ ID52-mediated could still undergo lysis. Conversely, overexpression integral membrane enzyme mraY resulted loss activity ID52-E, suggesting may no longer rely SlyD or DnaJ function, MraY potentially being target ID52-E. This study introduces novel approach utilizing recombinant expression, accelerating formation thereby enhancing productivity.

Language: Английский

Citations

1