Biomolecules,
Journal Year:
2023,
Volume and Issue:
14(1), P. 46 - 46
Published: Dec. 29, 2023
Plasmodium
is
an
obligate
intracellular
parasite
that
has
numerous
interactions
with
different
hosts
during
its
elaborate
life
cycle.
This
also
the
case
for
other
parasites
belonging
to
same
phylum
Apicomplexa.
In
this
study,
we
bioinformatically
identified
components
of
multi-synthetase
complexes
(MSCs)
several
Apicomplexa
and
modelled
their
assembly
using
AlphaFold2.
It
appears
none
these
MSCs
resemble
two
have
characterized
in
Plasmodium.
Indeed,
tRip,
central
protein
involved
association
from
homologues,
suggesting
tRip-dependent
import
exogenous
tRNAs
not
conserved
apicomplexan
parasites.
Based
on
observation,
searched
obvious
differences
could
explain
singularity
synthesis
by
comparing
tRNA
genes
amino
acid
usage
genomes.
We
noted
a
contradiction
between
large
number
asparagine
residues
used
proteomes
single
gene
encoding
inserts
them
into
proteins.
observation
remains
true
all
Plasmodia
strains
studied,
even
those
do
contain
long
homorepeats.
Research Square (Research Square),
Journal Year:
2023,
Volume and Issue:
unknown
Published: Dec. 18, 2023
Abstract
Broadly
cross-reactive
anti-malarial
vaccines
and
therapeutic
interventions
are
needed
to
achieve
better
outcomes
in
controlling
and,
eventually,
eradicating
malaria.
Apical
membrane
antigen-1
(AMA1)
is
a
structurally
functionally
conserved
malarial
vaccine
candidate
involved
the
tight
junction
formation
with
rhoptry
neck
protein
(RON)
complex
at
host
cell-parasite
interface.
This
interaction
crucial
for
all
Plasmodium
parasites
invade
human
erythrocytes,
hepatocytes
mosquito
salivary
glands
effectively.
However,
extensive
surface
polymorphisms
have
induced
P.
falciparum
strain-specific
protection
which
has
so
far
hindered
progression
of
AMA1-based
beyond
first
clinical
trial.
Here,
we
identified
humanised
single-domain
(i-body)
that
recognises
pan-species
conformational
epitope
AMA1
low
nanomolar
affinity
inhibits
binding
RON2
ligand
AMA1.
Structural
characterisation
indicated
WD34
i-body
engages
centre
hydrophobic
cleft
AMA1,
where
interacting
residues
highly
among
species
other
apicomplexans.
Further,
showed
merozoite
invasion
into
RBCs
by
multiple
Plasmodium
species
hepatocyte
sporozoites.
Our
work
describes
identification
biologic
high
multi-lifecycle
stage
inhibitory
activity.
identifies
new
tools
species-independent
immunoprophylaxis
possible
target
structure-based
development
against
bioRxiv (Cold Spring Harbor Laboratory),
Journal Year:
2023,
Volume and Issue:
unknown
Published: Aug. 10, 2023
ABSTRACT
Plasmodium
is
an
obligate
intracellular
parasite
that
makes
numerous
interactions
with
different
hosts
during
its
elaborate
life
cycle.
This
also
the
case
for
other
parasites
belong
to
same
phylum
Apicomplexa
.
In
this
study,
we
identified
bioinformatically
components
of
multi-synthetase
complexes
(MSC)
several
parasites.
By
using
AlphaFold2
modeling
compare
their
assembly,
it
appears
none
these
MSCs
resemble
those
in
particular,
discrepancies
between
core
complexes,
tRip
and
homologs
indicate
tRip-dependent
exogenous
tRNA
import
not
conserved
Based
on
observation,
looked
obvious
differences
could
explain
singularity
The
content
genes
amino
acid
usage
genomes
highlighted
originality
Plasmodia
translation.
evident
respect
asparagine
acid,
which
highly
used
proteomes,
scarcity
Asn
required
protein
synthesis,
regardless
long
homorepeats
or
AT
genomes.
3 Biotech,
Journal Year:
2023,
Volume and Issue:
13(10)
Published: Sept. 12, 2023
Abstract
Utilizing
transcriptomics,
promising
methods
for
identifying
unique
genes
associated
with
Plasmodium
gametocyte
development
offer
a
potential
avenue
novel
candidate
targets
in
transmission
blocking
vaccine
development.
In
this
review,
we
identified
40
publicly
available
transcriptomic
datasets
related
to
parasite
factors
linked
sexual
stage
transmission,
from
which
analyzed
two
RNA-Seq
identify
crucial
the
of
P.
falciparum
humans
mosquito
vectors.
Differential
expression
analysis
revealed
3500
(2489
upregulated
and
1011
downregulated)
common
differentially
expressed
throughout
occurring
both
(gametocyte
II,
V)
mosquitoes
(ookinete).
Among
1283
(914
369
826
(719
107
were
specific
female
male
gametocytes,
respectively.
Also,
830
transition
that
may
be
involved
adaptation
survival
between
human
stages.
Additionally,
reviewed
functional
aspects
important
highly
pathway
evaluated
their
suitability
as
candidates.
The
review
provides
researchers
insight
into
importance
critical
markers
aid
rational
strategies.
Biomolecules,
Journal Year:
2023,
Volume and Issue:
14(1), P. 46 - 46
Published: Dec. 29, 2023
Plasmodium
is
an
obligate
intracellular
parasite
that
has
numerous
interactions
with
different
hosts
during
its
elaborate
life
cycle.
This
also
the
case
for
other
parasites
belonging
to
same
phylum
Apicomplexa.
In
this
study,
we
bioinformatically
identified
components
of
multi-synthetase
complexes
(MSCs)
several
Apicomplexa
and
modelled
their
assembly
using
AlphaFold2.
It
appears
none
these
MSCs
resemble
two
have
characterized
in
Plasmodium.
Indeed,
tRip,
central
protein
involved
association
from
homologues,
suggesting
tRip-dependent
import
exogenous
tRNAs
not
conserved
apicomplexan
parasites.
Based
on
observation,
searched
obvious
differences
could
explain
singularity
synthesis
by
comparing
tRNA
genes
amino
acid
usage
genomes.
We
noted
a
contradiction
between
large
number
asparagine
residues
used
proteomes
single
gene
encoding
inserts
them
into
proteins.
observation
remains
true
all
Plasmodia
strains
studied,
even
those
do
contain
long
homorepeats.
