Applications of advances in mRNA-based platforms as therapeutics and diagnostics in reproductive technologies

Frontiers in Cell and Developmental Biology, Journal Year: 2023, Volume and Issue: 11

Published: May 26, 2023

The recent COVID-19 pandemic led to many drastic changes in not only society, law, economics, but also science and medicine, marking for the first time when drug regulatory authorities cleared use mRNA-based vaccines fight against this outbreak. However, while indeed representing a novel application of such technology context vaccination introducing RNA into cells produce resultant molecules (proteins, antibodies, etc.) is principle. It has been common practice introduce/inject mRNA oocytes embryos inhibit, induce, identify several factors research context, aspects have proposed as potential therapeutic diagnostic applications combat infertility humans. Herein, we describe key areas where platforms thus far represented clinical applications, describing advantages limitations applications. Finally, discuss how advances platforms, driven by pandemic, may stand benefit treatment We present brief future directions could utilise current advancements enhance therapeutics within reproductive biology, specifically with relation oocyte embryo delivery.

Language: Английский

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Nanoscale Technologies in the Fight against COVID-19: From Innovative Nanomaterials to Computer-Aided Discovery of Potential Antiviral Plant-Derived Drugs

Biomolecules, Journal Year: 2022, Volume and Issue: 12(8), P. 1060 - 1060

Published: July 30, 2022

The last few years have increasingly emphasized the need to develop new active antiviral products obtained from artificial synthesis processes using nanomaterials, but also derived natural matrices. At same time, advanced computational approaches found themselves fundamental in repurposing of therapeutics or for reducing very long developing phases drugs discovery, which represents a real limitation, especially case pandemics. first part review is focused on most innovative nanomaterials promising both field therapeutic agents, as well measures control virus spread (i.e., textiles). second aims show how computer-aided technologies can allow us identify, rapid and therefore constantly updated way, plant-derived molecules those included terpenoids) potentially able efficiently interact with SARS-CoV-2 cell penetration pathways.

Language: Английский

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Acute Kidney Injury and Blood Purification Techniques in Severe COVID-19 Patients

Journal of Clinical Medicine, Journal Year: 2022, Volume and Issue: 11(21), P. 6286 - 6286

Published: Oct. 25, 2022

Although most patients with severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) experience manifestations, multi-organ dysfunction is frequent. Almost 20% of hospitalized SARS-CoV-2 infection develop kidney injury (AKI). The pathophysiology AKI a result both the direct and indirect effects infection, including systemic inflammatory responses, activation renin-angiotensin-aldosterone system (RAAS), endothelial coagulative dysfunction. Underlying infection-associated AKI, an immunological hyper-response unbalanced innate adaptative response defined as "cytokine storm" has emerged. Numerous agents have been tested in effort to mitigate cytokine storm, range extracorporeal removal techniques proposed potential therapeutic options. In present review, we summarize main pathogenetic mechanisms underlying COVID-19-related order provide appropriate individual strategy improve clinical outcomes limit progression early disease.

Language: Английский

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Hypochloremia: A Potential Indicator of Poor Outcomes in COVID-19

Medicina, Journal Year: 2024, Volume and Issue: 60(9), P. 1414 - 1414

Published: Aug. 29, 2024

Background: Coronavirus Disease-2019 (COVID-19) has posed formidable challenges to healthcare systems. Exploring novel biomarkers that can provide valuable prognostic insights, particularly in critically ill patients, a significant importance. Against this backdrop, our study aims elucidate the associations between serum chloride levels and clinical outcomes. Methods: A total of 499 patients were enrolled into study. The upon hospital admission recorded then categorized three groups (hypochloremia, normochloremia, hyperchloremia) for evaluation Additionally, C-reactive protein, procalcitonin, D-dimer measurements further evaluation. Results: 390 (78.1%) tested positive COVID-19 via polymerase chain reaction testing. Non-contrast thorax computed tomography scans indicative compatibility all patients. 210 (42%) female 289 (58%) male. 214 (42.8%) necessitated tocilizumab intervention; 250 (50.1%) at an intensive care unit (ICU), with 166 (66.4%) them receiving tocilizumab. 65 (13%) died, 40 (61.5%) whom received tocilizumab; 41 (63%) ICU. Serum markedly lower elevated apparent users, requiring ICU care, who died. Conclusions: findings robust evidence supporting value as biomarker

Language: Английский

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COVID-19 Lung Injury: Unique and Familiar Aspects of Pathophysiology

Applied Sciences, Journal Year: 2024, Volume and Issue: 14(23), P. 11048 - 11048

Published: Nov. 27, 2024

Acute lung injury (ALI), diagnosed clinically as acute respiratory distress syndrome (ARDS), refers to a spectrum of inflammatory processes culminating in increased permeability the pulmonary alveolar–capillary barrier and impaired gas exchange. The pandemic caused by novel coronavirus, SARS-CoV-2, has raised questions similarities differences between COVID-19 ALI other etiologies. This review summarizes current knowledge regarding pathophysiology draws comparisons latter infectious etiologies ALI. Indeed, severe is characterized unique array disease mechanisms including suppression interferon responses, widespread inflammasome activation, altered leukocyte phenotypes, hyperactive thrombotic activity. Moreover, these manifest clinical progression, which further differentiates from viral pathogens such SARS, MERS, influenza. These features bear important implications for future therapeutic strategies.

Language: Английский

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