D-DI/PLT can be a prognostic indicator for sepsis

PeerJ, Journal Year: 2023, Volume and Issue: 11, P. e15910 - e15910

Published: Sept. 5, 2023

Aims To investigate the indicators affecting early outcome of patients with sepsis and to explore its prognostic efficacy for sepsis. Methods We collected clinical data from 201 admitted emergency department Xijing Hospital between June 2019 2022. The were categorized into groups (survival or fatality) based on their 28-day prognosis. characteristics, biochemical indexes, organ function-related indicators, disease scores analyzed both groups. Risk factor analysis was conducted significant differences. Results Among differences deceased survival groups, D-dimer (D-DI), Sequential Organ Failure Assessment (SOFA) score, platelet (PLT), international normalized ratio (INR), D-DI/PLT identified as independent risk factors prognosis patients. Receiver operating characteristic (ROC) curves showed that (area under curve (AUC) = 93.9), D-DI (AUC 89.6), PLT 81.3), SOFA 78.4) had good judgment efficacy. Further, Kaplan Meier (K-M) indicated rates significantly decreased when they high levels D-DI/PLT, D-DI, well low PLTs. hazard (HR) two largest (HR 16.19). Conclusions may be an poor in a predictor patient

Language: Английский

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Complementary Sets of Autoantibodies Induced by SARS-CoV-2, Adenovirus and Bacterial Antigens Cross-React with Human Blood Protein Antigens in COVID-19 Coagulopathies

International Journal of Molecular Sciences, Journal Year: 2022, Volume and Issue: 23(19), P. 11500 - 11500

Published: Sept. 29, 2022

COVID-19 patients often develop coagulopathies including microclotting, thrombotic strokes or thrombocytopenia. Autoantibodies are present against blood-related proteins cardiolipin (CL), serum albumin (SA), platelet factor 4 (PF4), beta 2 glycoprotein 1 (β2GPI), phosphodiesterases (PDE), and coagulation factors such as Factor II, IX, X von Willebrand (vWF). Different combinations of autoantibodies associate with different coagulopathies. Previous research revealed similarities between blood clotting functions SARS-CoV-2 proteins, adenovirus, bacterial associated moderate-to-severe infections. This study investigated whether polyclonal antibodies (mainly goat rabbit) these viruses bacteria recognize human proteins. Antibodies adenovirus recognized vWF, PDE PF4 also additional antigens. Most tested (group A streptococci [GAS], staphylococci, Escherichia coli [E. coli], Klebsiella pneumoniae, Clostridia, Mycobacterium tuberculosis) cross-reacted CL PF4. while GAS bound to F2, VIII, E. PDE. All cross-reactive interactions involved antibody-antigen binding constants smaller than 100 nM. Since most coagulopathy display along CL, viral infections appear be necessary initiate their autoimmune

Language: Английский

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Characterization of extracellular vesicles in COVID-19 infection during pregnancy

Frontiers in Cell and Developmental Biology, Journal Year: 2023, Volume and Issue: 11

Published: July 25, 2023

Introduction: SARS-CoV-2 infection may cause a severe inflammatory response, inflicting morbidity and mortality. This risk is modestly increased in pregnant patients. Despite the hypercoagulability immunosuppression associated with pregnancy, most women experience mild COVID-19 infection. Maternal extracellular vesicles (EVs) interact endothelial immune components to facilitate favorable disease course. pilot study aimed explore characteristics of EVs released during occurring third trimester pregnancy. Methods: In this prospective study, blood samples were obtained from 16 healthy non-pregnant (NP), 18 healthy-pregnant (HP), 22 positive subjects (CoV-P). Disease course pregnancy outcomes assessed characterized. Of note, limited volumes sample acquired made it necessary use smaller different subsets for each analysis. Results: The majority (91%) COVID-19-pregnant (18 2 moderate disease) experienced good pregnancy-related outcomes. EV concentrations higher compared (p = 0.0041) lower 0.0150). CD63 exosome marker expression was 0.0149, p 0.0028, respectively). Similar levels entry proteins (ACE-2 TMPRSS2) found all three groups. Cytokine content subject-EVs EVs, while IL-2 IL-6 decreased 0.043, 0.0390, CD8+, cytotoxic T-cell marker, 0.0108, < 0.0001, COVID-19- demonstrated platelet activation (CD62P) than 0.0327) 0.0365). Endothelial EV-CD144+ versus 0.0093), but similar subjects. Other EVs' coagulation markers/activity, D-Dimer fibrinogen Conclusion: subjects' an attenuated no additional system.

Language: Английский

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Hematological Questions in Personalized Management of COVID-19 Vaccination

Journal of Personalized Medicine, Journal Year: 2023, Volume and Issue: 13(2), P. 259 - 259

Published: Jan. 30, 2023

Coronavirus disease 2019 (COVID-19), caused by severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), has been causing a worldwide pandemic since 2019. Many vaccines have manufactured and shown promising results in reducing morbidity mortality. However, variety of vaccine-related adverse effects, including hematological events, reported, such as thromboembolic thrombocytopenia, bleeding. Moreover, new syndrome, vaccine-induced immune thrombotic following vaccination against COVID-19 recognized. These hematologic side effects also raised concerns about SARS-CoV-2 patients with preexisting conditions. Patients tumors are at higher risk infection, the efficacy safety this group remain uncertain attention. In review, we discuss events disorders.

Language: Английский

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Obesity fosters severe disease outcomes in a mouse model of coronavirus infection associated with transcriptomic abnormalities

Authorea (Authorea), Journal Year: 2024, Volume and Issue: unknown

Published: March 13, 2024

Obesity has been identified as an independent risk factor for severe outcomes in humans with coronavirus disease 2019 (COVID-19) and other infectious diseases. Here, we established a mouse model of COVID-19 using the murine betacoronavirus, hepatitis virus 1 (MHV-1). C57BL/6 C3H/HeJ mice exposed to MHV-1 developed mild disease, respectively. Obese clinical manifestations similar those lean controls. In contrast, all obese succumbed by 8 days post-infection, compared 50% mortality rate Notably, both lung lesions consistent human COVID-19, marked evidence diffuse alveolar damage (DAD). To identify early predictive biomarkers worsened mice, sequenced RNA from whole blood 2 post-infection assessed changes gene pathway expression. Many pathways uniquely altered aligned found COVID-19. Furthermore, observed expression related unfolded protein response lipid metabolism infected their counterparts, suggesting role severity outcomes. This study presents novel studying elucidating mechanisms underlying hosts.

Language: Английский

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The role of lipid metabolism and immune response in the pathogenesis of Ankylosing spondylitis and Primary glaucoma: A Bidirectional Mendelian Randomization Study

Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown

Published: March 18, 2024

Abstract Background Ankylosing Spondylitis (AS) is known to be associated with secondary glaucoma, largely due complications of acute anterior uveitis and corticosteroid use. However, the relationship between AS primary glaucoma more complex has yet fully elucidated. To investigate this further, we used Bidirectional Mendelian randomization (MR) analysis evaluate potential causal further elucidate possible pathogenic mechanisms. Methods We estimated effect ankylosing spondylitis on using a bidirectional approach. In addition assessing direct link, also explored mediation effects lipid metabolism immune response. ensure robustness our findings, supplemented series sensitivity analyses reverse MR. Results suggest that related an increased risk (odds ratio: 1.20, 95% confidence interval:1.03–1.40; P = 0.018). Lipid including very low-density lipoprotein (VLDL) (P < 0.01, β 0), response platelet count 1.0E-06, > IgD unswitched memory B cell CD8 CD39 + T 0) appear mediate glaucoma. Conclusion Our reveals significantly in individuals AS. This heightened suggests interaction potentially mediated by factors These findings provide new insights into mechanisms linking these two conditions.

Language: Английский

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A Review of the Scientific Literature on Experimental Toxicity Studies of COVID-19 Vaccines, with Special Attention to Publications in Specific Toxicology Journals

Published: June 25, 2024

Since the reports of first cases COVID-19, in less than 5 years a huge number documents regarding that disease and coronavirus (SARS-CoV-2), responsible for infection, have been published. The tremendous scientific covers many topics on different issues directly related to COVID-19/SARS-CoV-2. It includes studies about efficacy vaccines prevent disease. However, only comparatively low nonclinical -conducted experimental animals- focused potential adverse/toxic effects COVID-19 vaccines. present review was aimed at revising literature laboratory animals toxic/adverse In addition, investigations reported those specific toxicology journals with highest impact factors examined one by one. results indicate most nonclinical/experimental and/or candidates showed -in general terms- good safety profile. Only some animal were certain adverse found. rather surprising result has limited available (in databases PubMed Scopus) performed companies largest manufacturers mRNA world [https://www.google.com/search?sca_esv=ba085665fbb45bd4&sca_upv=1&q=Who+is+the+largest+manufacturer+of+vaccines+in+the+world%3F&sa=X&sqi=2&ved=2ahUKEwj6nMbAi-qGAxVLaqQEHTPwDcIQzmd6BAgZEAY]. Why these -assuming, course, they conducted- not published journals, allowing judgment international community, including toxicologists?

Language: Английский

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Obesity fosters severe disease outcomes in a mouse model of coronavirus infection associated with transcriptomic abnormalities

Journal of Medical Virology, Journal Year: 2024, Volume and Issue: 96(4)

Published: April 1, 2024

Abstract Obesity has been identified as an independent risk factor for severe outcomes in humans with coronavirus disease 2019 (COVID‐19) and other infectious diseases. Here, we established a mouse model of COVID‐19 using the murine betacoronavirus, hepatitis virus 1 (MHV‐1). C57BL/6 C3H/HeJ mice exposed to MHV‐1 developed mild disease, respectively. Obese clinical manifestations similar those lean controls. In contrast, all obese succumbed by 8 days postinfection, compared 50% mortality rate Notably, both lung lesions consistent human COVID‐19, marked evidence diffuse alveolar damage (DAD). To identify early predictive biomarkers worsened mice, sequenced RNA from whole blood 2 postinfection assessed changes gene pathway expression. Many pathways uniquely altered aligned found COVID‐19. Furthermore, observed expression related unfolded protein response lipid metabolism infected their counterparts, suggesting role severity outcomes. This study presents novel studying elucidating mechanisms underlying hosts.

Language: Английский

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Association of systemic immune-inflammation index with all-cause, cardiovascular and cancer mortality in chronic kidney disease: evidence from NHANES

Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown

Published: April 25, 2024

Objectives We aimed to assess the association between SII levels and risk of all-cause cause-specific mortality in CKD population. Methods The dataset was collected from National Health Nutritional Examination Surveys (NHANES 1999–2014) followed for vital status through December 31, 2015. Cox proportional hazard models were performed evaluate correlation various mortalities [all-cause (ACM)/cardiovascular-related (CVM)/cancer-related (CCM)] levels. Besides, we also further restricted cubic splines (RCS), two-piecewise linear regression, subgroup analysis. Results A total 7035 eligible subjects included. In this cohort, 3279 death participants observed, which 1022 cardiovascular causes, 562 cancer 1659 other causes during long-term follow-up period. After adjusting possible confounders, risks ACM (Q2, Q3, Q4: HR [95 CI%] = 1.05[0.95–1.17], 1.11[1.00-1.23], 1.48[1.34–1.63], P trend < 0.001) CVM 0.96[0.8–1.16], 1.19[0.99,1.42], 1.27[1.06–1.5], an uptrend, along with increasing SII. addition, associated CCM (HR 1.13[1.07–1.19]). RCS analyses demonstrated a J-shaped log (SII) adults CKD, threshold values 2.2, 2.66, 2.6 respectively. Finally, showed that more pronounced females, age ≥ 60 years, body mass index(BMI) > 29.9. Conclusion Increased independent factor ACM, CVM, Furthermore, found curvilinear relationship (ACM/CVM/CCM), 2.6,

Language: Английский

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Reducing Delayed Diagnosis and Overtreatment in Heparin-Induced Thrombocytopenia by Using a Machine-Learning Model: A Prospective, Multicenter, Observational Study

SSRN Electronic Journal, Journal Year: 2022, Volume and Issue: unknown

Published: Jan. 1, 2022

Background: Diagnosing the life-threatening disease heparin-induced thrombocytopenia (HIT) at bedside remains challenging, exposing a significant number of patients risk delayed diagnosis or dangerous overtreatment. We hypothesized that machine-learning algorithms could be utilized to develop more accurate and user-friendly diagnostic tool integrates diverse clinical laboratory information accounts for complex interactions. Methods: conducted prospective cohort study including 1’393 with suspected HIT from 10 centers. Detailed data were collected, various immunoassays conducted. The washed platelet activation assay (HIPA) served as reference standard. Findings: HIPA diagnosed in 119 (prevalence 8.5%). feature selection process training dataset (75% patients) yielded following predictor variables: (1) immunoassay test result, (2) nadir, (3) unfractionated heparin use, (4) CRP, (5) timing thrombocytopenia, (6) other causes thrombocytopenia. best performing models support vector machine case chemiluminescent (CLIA) ELISA, well gradient boosting particle-gel (PaGIA). In validation (25% patients), AUROC all was 0.99 (95% CI: 0.97, 1.00). Compared currently recommended algorithm (4Ts score, immunoassay), numbers false-negative reduced 12 6 (-50.0%; ELISA), 9 3 (-66.7%, PaGIA) 14 5 (-64.3%; CLIA). false-positive individuals 87 61 (-29.8%; 200 63 (-68.5%; increased 50 (+ 29.0 %) CLIA. Interpretation: Our substantially than (https://toradi-hit.org). It has potential reduce overtreatment practice. Future studies shall validate this model wider settings.Funding: Swiss National Science Foundation (SNSF), International Society on Thrombosis Haemostasis (ISTH).Declaration Interest: AC consultant Synergy; received authorship royalties UpToDate; his institution research behalf Alexion, Bayer, Novartis, Novo Nordisk, Pfizer, Sanofi, Spark, Takeda. MN grants Bayer Healthcare, Roche diagnostics, Siemens healthineers, Pentapharm, Bühlmann laboratories, outside current work. All authors declare no conflict interest exists.Ethical Approval: protocol approved by ethical committees (Kantonale Ethikkommission Bern, #2017-01073).

Language: Английский

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