Frontiers in Oncology,
Journal Year:
2023,
Volume and Issue:
13
Published: Aug. 28, 2023
Lung
cancer
is
one
of
the
most
devastating
diseases
worldwide
with
high
incidence
and
mortality,
continues
to
rise.
Metastasis
leading
cause
death
in
lung
patients,
yet
molecular
effectors
underlying
tumor
dissemination
remain
poorly
defined.
Research
findings
recent
years
confirmed
primed
microenvironment
future
metastatic
sites,
called
pre‐metastatic
niche,
a
prerequisite
for
overt
metastasis.
Exosomes
have
recently
emerged
as
important
players
niche
formation.
Natural
medicines
traditionally
been
rich
sources
drug
discovery.
Some
them
exhibit
favorable
anti-lung
activity.
The
review
focused
on
latest
advances
regulation
formation
by
contents
exosomes
representative
natural
medicines.
Additionally,
mechanism
was
summarized
detail,
which
would
provide
new
insights
anti-cancer
development.
Frontiers in Cell and Developmental Biology,
Journal Year:
2025,
Volume and Issue:
12
Published: Jan. 30, 2025
WWP1,
a
member
of
the
C2-WW-HECT
E3
ligase
family,
is
an
ubiquitin-protein
containing
WW
domains.
This
enzyme
plays
critical
role
in
regulating
diverse
cellular
processes.
Its
expression
modulated
by
various
factors
and
non-coding
RNAs,
resulting
ubiquitination
that
affects
substrate
protein
degradation.
WWP1
demonstrates
dual
function,
acting
predominantly
as
oncogene
tumors
but
occasionally
tumor
suppressor.
review
summarizes
WWP1’s
biological
roles,
therapeutic
potential
oncology,
upstream
regulatory
factors,
downstream
substrates.
It
aims
to
promote
research
on
antitumor
effects,
improve
understanding
its
tumorigenesis,
support
development
targeted
therapies.
Frontiers in Immunology,
Journal Year:
2023,
Volume and Issue:
14
Published: May 10, 2023
Colorectal
cancer
(CRC)
is
a
common
malignant
tumor
of
the
digestive
system,
and
its
morbidity
rates
are
increasing
worldwide.
Cancer-associated
fibroblasts
(CAFs),
as
part
microenvironment
(TME),
not
only
closely
linked
to
normal
fibroblasts,
but
also
can
secrete
variety
substances
(including
exosomes)
participate
in
regulation
TME.
Exosomes
play
key
role
intercellular
communication
by
delivering
intracellular
signaling
(e.g.,
proteins,
nucleic
acids,
non-coding
RNAs),
an
number
studies
have
shown
that
RNAs
exosomal
origin
from
CAFs
associated
with
formation
CRC
microenvironment,
increase
ability
grow
metastasis,
mediate
immunosuppression,
involved
mechanism
drug
resistance
patients
receiving.
It
after
radiotherapy
patients.
In
this
paper,
we
review
current
status
progress
research
on
CAFs-derived
CRC.
Journal of Extracellular Vesicles,
Journal Year:
2023,
Volume and Issue:
12(12)
Published: Dec. 1, 2023
Abstract
Natural
killer
cell‐derived
extracellular
vesicles
(NK‐EVs)
have
shown
promising
potential
as
biotherapeutics
for
cancer
due
to
their
unique
attributes
cytotoxic
nanovesicles
against
cells
and
immune‐modulatory
activity
towards
immune
cells.
However,
a
biomanufacturing
workflow
is
needed
produce
clinical‐grade
NK‐EVs
pre‐clinical
clinical
applications.
This
study
established
novel
using
closed‐loop
hollow‐fibre
bioreactor
continuously
from
the
clinically
relevant
NK92‐MI
cell
line
under
serum‐free,
Xeno‐free
feeder‐free
conditions
following
GMP‐compliant
conditions.
The
NK92
grown
in
three
continuous
production
lots
resulted
large
quantities
of
both
NK
NK‐EV
at
end
each
lot
(over
10
9
viable
13
EVs),
while
retaining
payload
(granzyme
B
perforin),
pro‐inflammatory
cytokine
(interferon‐gamma)
content
cytotoxicity
human
leukemic
K562
with
limited
off‐target
toxicity
healthy
fibroblast
scalable
has
facilitate
translation
adoptive
cell‐based
NK‐EV‐based
immunotherapies
GMP
considerations.
Analytical Methods,
Journal Year:
2024,
Volume and Issue:
16(39), P. 6634 - 6653
Published: Jan. 1, 2024
Along
with
discovering
cancer
biomarkers,
non-invasive
detection
methods
have
played
a
critical
role
in
early
diagnosis
and
prognostic
improvement.
Some
traditional
been
used
for
detecting
but
they
are
time-consuming
involve
materials
human
costs.
With
great
flexibility,
sensitivity
specificity,
the
clustered
regularly
interspaced
short
palindromic
repeats
(CRISPR)-associated
system
provides
wide
range
of
application
prospects
this
field.
Herein,
we
introduce
background
CRISPR-Cas
(CRISPR-associated)
comprehensively
summarize
strategies
mediated
by
system,
including
four
kinds
biochemical-based
markers:
nucleic
acid,
enzyme,
tumor-specific
protein
exosome.
Furthermore,
discuss
challenges
implementing
clinical
applications.
The FASEB Journal,
Journal Year:
2024,
Volume and Issue:
38(13)
Published: June 26, 2024
Abstract
Exosomes
play
significant
roles
in
the
communications
between
tumor
cells
and
microenvironment.
However,
specific
mechanisms
by
which
exosomes
modulate
development
under
hypoxia
pancreatic
neuroendocrine
tumors
(pNETs)
are
not
well
understood.
This
study
aims
to
investigate
these
made
several
important
discoveries.
We
found
that
hypoxic
derived
from
pNETs
can
activate
tumor‐associated
macrophages
(TAM)
M2
phenotype,
turn,
M2‐polarized
TAM,
facilitate
migration
invasion
of
cells.
Further
investigation
revealed
CEACAM5,
a
protein
highly
expressed
cells,
is
enriched
cell‐derived
exosomes.
Hypoxic
exosomal
CEACAM5
was
observed
induce
polarization
TAM
through
activation
MAPK
signaling
pathway.
Coculturing
with
or
treated
enhanced
metastatic
capacity
In
conclusion,
findings
suggest
generate
CEACAM5‐rich
microenvironment,
turn
polarize
promote
malignant
Targeting
could
potentially
serve
as
diagnostic
therapeutic
strategy
for
pNETs.
BIO-PROTOCOL,
Journal Year:
2023,
Volume and Issue:
13(11)
Published: Jan. 1, 2023
Exosomes
are
lipid
bilayer-enclosed
vesicles,
actively
secreted
by
cells,
containing
proteins,
lipids,
nucleic
acids,
and
other
substances
with
multiple
biological
functions
after
entering
target
cells.
derived
from
NK
cells
have
been
shown
to
certain
anti-tumor
effects
potential
applications
as
chemotherapy
drug
carriers.
These
developments
resulted
in
high
demand
for
exosomes.
Although
there
has
large-scale
industrial
preparation
of
exosomes,
they
only
generally
engineered
such
HEK
293T.
The
specific
cellular
exosomes
is
still
a
major
problem
laboratory
studies.
Therefore,
this
study,
we
used
tangential
flow
filtration
(TFF)
concentrate
the
culture
supernatants
isolated
cell-derived
(NK-Exo)
ultracentrifugation.
Through
series
characterization
functional
verification
NK-Exo,
characterization,
phenotype,
activity
NK-Exo
were
verified.
Our
study
provides
considerably
time-
labor-saving
protocol
isolation
NK-Exo.
Smart Materials in Medicine,
Journal Year:
2023,
Volume and Issue:
4, P. 639 - 647
Published: Jan. 1, 2023
Sepsis
and
sepsis-related
organ
dysfunction
have
been
identified
as
significant
global
life-threatening
health
threats,
with
a
high
mortality
rate
despite
ongoing
research
in
the
area.
Timely
diagnosis
is
essential
such
that
treatment
could
be
initiated
early
possible
to
ensure
best
outcome,
since
delayed
intervention
associated
higher
mortality.
Patient
stratification
disease
monitoring,
present
challenges
sepsis
management
strategies,
largely
due
heterogenicity
of
signs
symptoms.
Hence
focus
on
potential
biomarkers
overcome
these
needed.
Recently,
extracellular
vesicles
(EVs),
mainly
exosome
subtype,
investigated
regarding
their
role
diagnostics,
therapeutics
drug
delivery
vehicles.
Herein,
we
an
up-to-date
review
covering
circulating
exosomes
monitoring
progression
for
sepsis.
To
provide
context,
pathophysiology
highlighted.
Future
prospects,
current
recommendations
are
also
identified.
Oncology Reports,
Journal Year:
2024,
Volume and Issue:
53(1)
Published: Nov. 20, 2024
Pancreatic
ductal
adenocarcinoma
(PDAC)
is
a
malignant
neoplasm
that
typically
manifests
with
subtle
clinical
manifestations
in
its
early
stages
and
frequently
eludes
diagnosis
until
the
advanced
phases
of
disease.
The
limited
therapeutic
options
available
for
PDAC
significantly
contribute
to
high
mortality
rate,
highlighting
urgent
need
novel
biomarkers
capable
effectively
identifying
facilitating
precise
diagnosis.
pivotal
role
cellular
exosomes
both
pathogenesis
interventions
has
been
underscored.
Furthermore,
researchers
have
acknowledged
potential
as
targeted
drug
carriers
against
regulatory
cells
treating
PDAC.
present
article
aims
provide
comprehensive
review
encompassing
recent
advancements
utilizing
elucidating
mechanisms
underlying
disease
development,
patterns
metastasis,
diagnostic
techniques
treatment
strategies
associated
