Proteomics analysis of lung tissue reveals protein makers for the lung injury of adjuvant arthritis rats DOI Creative Commons

Pingheng Zhang,

Danbin Wu,

Jian Liu

et al.

Molecular Medicine Reports, Journal Year: 2023, Volume and Issue: 28(3)

Published: July 11, 2023

Lung injury is one of the common extra‑articular lesions in rheumatoid arthritis (RA). Due to its insidious onset and no obvious clinical symptoms, it can be easily dismissed early stage diagnosis, which reasons that leads a decline quality life subsequent death patients with RA. However, pathogenesis still unclear there lack effective therapeutic targets. In present study, tandem mass tag‑labeled proteomics was used research lung tissue proteins RA model (adjuvant arthritis, AA) rats had secondary injury. The aim study identify differentially expressed related RA‑lung injury, determine their potential role provide targets for treatment. samples were collected from AA‑lung normal rats. (DEPs) identified by spectrometry. Bioinformatic analysis assess biological processes signaling pathways associated these DEPs. A total 310 DEPs found, 244 upregulated 66 downregulated. KEGG anlysis showed 'fatty acid degradation', metabolism', elongation', 'complement coagulation cascades', 'peroxisome proliferator‑activated receptor pathway' 'hypoxia‑inducible factor significantly tissues Immunofluorescence staining confirmed increased expression clusterin, serine protease inhibitors complement 1qc AA results revealed significance certain (for example, C9, C1qc Clu) occurrence development provided support through experiments biomarkers diagnosis prevention

Language: Английский

Organ crosstalk and dysfunction in sepsis DOI Creative Commons
André Borges, Luís Bento

Annals of Intensive Care, Journal Year: 2024, Volume and Issue: 14(1)

Published: Sept. 19, 2024

Language: Английский

Citations

8
Baicalin relieves complement alternative pathway activation-induced lung inflammation through inhibition of NF-κB pathway DOI Creative Commons
Jiao Li,

Qi-Yun Zhang,

Qing‐Yu Lu

et al.

BMC Complementary Medicine and Therapies, Journal Year: 2024, Volume and Issue: 24(1)

Published: Sept. 13, 2024

Language: Английский

Citations

4
Overview on the Link Between the Complement System and Auto-Immune Articular and Pulmonary Disease DOI Creative Commons
Paola Triggianese, Paola Conigliaro, Erica De Martino

et al.

Open Access Rheumatology Research and Reviews, Journal Year: 2023, Volume and Issue: Volume 15, P. 65 - 79

Published: May 1, 2023

Abstract: Complement system (CS) dysregulation is a key factor in the pathogenesis of different autoimmune diseases playing central role many immune innate and adaptive processes. Rheumatoid arthritis (RA) chronic inflammatory disease characterized by ta breach self-tolerance leading to synovitis extra-articular manifestations. The CS activated RA seems not only mediate direct tissue damage but also play initiation pathogenetic mechanisms through interactions with citrullinated proteins. Interstitial lung (ILD) represents most common manifestation that can lead progressive fibrosis. In this review, we focused on evidence ILD, highlighted both responses development diseases, using idiopathic pulmonary fibrosis as model disease. As proof concept, dissected several treatments used treat ILD such glucocorticoids, pirfenidone, modifying antirheumatic drugs, targeted biologics tumor necrosis (TNF)-inhibitors, rituximab, tocilizumab, nintedanib may act indirectly CS, suggesting might represent potential therapeutic target these complex diseases. Keywords: complement system, rheumatoid arthritis, interstitial disease, therapies

Language: Английский

Citations

10
Autophagy-related biomarkers identified in sepsis-induced ARDS through bioinformatics analysis DOI Creative Commons
Wei Wang, Jianfeng Zhao, Hui Li

et al.

Scientific Reports, Journal Year: 2025, Volume and Issue: 15(1)

Published: March 6, 2025

While dysregulated autophagy has been linked to acute respiratory distress syndrome (ARDS) development in sepsis, the exact regulatory mechanisms driving this process remain unclear. This study systematically investigated autophagy-related genes sepsis-induced ARDS using integrative bioinformatics, including weighted gene coexpression network analysis (WGCNA), differential expression (DEGs), receiver operating characteristic (ROC) curve analysis, Gene Ontology (GO) and Kyoto Encyclopedia of Genes Genomes (KEGG) enrichment protein‒protein interaction (PPI) immune infiltration analysis. Hub were further validated by qPCR Beas-2B cells receiving lipopolysaccharide (LPS) stimulation. We identified 18 DEGs with diagnostic potential for ARDS. These endocytosis, protein kinase inhibition, enigmatic Ficolin-1-rich granules. The downregulated hallmark signaling pathways involved apoptosis, complement, IL-2/STAT5, KRAS signaling. Immune revealed alterations 7 cell subsets, CD8 + T-cell exhaustion, natural killer reduction, type 1 helper response. When treated LPS, we discovered that 6 out hub significantly downregulated. Our findings provide novel insights into autophagy-mediated pathogenesis sepsis. represent promising candidates clinical biomarker therapeutic targeting, which necessitates validation.

Language: Английский

Citations

0
Targeting neutrophil dysfunction in acute lung injury: insights from active components of Chinese medicine DOI

Saiya Ye,

Lin Ma, Yayun Chi

et al.

Phytomedicine, Journal Year: 2025, Volume and Issue: unknown, P. 156664 - 156664

Published: March 1, 2025

Language: Английский

Citations

0
Circulatory HMGB1 is an early predictive and prognostic biomarker of ARDS and mortality in a swine model of polytrauma DOI Creative Commons
Matthew D. Young,

Tomas S. Cancio,

Catherine R. Thorpe

et al.

Frontiers in Immunology, Journal Year: 2023, Volume and Issue: 14

Published: July 14, 2023

Acute respiratory distress syndrome (ARDS) is a leading cause of morbidity and mortality in polytrauma patients. Pharmacological treatments ARDS are lacking, patients rely on supportive care. Accurate diagnosis vital for early intervention improved outcomes but presently delayed up to days. The use biomarkers identification development potential solution. Inflammatory mediators high-mobility group box 1 (HMGB1), syndecan-1 (SDC-1), C3a have been previously proposed as biomarkers. For this study, we analyzed these animals undergoing smoke inhalation 40% total body surface area burns, followed by intensive care 72 h post-injury (PI) determine their association with mortality. We found that the levels inflammatory serum were affected, well degree HMGB1 Toll-like receptor 4 (TLR4) signal activation lung. results showed significantly increased expression developed compared those did not. Receiver operating characteristic (ROC) analysis at 6 PI associated (AUROC=0.77) (AUROC=0.82). Logistic regression revealed ≥24.10 ng/ml 13-fold higher incidence [OR:13.57 (2.76–104.3)], whereas ≥31.39 12-fold increase [OR: 12.00 (2.36–93.47)]. In addition, mesenchymal stem cell (MSC) therapeutic treatment led significant decrease systemic elevation failed block SDC-1 increases. Immunohistochemistry analyses burn injury induced TLR4 stimulated co-localization Interestingly, MSC reduced presence HMGB1, TLR4, HMGB1-TLR4 co-localization. These show prognostic biomarker predicting swine injury. Therapeutically blocking might be an effective approach attenuating combat casualties or civilian

Language: Английский

Citations

4
Research hotspots and evolving trends of barrier dysfunction in acute lung injury and acute respiratory distress syndrome DOI Creative Commons

Zixin Luo,

Xinyue Song, Duoqin Huang

et al.

Heliyon, Journal Year: 2024, Volume and Issue: 10(9), P. e30579 - e30579

Published: May 1, 2024

Endothelial and epithelial barrier dysfunction due to increased permeability heightened inflammatory reactions influences the emergence of acute lung injury (ALI) respiratory distress syndrome (ARDS). Nevertheless, bibliometric research comparing endothelial barriers is limited. Therefore, this study analyzed Web Science Core Collection (WoSCC) Citation Index Expanded literature explore present priorities development tendencies within field. We conducted a comprehensive search (October 18, 2023) on WoSCC from January 1, 2010, October 2023, focusing articles related in ALI ARDS. Retrieved data were visualized using R-bibliometrix, VOS viewer 1.6.19, CiteSpace 6.2. R4. Functional enrichment analysis gene targets identified keyword list Kyoto Encyclopedia Genes Genomes (KEGG) Gene ontology databases, based STRING database construct PPI network predict core genes. A total 941 original reviews identified. The United States had highest number publications citations H-index G-index. According Collaboration Network Analysis graph, China strongest collaboration. Birukova AA most among all authors, while eight top ten institutions with mediator centrality located States.

Language: Английский

Citations

1
DMPP attenuates lipopolysaccharide-induced lung injury by inhibiting glycocalyx degradation through activation of the cholinergic anti-inflammatory pathway DOI
Qi Feng, Chengwei Duan, Tianpeng Chen

et al.

Journal of Bioenergetics and Biomembranes, Journal Year: 2023, Volume and Issue: 55(6), P. 447 - 456

Published: Oct. 18, 2023

Language: Английский

Citations

1
Impact of immunopathy and coagulopathy on multi-organ failure and mortality in a lethal porcine model of uncontrolled hemorrhagic shock DOI Open Access

Milomir O. Simovic,

James A. Bynum, Bin Liu

et al.

Published: Dec. 18, 2023

Background: Uncontrolled hemorrhage is a major preventable cause of death in patients with trauma. However, the majority large animal models have utilized controlled rather than uncontrolled to investigate impact immunopathy and coagulopathy on multi-organ failure (MOF) mortality. This study evaluates these alterations severe porcine-controlled hemorrhagic shock (HS) model. Methods: Anesthetized female swine underwent (22 ml/kg) by partial splenic resection followed or without lactated Ringer solution (LR) Voluven® resuscitation. Blood chemistry, physiologic variables, systemic tissue levels complement proteins cytokines, coagulation parameters, organ function, damage were recorded assessed. Results: HS resulted local activation, cytokine release, metabolic acidosis, hyperkalemia, MOF, no survival. Resuscitation LR after improved hemodynamic parameters (MAP SI), survival but increased activation worse coagulopathy. Compared group, treated had dilutional anemia, coagulopathy, renal hepatic dysfunction, myocardial damage, decreased rate. Conclusions: Hemorrhagic triggers early that appear associated MOF death. indicates are therapeutic targets may be addressed high-impact adjunctive treatment conventional

Language: Английский

Citations

1
Impact of Immunopathy and Coagulopathy on Multi-Organ Failure and Mortality in a Lethal Porcine Model of Controlled and Uncontrolled Hemorrhage DOI Open Access

Milomir O. Simovic,

James A. Bynum, Bin Liu

et al.

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(5), P. 2500 - 2500

Published: Feb. 21, 2024

Uncontrolled hemorrhage is a major preventable cause of death in patients with trauma. However, the majority large animal models have utilized controlled rather than uncontrolled to investigate impact immunopathy and coagulopathy on multi-organ failure (MOF) mortality. This study evaluates these alterations severe porcine hemorrhagic shock (HS) model. Anesthetized female swine underwent by partial splenic resection followed or without lactated Ringer solution (LR) Voluven

Language: Английский

Citations

0