Dr Jekyll and Mr Hyde: From Two Branches of Immune Response to Three Types of Interferon Response DOI Open Access
Brent Brown

Published: Aug. 9, 2023

Interferons were the original prototype cytokine system discovered during research of 20th century. As name suggests these originally considered to be synthesised and secreted between cells. However, technological advancements since dictate processes involved in secreting proteins can extensively explained through both genetic biochemical pathways comparatively clearer. Interferon (IFN) discovery occurred when was its infancy. Simultaneous by Franklin Wilkins deoxyribonucleic acid (DNA) structure function with Crick Watson concurrently; however, two scientists Isaac Lindemann described first IFN 1957. Technological advancement allows comparison, many pathogens mutations factors regulation. Cancer cell regulation has long been central host synthesis and/or affected differential protein subunits defined further acting 6 domains. Type II remains immune as it is released a myriad cells, mainly Natural Killer T cells commonly used quantitative measurement adaptive cellular immunity. Single–stranded double–stranded RNA DNA viruses well bacterial infections (e.g., Escherichia) fungal Mycobacteria) affect systems. These utilise intra/extracellular like Toll–like Receptors (TLRs) within overall transduction pathways. Questions remain over immunological mechanisms contributing innate type III 2003 phenotypes characterised further. Changes I/II/III IFNs may alter homeostatic differentially beneficially pathological disorders. We therefore aim present rationale this regulatory mechanism action context developments recently functional effects

Language: Английский

Physiological functions of RIG-I-like receptors DOI Creative Commons
Mitsutoshi Yoneyama, Hiroki Kato, Takashi Fujita

et al.

Immunity, Journal Year: 2024, Volume and Issue: 57(4), P. 731 - 751

Published: April 1, 2024

Language: Английский

Citations

19
The IFN-stimulated gene IFI27 counteracts innate immune responses after viral infections by interfering with RIG-I signaling DOI Creative Commons
Laura Villamayor, Darío López-García,

Vanessa Rivero

et al.

Frontiers in Microbiology, Journal Year: 2023, Volume and Issue: 14

Published: April 28, 2023

The recognition of viral nucleic acids by host pattern receptors (PRRs) is critical for initiating innate immune responses against infections. These are mediated the induction interferons (IFNs), IFN-stimulated genes (ISGs) and pro-inflammatory cytokines. However, regulatory mechanisms to avoid excessive or long-lasting that may cause detrimental hyperinflammation. Here, we identified a novel function ISG, IFN alpha inducible protein 27 (IFI27) in counteracting triggered cytoplasmic RNA binding. Our model systems included three unrelated infections caused Influenza A virus (IAV), Severe Acute Respiratory Syndrome coronavirus 2 (SARS-CoV-2), Sendai (SeV), transfection with an analog double-stranded (ds) RNA. Furthermore, found IFI27 has positive effect on IAV SARS-CoV-2 replication, most likely due its ability counteract host-induced antiviral responses, including vivo . We also show interacts PRR retinoic acid-inducible gene I (RIG-I), being interaction RIG-I through Interestingly, our results indicate impairs activation, providing molecular mechanism modulating responses. study identifies explain counterbalancing preventing Therefore, this will have important implications drug design control viral-induced pathology.

Language: Английский

Citations

29
The crucial regulatory role of type I interferon in inflammatory diseases DOI Creative Commons
Ling Ji, Tianle Li,

Huimin Chen

et al.

Cell & Bioscience, Journal Year: 2023, Volume and Issue: 13(1)

Published: Dec. 20, 2023

Type I interferon (IFN-I) plays crucial roles in the regulation of inflammation and it is associated with various inflammatory diseases including systemic lupus erythematosus (SLE), rheumatoid arthritis (RA), periodontitis, impacting people's health quality life. It well-established that IFN-Is affect immune responses factors by regulating some signaling. However, currently, there no comprehensive overview regulatory role IFN-I distinctive pathways as well diseases. This review aims to provide a narrative involvement different signaling pathways, mainly mediating related key specific targets cascades influence progression As such, we suggested induce through stimulation certain which displays possible efficient treatment methods provides reference for precise control

Language: Английский

Citations

29
The antiviral state of the cell: lessons from SARS-CoV-2 DOI
Jérémie Le Pen, Charles M. Rice

Current Opinion in Immunology, Journal Year: 2024, Volume and Issue: 87, P. 102426 - 102426

Published: April 1, 2024

Language: Английский

Citations

7
Toxicity and absorption of polystyrene micro-nanoplastics in healthy and Crohn’s disease human duodenum-chip models DOI Creative Commons
Davood Kharaghani, Glen M. DeLoid, Ping He

et al.

Journal of Hazardous Materials, Journal Year: 2025, Volume and Issue: 490, P. 137714 - 137714

Published: Feb. 24, 2025

Language: Английский

Citations

0
Single cell transcriptome sequencing of stimulated and frozen human peripheral blood mononuclear cells DOI Creative Commons

Céline Derbois,

Marie-Ange Palomares,

Jean‐François Deleuze

et al.

Scientific Data, Journal Year: 2023, Volume and Issue: 10(1)

Published: July 6, 2023

Abstract Peripheral blood mononuclear cells (PBMCs) are that a critical part of the immune system used to fight off infection, defending our bodies from harmful pathogens. In biomedical research, PBMCs commonly study global response disease outbreak and progression, pathogen infections, for vaccine development multitude other clinical applications. Over past few years, revolution in single-cell RNA sequencing (scRNA-seq) has enabled an unbiased quantification gene expression thousands individual cells, which provides more efficient tool decipher human diseases. this work, we generate scRNA-seq data at high depth (>100,000 reads/cell) than 30,000 resting, stimulated, fresh frozen conditions. The generated can be benchmarking batch correction integration methods, effect freezing-thawing cycles on quality cell populations their transcriptomic profiles.

Language: Английский

Citations

11
Multi-Omics Analysis Reveals the IFI6 Gene as a Prognostic Indicator and Therapeutic Target in Esophageal Cancer DOI Open Access
Nguyen-Kieu Viet-Nhi, Tran Minh Quan, Vu Cong Truc

et al.

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(5), P. 2691 - 2691

Published: Feb. 26, 2024

The role of the IFI6 gene has been described in several cancers, but its involvement esophageal cancer (ESCA) remains unclear. This study aimed to identify novel prognostic indicators for ESCA-targeted therapy by investigating IFI6's expression, epigenetic mechanisms, and signaling activities. We utilized public data from Gene Expression Omnibus (GEO) Cancer Genome Atlas (TCGA) analyze clinical characteristics, function, pathways, correlation with different immune cells ESCA. TIMER2.0 database was employed assess pan-cancer expression IFI6, while UALCAN used examine across tumor stages histology subtypes. Additionally, KEGG helped related pathways. Our findings revealed 95 genes positively correlated 15 negatively over-expressed ESCA other impacting patient survival showing higher tissues than normal tissues. also CD4+ T B cell receptors (BCRs), both essential response. GO Biological Process (GO BP) enrichment analysis indicated that primarily associated Type I interferon pathway defense response viruses. Intriguingly, demonstrated were mostly linked Cytosolic DNA-sensing pathway, which plays a crucial innate immunity viral defense, RIG-I-like receptor (RLR) detects infections activates responses. Pathways various identified. It is important note our relied on online databases. Given consists two distinct subgroups (ESCC EAC), most databases combine them into single category. Future research should focus evaluating impact each subgroup gain more specific insights. In conclusion, inhibiting using targeted could be an effective strategy treating considering potential as biomarker factors.

Language: Английский

Citations

4
Harnessing Molecular Insights for Translational Impact: Highlights from the Special Issue Titled “New Insights in Translational Bioinformatics” DOI Open Access
Camelia Quek

International Journal of Molecular Sciences, Journal Year: 2025, Volume and Issue: 26(2), P. 579 - 579

Published: Jan. 11, 2025

The field of translational bioinformatics is rapidly evolving, driving the convergence molecular sciences and computational methods with their applications in industrial clinical practice [...]

Language: Английский

Citations

0
RNA sequencing reveals key factors modulating TNFa-stimulated odontoblast-like differentiation of dental pulp stem cells DOI Creative Commons
Muhammad Irfan, Jihyun Kim,

Sreelekshmi Sreekumar

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 14, 2025

Abstract Inflammation is a complex host response to harmful infections or injuries, playing both beneficial and detrimental roles in tissue regeneration. Notably, clinical dentinogenesis associated with caries development occurs within an inflammatory environment. Reparative closely linked intense inflammation, which triggers the recruitment differentiation of dental pulp stem cells (DPSCs) into dentin lineage. Understanding how responses influence DPSCs essential for elucidating mechanisms underlying Given limited data on this process, broad approach employed here gain deeper understanding involved identify downstream signaling targets. This study aims investigate role inflammation complement receptor C5L2 odontoblastic transcriptomic changes using poly-A RNA sequencing (RNA-seq). RNA-seq techniques provide insight transcriptome cell, offering higher coverage greater resolution its dynamic nature. Following stimulation, exhibit significantly altered gene profiles, including marked upregulation key odontogenic genes, highlighting critical dentinogenesis. We demonstrate that TNFα-treated odontoblast-like differentiating DPSCs, under modulation, significant differential expression changes. The presented may new avenues experimental approaches uncover pathways by identifying specific transcription factors profiles.

Language: Английский

Citations

0
Human long noncoding RNA VILMIR is induced by major respiratory viral infections and modulates the host interferon response DOI Creative Commons
K John,

Ian Huntress,

Ethan D. Smith

et al.

Journal of Virology, Journal Year: 2025, Volume and Issue: unknown

Published: March 25, 2025

ABSTRACT Long noncoding RNAs (lncRNAs) are a newer class of transcripts identified as key regulators biological processes. Here, we aimed to identify novel lncRNA targets that play critical roles in major human respiratory viral infections by systematically mining large-scale transcriptomic data sets. Using bulk RNA-sequencing (RNA-seq) analysis, previously uncharacterized lncRNA, named virus-inducible modulator interferon response ( VILMIR ), was consistently upregulated after vitro influenza infection across multiple epithelial cell lines and A virus subtypes. also severe acute syndrome coronavirus 2 (SARS-CoV-2) syncytial (RSV) . We experimentally confirmed the interferon-beta (IFN-β) treatment A549 line found expression robustly induced IFN-β dose- time-specific manner. Single-cell RNA-seq analysis bronchoalveolar lavage fluid samples from disease 2019 (COVID-19) patients uncovered various types, including at least five immune cells. The upregulation cells further T monocyte lines, SUP-T1 THP-1, treatment. Finally, knockdown reduced magnitude host transcriptional responses both Together, our results show is interferon-stimulated gene (ISG) regulates may be potential therapeutic target for upon mechanistic investigation. IMPORTANCE Identifying factors regulate developing new therapeutics. Human long have been regulatory during processes; however, majority functions within antiviral remain unknown. In this study, influenza, 2, virus. demonstrated an several types. Our reveal present infections.

Language: Английский

Citations

0