Gut-derived immune cells and the gut-lung axis in ARDS

Critical Care, Journal Year: 2024, Volume and Issue: 28(1)

Published: July 4, 2024

Abstract The gut serves as a vital immunological organ orchestrating immune responses and influencing distant mucosal sites, notably the respiratory mucosa. It is increasingly recognized central driver of critical illnesses, with intestinal hyperpermeability facilitating bacterial translocation, systemic inflammation, damage. “gut-lung” axis emerges pivotal pathway, where gut-derived injurious factors trigger acute lung injury (ALI) through circulation. Direct indirect effects microbiota significantly impact responses. Dysbiosis, particularly dysbiosis, termed an imbalance microbial species reduction in diversity within certain bodily microbiomes, influences adaptive responses, including differentiating T regulatory cells (Tregs) helper 17 (Th17) cells, which are various inflammatory conditions. Additionally, bone marrow pulmonary activity, underscoring complex gut-lung interplay. Moreover, alterations implicated diverse pathologies, affecting local landscapes. Notably, dysbiosis can reciprocally influence composition, indicating bidirectional communication. In this review, we investigate pathophysiology ALI/acute distress syndrome (ARDS), elucidating role based on recent experimental clinical research. This exploration aims to enhance understanding ALI/ARDS pathogenesis underscore significance interactions diseases.

Language: Английский

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Stroke and myocardial infarction induce neutrophil extracellular trap release disrupting lymphoid organ structure and immunoglobulin secretion

Nature Cardiovascular Research, Journal Year: 2024, Volume and Issue: 3(5), P. 525 - 540

Published: April 23, 2024

Post-injury dysfunction of humoral immunity accounts for infections and poor outcomes in cardiovascular diseases. Among immunoglobulins (Ig), IgA, the most abundant mucosal antibody, is produced by plasma B cells intestinal Peyer's patches (PP) lamina propria. Here we show that patients with stroke myocardial ischemia (MI) had strongly reduced IgA blood levels. This was phenocopied experimental mouse models where decreased fecal were accompanied rapid loss IgA-producing PP Reduced IgG detectable mice 3-10 d after injury. Stroke/MI triggered release neutrophil extracellular traps (NETs). Depletion neutrophils, NET degradation or blockade inhibited

Language: Английский

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Physiological and immunological barriers in the lung

Seminars in Immunopathology, Journal Year: 2024, Volume and Issue: 45(4-6), P. 533 - 547

Published: Jan. 1, 2024

Abstract The lungs serve as the primary organ for respiration, facilitating vital exchange of gases with bloodstream. Given their perpetual exposure to external particulates and pathogens, they possess intricate protective barriers. Cellular adhesion in is robustly maintained through tight junctions, adherens desmosomes. Furthermore, pulmonary system features a mucociliary clearance mechanism that synthesizes mucus transports it outside. This enriched chemical barriers like antimicrobial proteins immunoglobulin A (IgA). Additionally, complex immunological network comprising epithelial cells, neural immune cells plays pivotal role defense. comprehensive understanding these systems offers valuable insights into potential pathologies therapeutic interventions.

Language: Английский

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Unmasking the potential of secretory IgA and its pivotal role in protection from respiratory viruses

Antiviral Research, Journal Year: 2024, Volume and Issue: 223, P. 105823 - 105823

Published: Feb. 6, 2024

Language: Английский

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SIgA in various pulmonary diseases

European journal of medical research, Journal Year: 2023, Volume and Issue: 28(1)

Published: Aug. 27, 2023

Abstract Secretory immunoglobulin A (SIgA) is one of the most abundant subtypes among mucosa, which plays an indispensable role in first-line protection against invading pathogens and antigens. Therefore, respiratory SIgA mucosal immune diseases has attracted more attention. Although intestinal immunity been widely studied, cell types responsible for interactions between cells are still unclear. Here, we conducted a wide search relevant studies sorted out relationship some pulmonary (COPD, asthma, tuberculosis, idiopathic fibrosis, COVID-19, lung cancer), found involved pathogenesis progression various diseases, intending to provide new ideas prevention, diagnosis, treatment related diseases.

Language: Английский

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Enhancing quality and yield of recombinant secretory IgA antibodies in Nicotiana benthamiana by endoplasmic reticulum engineering

Plant Biotechnology Journal, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 16, 2025

Summary The production of complex multimeric secretory immunoglobulins (SIgA) in Nicotiana benthamiana leaves is challenging, with significant reductions complete protein assembly and consequently yield, being the most important difficulties. Expanding physical dimensions ER to mimic professional antibody‐secreting cells can help increase yields promote folding assembly. Here, we expanded N. by targeting enzyme CTP:phosphocholine cytidylyltransferase (CCT), which catalyses rate‐limiting step synthesis key membrane component phosphatidylcholine (PC). We used CRISPR/Cas perform site‐directed mutagenesis each three endogenous CCT genes introducing frame‐shifting indels remove auto‐inhibitory C‐terminal domains. generated stable homozygous lines containing different combinations edited genes, including plants where all isofunctional homologues were modified. Changes morphology mutant confirmed vivo confocal imaging substantially increased two fully assembled SIgAs prolonging residence time boosting chaperone accumulation. Through a combination engineering overexpression, SIgA an order magnitude, reaching almost 1 g/kg fresh leaf weight. This strategy removes major roadblock producing will likely facilitate other biopharmaceutical proteins plants.

Language: Английский

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Exploring the Mucosal Immune Response in Axial Spondyloarthritis Through Immunoglobulin A-Coated Microbiota

Rheumatic Disease Clinics of North America, Journal Year: 2025, Volume and Issue: 51(2), P. 283 - 293

Published: March 3, 2025

Language: Английский

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Intravenous immunoglobulin in kidney transplantation: Mechanisms of action, clinical applications, adverse effects, and hyperimmune globulin

Clinical Immunology, Journal Year: 2023, Volume and Issue: 256, P. 109782 - 109782

Published: Sept. 22, 2023

Language: Английский

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Clinical efficacy of IgM-enriched immunoglobulin as adjunctive therapy in neonatal and pediatric sepsis: a systematic review and meta-analysis

Frontiers in Pediatrics, Journal Year: 2023, Volume and Issue: 11

Published: Aug. 11, 2023

Background Sepsis is a major cause of mortality and morbidity globally, with around one-quarter all sepsis-related deaths occurring in children under the age 5. We conducted meta-analysis systematic review literature to evaluate clinical effectiveness an IgM-enriched immunoglobulin preparation pediatrics patients neonates sepsis. Methods Systematic searches PubMed, Cochrane Library Embase databases were performed November 2022, no date limitations, identify studies which was used as adjunctive therapy neonatal pediatric Results In total, 15 fulfilled eligibility criteria, 13 2 studies. Pooled estimates from indicated that rates significantly lower who received treatment compared controls (OR 0.41; 95% CI 0.32–0.55). Further analyses studies, alone, showed significant benefit longer durations (>3 days) vs. recommended duration (3 0.32; 0.22–0.47) 0.61; 0.41–0.92). Treatment associated risk prospective retrospective 0.37; 0.27–0.51) 0.73; 0.41–1.30). Conclusions This suggests may reduce populations. However, large randomized controlled trials are required further substantiate these findings.

Language: Английский

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Asymmetric N-Glycosylation in the Tailpiece of Recombinant IgA1

Journal of the American Chemical Society, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 6, 2024

Here, we employed a variety of mass spectrometry (MS)-based approaches, both (glyco)peptide-centric and protein-centric, to resolve the complex glycoproteoform landscape recombinant IgA1 produced in HEK293 cells. These key immunoglobulins harbor several N- O-glycosylation sites, making them considerably more heterogeneous than their IgG counterparts. We provide quantitative data on occupancy glycan composition for each glycosylation site. Combining all data, revealed that molecules consist at least three distinct populations with varying N-glycosylation site occupancies C-terminal tailpiece, namely, one sites occupied, another unoccupied, third asymmetric population occupied other challenging prevailing acceptance is symmetrical. This finding significant, given tailpiece involved interactions J-chain Polymeric Immunoglobulin Receptor, general as antibody quality attribute needs be carefully monitored, presence nature these modifications can affect antibody's efficacy, lifetime, stability, binding and/or neutralizing capacities. Optimizing strategies produce requires efficient specific control analytical strategies, presented here, which essential therapeutic IgA1-based development. expect integrated MS-based strategy here may beneficial comprehensively characterize profiles therapeutics, thereby improving production optimization processes facilitating pathway bring therapeutics into clinical applications.

Language: Английский

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