Changes in intestinal flora of mice induced by rEg.P29 epitope peptide vaccines

Immunity Inflammation and Disease, Journal Year: 2023, Volume and Issue: 11(11)

Published: Nov. 1, 2023

Cystic echinococcosis (CE), a zoonotic parasitic disease caused by Echinococcus granulosus, remains public health and socioeconomic issue worldwide, making its prevention treatment of vital importance. The aim this study was to investigate changes in the intestinal microbiota mice immunized with three peptide vaccines based on recombinant antigen E. P29 (rEg.P29), hope providing more valuable information for development against CE. Three vaccines, rEg.P29T , rEg.P29B + B were prepared rEg.P29, subcutaneous immunization model established. floras different groups analyzed 16 S rRNA gene sequencing. analysis at both time points revealed that Firmicutes, Bacteroidota, Verrucomicrobiota predominant flora phylum level, while genus Akkermansia, unclassified_Muribaculaceae, Lachnospiraceae_NK4A136_group, uncultured_rumen bacterium dominant genera. Some probiotics intestines significantly increased after such as Lactobacillus_taiwanensis, Lactobacillus_reuteri, Bacteroides_acidifaciens, so forth. Meanwhile, some harmful or conditionally pathogenic bacteria decreased, Turicibacter sanguinis, Desulfovibrio_fairfieldensis, Clostridium_sp, forth, most which are associated inflammatory infectious diseases. Kyoto Encyclopaedia Genes Genomes enrichment differential enriched multiple metabolic pathways, primarily biological systems, human diseases, metabolism, cellular processes, environmental processing. In study, we comprehensively compared well their related theoretical background novel granulosus.

Language: Английский

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Expansion of Proteome-wide Coxiella burnetii Comparative T-cell Epitope Prediction to Include Small Ruminant Hosts

Journal of Cellular Immunology, Journal Year: 2023, Volume and Issue: 5(5), P. 143 - 161

Published: Dec. 22, 2023

Background: Coxiella burnetii is the causative agent of Q fever, a human disease that can be acquired from livestock. Diseases caused by this organism have great losses in livestock and health. No vaccine approved for use United States, formalin-inactivated whole-cell vaccines pose significant manufacturing risk biocontainment. A subunit using recombinant peptides C. would safer less resource-intensive to produce. This study used reverse vaccinology expand our prediction sets T-cell epitopes major histocompatibility complex (MHC) Class I II alleles cattle, sheep, goats. Thereafter, present results were compared with those previous mice humans. Results: Small ruminant breed representation States was ensured querying whole genome sequences on National Center Biotechnology Information database. Consequently, twenty-two sheep MHC I, seventeen goat one added analyses, resulting total fifty-six eighteen II, twenty-seven alleles. Predicted interactions proteome-derived each allele categorized as strong, weak, or non-binding based bioinformatic scores. Interspecies comparisons resulted 256 interest presentation 766 presentation. Of these, 51 predicted bind both classes alleles, which 33 newly identified. Conclusion: The high scoring epitope predictions identified provide grounds prioritizing candidates further develop safe perhaps broadly effective vaccine.

Language: Английский

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Introduction of protein vaccine candidate based on AP65, AP33, and α-actinin proteins against Trichomonas vaginalis parasite: an immunoinformatics design

Parasites & Vectors, Journal Year: 2024, Volume and Issue: 17(1)

Published: March 31, 2024

Abstract Background Trichomonas vaginalis is the most common nonviral sexually transmitted disease (STI) worldwide. Vaccination generally considered to be one of effective methods preventing infectious diseases. Using AP65, AP33 and α-actinin proteins, this research aims develop a protein vaccine against . Methods Based on B-cell T-cell epitope prediction servers, antigenic epitopes were selected, with necessary evaluations, epitope-rich domains three AP33, α-actinin, selected linked. Subsequently, ability interact toll-like receptors 2 4 (TLR2 TLR4) was assessed. The stability interactions also studied by molecular dynamics for duration 100 nanoseconds. Results designed consists 780 amino acids weight 85247.31 daltons. results interaction candidate TLR2 TLR4 immune system showed that there are strong between (-890.7 kcal mol -1 ) (-967.3 ). All parameters evaluate structure protein-TLR2 protein-TLR4 complexes stable alone in complex receptors. Investigation induce an response using C-ImmSim web server capable stimulating B- lymphocytes produce cytokines antibodies Conclusions Overall, our may have potential protection However, experimental vivo vitro studies, it good candidate. Graphical

Language: Английский

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Engineering neoantigens to form immunogenic biopolymer particles targeting metastatic breast cancer

Applied Materials Today, Journal Year: 2024, Volume and Issue: 38, P. 102238 - 102238

Published: May 25, 2024

Cancer vaccines that target tumor-specific neoantigens have shown promising results in recent clinical trials. However, these are still the early stages of development and face significant challenges, including fighting heterogeneous tumors, high costs limited efficacy, restricting their accessibility to cancer patients. To overcome we developed particulate neoantigen formulations by engineering Escherichia coli assemble biopolymer particles (BPs) coated with interest facilitating effective uptake antigen presenting cells induction neoantigen-specific immune responses. We designed, manufactured, tested therapeutic efficacy BPs retroviral glycoprotein 70-derived 4T1 murine metastatic breast model. Neoantigen-BP vaccine were safe effectively reduced tumor progression enhancing infiltration cytotoxic helper T into microenvironment. Tumor- infiltrating displayed levels suppressor marker B lymphocyte attenuator, while expressing higher death ligand FasL. This was aligned neoantigen-BP vaccinated mice developing significantly lower numbers tumors nodules lungs indicating response-mediated suppression metastasis. Histological analysis showed more extensive necrosis tissues groups compared control groups. The addition chekpoint inhibitor, anti-PD1, had synergistic effects on suppression. Overall, findings support feasibility utilizing neoantigen-coated as innovative delivery platform induce anti-tumor responses, mediating progression.

Language: Английский

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A novel oral vaccine delivery system for enhancing stability and immune protection: bacterium-like particle with functional coating

Frontiers in Microbiology, Journal Year: 2024, Volume and Issue: 15

Published: Oct. 30, 2024

Bacterium-like particles (BLPs) have gained significant attention in vaccine development due to their potential as effective immune enhancers and antigen delivery systems. BLPs are generated by boiling lactic acid bacteria an acidic solution devoid of proteins nucleic acids, offering advantages terms ease preparation, high safety, good stability. Furthermore, employing protein anchor (PA), heterogeneous antigens can be efficiently displayed on the surface BLPs, resulting enhanced effectiveness. Despite these benefits, most BLP-based vaccines currently administered via injection or intranasal delivery, with oral remaining limited. This limitation is primarily harsh environment gastrointestinal tract, which degrades particles. To enhance efficacy immunization subunit vaccines, we developed a simple rapid method for self-assembling lipid membrane onto achieving encapsulation efficiency up 99%, combination has biosafety. The novel system not only preserves adjuvant activity but also protects from adverse environments, increasing absorption intestinal Peyer's patches (PPs). Oral was required once, protection after challenge 100%. observed immunity cross-protection. Transcriptome analysis small intestine suggested that enhancement probably exerted promoting transport antigens. Therefore, posit design this new presents approach advancing vaccines.

Language: Английский

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Functionalisation of polyhydroxybutyrate for diagnostic uses

New Biotechnology, Journal Year: 2024, Volume and Issue: 85, P. 9 - 15

Published: Nov. 15, 2024

Polyhydroxybutyrate (PHB) is a biodegradable and biocompatible biopolyester, naturally produced self-assembled as spherical inclusions inside bacteria. These PHB particles contain hydrophobic core covalently coated with synthase (PhaC), which serves an anchoring linker for foreign proteins of interest. Protein engineering PhaC enables the display biologically active protein functions on surface suitable different applications. Many biomolecules, such e.g. antigens, enzymes, fluorescent were immobilized to exhibited superior functionalities when compared their respective soluble counterparts. Recently, have been successfully applied various diagnostics This mini review provides overview unique design space towards development safe cost-effective diagnostic tools, highlights important research progresses manufacturing particles-based diagnostics.

Language: Английский

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