Interaction of the endogenous antibody response with activating FcγRs enhance control of Mayaro virus through monocytes

PLoS Pathogens, Journal Year: 2025, Volume and Issue: 21(2), P. e1012944 - e1012944

Published: Feb. 24, 2025

Mayaro virus (MAYV) is an emerging arbovirus. Previous studies have shown antibody Fc effector functions are critical for optimal monoclonal antibody-mediated protection against alphaviruses; however, the requirement of gamma receptors (FcγRs) during natural infection has not been evaluated. Here, we showed mice lacking activating FcγRs (FcRγ −/− ) developed prolonged clinical disease with increased MAYV in joint-associated tissues. Viral reduction was associated anti-MAYV cell surface binding antibodies rather than neutralizing antibodies. Lack Fc-FcγR engagement number monocytes present tissue through chronic timepoints. Single-cell RNA sequencing elevated levels pro-inflammatory FcRγ and macrophages. Transfer into wild type animals sufficient to increase tissue. Overall, this study suggests that promotes protective responses prevents a pro-viral role monocytes.

Language: Английский

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Antibody‐mediated control mechanisms of viral infections

Immunological Reviews, Journal Year: 2024, Volume and Issue: 328(1), P. 205 - 220

Published: Aug. 20, 2024

Summary Antibodies generated after vaccination or natural pathogen exposure are essential mediators of protection against many infections. Most studies with viruses have focused on antibody neutralization, in which is conferred by the fragment antigen binding region (Fab) through targeting different steps viral lifecycle including attachment, internalization, fusion, and egress. Beyond crystallizable (Fc) antibodies can integrate innate adaptive immune responses engaging complement components distinct Fc gamma receptors (FcγR) host cells. In this review, we discuss recent advances our understanding neutralization effector functions, assays used to measure them. Additionally, describe contexts these mechanisms associated highlight how Fc‐FcγR interactions improve potency antibody‐based therapies.

Language: Английский

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Recent Advances in Arboviral Vaccines: Emerging Platforms and Promising Innovations

Biologics, Journal Year: 2023, Volume and Issue: 4(1), P. 1 - 16

Published: Dec. 22, 2023

Arboviruses are a group of viruses that transmitted by arthropods, such as mosquitoes, and cause significant morbidity mortality worldwide. Currently, there only few options, with restricted use, for effective vaccines against these viruses. However, recent advances in arboviral vaccine development have shown promising innovations potential preclinical clinical studies. Insect-specific been explored novel platform can induce cross-protective immunity related arboviruses. Nanoparticle-based also developed to enhance the immunogenicity stability viral antigens. Additionally, mosquito salivary proteins modulate host immune response interfere transmission being explored. Synonymous recoding, random codon shuffling, deoptimization, codon-pair is investigated strategy attenuate replication arboviruses vertebrate cells, reducing risk reverting wild-type virulence. Finally, mRNA rapidly generate express antigens eliciting robust durable responses. The challenges opportunities outlined, future directions research innovation discussed.

Language: Английский

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Activating FcγRs on monocytes are necessary for optimal Mayaro virus clearance

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: July 24, 2024

Mayaro virus (MAYV) is an emerging arbovirus. Previous studies have shown antibody Fc effector functions are critical for optimal monoclonal antibody-mediated protection against alphaviruses; however, the requirement of gamma receptors (FcγRs) during natural infection has not been evaluated. Here, we showed mice lacking activating FcγRs (FcRγ

Language: Английский

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