Heliyon, Journal Year: 2024, Volume and Issue: 10(14), P. e34530 - e34530
Published: July 1, 2024
Language: Английский
Journal of Nanobiotechnology, Journal Year: 2024, Volume and Issue: 22(1)
Published: Aug. 14, 2024
Abstract Lower back pain (LBP) is a common condition closely associated with intervertebral disc degeneration (IDD), causing significant socioeconomic burden. Inflammatory activation in degenerated discs involves pro-inflammatory cytokines, dysregulated regulatory and increased levels of nerve growth factor (NGF), leading to further destruction sensitization. Macrophage polarization related autophagy. Based on these pathological features, structured biomimetic nanoparticle coated TrkA-overexpressing macrophage membranes (TMNP@SR) rapamycin-loaded mesoporous silica core developed. TMNP@SR acted like sponges adsorbe inflammatory cytokines NGF delivers the autophagy regulator rapamycin (RAPA) into macrophages through homologous targeting effects outer engineered cell membrane. By regulating activation, promoted M1-to-M2 switch avoid continuous inflammation within disc, which prevented apoptosis nucleus pulposus cells. In addition, relieved mechanical thermal hyperalgesia, reduced calcitonin gene-related peptide (CGRP) substance P (SP) expression dorsal root ganglion, downregulated GFAP c-FOS signaling spinal cord rat IDD model. summary, spontaneously inhibits aggravation alleviate reduce ingress sensory nerves, presenting promising treatment strategy for LBP induced by degeneration. Graphical
Language: Английский
Citations
6
Biology, Journal Year: 2023, Volume and Issue: 12(12), P. 1512 - 1512
Published: Dec. 11, 2023
Ozone is an allotrope of oxygen whose use in medicine has rapidly grown recent years. Ozonated blood allows for the ozone a safe modality, as plasma and cells are endowed with antioxidant system able to quench ozone's pro-oxidant property elicit Nrf2/Kwap1/ARE pathway.We present two clinical studies, case-series (six patients) observational study adopting major autohemotherapy topical address infected post-surgical wounds multi-drug resistant bacteria (250 using ozonated treating knee osteoarthritis.Ozonated via reduced extent infections wounds, inflammatory biomarkers by more than 75% improved patients' QoL, whereas minor significantly (p < 0.001) WOMAC Lequesne's parameters osteoarthritis.The models described, i.e., wound antimicrobial treatment osteoarthrosis, following our protocols, share outstanding ability modulate innate immune response bacterial clearance well inflammation pain.
Language: Английский
Citations
11
Frontiers in Medicine, Journal Year: 2024, Volume and Issue: 11
Published: May 22, 2024
Background Knee osteoarthritis (KOA) is a disease that significantly affects the quality of life patients, with complex pathophysiology includes degeneration cartilage and subchondral bone, synovitis, associations mechanical load, inflammation, metabolic factors, hormonal changes, aging. Objective This article aims to comprehensively review biological mechanisms clinical effects general exercise training traditional Chinese exercises (such as Tai Chi Qigong) on treatment KOA, providing references for development prescriptions. Methods A systematic search databases including PubMed, Web Science, Google Scholar, China National Knowledge Infrastructure (CNKI) was conducted, reviewing studies randomized controlled trials (RCTs), observational studies, reviews, meta-analyses. Keywords included “knee osteoarthritis,” “exercise therapy,” “physical activity,” “traditional exercise.” Results conclusion General positively KOA by such promoting blood circulation, improving metabolism inflammatory enhancing expression anti-inflammatory cytokines, reducing cell Traditional exercises, like Qigong, benefit improvement symptoms tissue repair regulating immune function alleviating joint inflammation. Clinical have shown both types can improve physical function, life, pain relief in patients KOA. Both are non-pharmacological options effectively patients’ physiological life. Future research should further explore long-term these interventions develop personalized programs based specific needs patients.
Language: Английский
Citations
4
Neurology International, Journal Year: 2024, Volume and Issue: 16(6), P. 1788 - 1818
Published: Dec. 9, 2024
Animal models are valuable tools for studying the underlying mechanisms of and potential treatments intervertebral disc diseases. In this review, we discuss advantages limitations animal diseases, focusing on lumbar spinal stenosis, herniation, degeneration, as well future research directions. The that they enable controlled experiments, long-term monitoring to study natural history disease, testing treatments. However, also have limitations, including species differences, ethical concerns, a lack standardized protocols, short lifespans. Therefore, ongoing focuses improving model standardization incorporating advanced imaging noninvasive techniques, genetic models, biomechanical analyses overcome these limitations. These directions hold our understanding diseases developing new Overall, although can provide insights into pathophysiology their should be carefully considered when interpreting findings from studies.
Language: Английский
Citations
4
Frontiers in Immunology, Journal Year: 2025, Volume and Issue: 16
Published: April 15, 2025
Aging is characterized by gradual structural and functional changes in the body over time, with intervertebral disc degeneration (IVDD) representing a key manifestation of spinal aging major contributor to low back pain (LBP). This study utilized bioinformatics machine learning approaches identify aging-related biomarkers associated IVDD whole blood samples. By analyzing GEO datasets alongside databases such as GeneCards, HAGR, AgeAnno, we identified 15 differentially expressed genes (AIDEGs). Correlation immune infiltration analyses were conducted on these AIDEGs, diagnostic models developed using WGCNA, logistic regression, random forest, support vector machine, k-nearest neighbors, LASSO regression genes. Among these, FCGR1A, CBS, FASLG emerged significant strong predictive capabilities for IVDD. Further exploration biological pathways involving AIDEGs provided insights into their potential roles pathogenesis. To further validate findings, collected human specimens vitro experiments. ELISA assays confirmed that CBS are crucial IVDD, distinct expression patterns patients moderate versus severe degeneration. These results highlight provide new perspective early intervention treatment strategies
Language: Английский
Citations
0
Frontiers in Endocrinology, Journal Year: 2024, Volume and Issue: 15
Published: March 12, 2024
The intervertebral disc is not isolated from other tissues. Recently, abundant research has linked homeostasis and degeneration to various systemic diseases, including obesity, metabolic syndrome, diabetes. Organokines are a group of diverse factors named for the tissue origin, adipokines, osteokines, myokines, cardiokines, gastrointestinal hormones, hepatokines. Through endocrine, paracrine, autocrine mechanisms, organokines modulate energy homeostasis, oxidative stress, balance in tissues mediate cross-organ communication. These molecules involved regulation cellular behavior, inflammation, matrix metabolism under physiological pathological conditions. In this review, we aimed summarize impact on underlying signaling mechanism. We focused regulatory mechanisms provide basis development early diagnostic therapeutic strategies degeneration.
Language: Английский
Citations
3
Cell Biology and Toxicology, Journal Year: 2025, Volume and Issue: 41(1)
Published: Jan. 18, 2025
This study delved into the molecular mechanisms underlying mechanical stress-induced intervertebral disc degeneration (msi-IDD) through single-cell and high-throughput transcriptome sequencing in mouse models patient samples. Results exhibited an upsurge macrophage presence msi-IDD (IVD) tissues, with secreted phosphoprotein 1 (SPP1) identified as a pivotal driver exacerbating via protein kinase RNA-like endoplasmic reticulum kinase/ activating transcription factor 4/ interleukin-10 (PERK/ATF4/IL-10) signaling axis. Inhibition of SPP1 demonstrated promising outcomes mitigating progression both vitro vivo models. These findings underscore therapeutic promise associated modulation PERK pathway IDD, shedding light on pathogenesis proposing avenue for intervention strategies.
Language: Английский
Citations
0
Cellular Signalling, Journal Year: 2024, Volume and Issue: 121, P. 111277 - 111277
Published: June 28, 2024
Language: Английский
Citations
3
Advanced Healthcare Materials, Journal Year: 2024, Volume and Issue: 13(27)
Published: July 9, 2024
Abstract Pain caused by lumbar disc herniation (LDH) severely compromises patients’ quality of life. The combination steroid and local anesthetics is routinely employed in clinics to alleviate LDH‐induced pain. However, the approach only mediates transient efficacy requires repeated invasive epidural injections. Here a paravertebrally‐injected multifunctional hydrogel that can efficiently co‐load controlled release glucocorticoid betamethasone ropivacaine for sustained anti‐inflammation, reactive oxygen species (ROS)‐removal pain relief LDH presented. Betamethasone conjugated hyaluronic acid (HA) via ROS‐responsive crosslinker form amphiphilic polymer self‐assemble into particles with loaded core. Solution drug‐loaded thermo‐sensitive rapidly forms therapeutic situ upon injection next herniated disc, thus avoiding injection. In rat model LDH, maintains drug concentration 72 times longer than free drugs more effectively inhibits expression pro‐inflammatory cytokines pain‐related molecules including cyclooxygenase‐2 (COX‐2) prostaglandin E2 (PGE 2 ). Therapeutic suppresses rats 12 days while equivalent dose effective 3 days. This platform also applicable ameliorate other spine‐related diseases.
Language: Английский
Citations
3
Advanced Science, Journal Year: 2024, Volume and Issue: 11(44)
Published: Oct. 7, 2024
Abstract In situ disc regeneration is a meticulously orchestrated process, which involves cell recruitment, proliferation and differentiation within local inflammatory niche. Thus far, it remains challenge to establish multi‐staged regulatory framework for coordinating these cellular events, therefore leading unsatisfactory outcome. This study constructs super paramagnetically‐responsive gel, incorporating superparamagnetic iron oxide nanoparticles (SPIONs) aptamer‐modified palladium‐hydrogen nanozymes (PdH‐Apt) into double‐network polyacrylamide/hyaluronic acid (PAAm/HA) hydrogel. The Aptamer DB67 magnetic hydrogel (Mag‐gel) showed high affinity disialoganglioside (GD2), specific membrane ligand of nucleus pulposus stem cells (NPSCs), precisely recruit them the injury site. Mag‐gel exhibits remarkable sensitivity field (MF), exerts tunable micro/nano‐scale forces on recruited NPSCs triggers cytoskeletal remodeling, consequently boosting expansion in early stage. By altering parameters MF, mechanical cues facilitates restore structure later Furthermore, PdH mitigate harsh microenvironment, favoring survival regeneration. presents remote strategy chronologically regulating endogenous fate, supporting without invasive procedures.
Language: Английский
Citations
3