Expert Review of Clinical Immunology, Journal Year: 2024, Volume and Issue: 20(9), P. 1027 - 1039
Published: April 27, 2024
Introduction The COVID-19 pandemic, caused by the SARS-CoV-2 virus, has posed unprecedented global health challenges since its emergence in December 2019. rapid availability of vaccines been estimated to save millions lives, but there is variation how individuals respond vaccines, influencing their effectiveness at an individual, and population level.
Language: Английский
Science Advances, Journal Year: 2025, Volume and Issue: 11(9)
Published: Feb. 26, 2025
Repeated mRNA COVID-19 vaccination increases spike-specific immunoglobulin G4 (IgG4) titers. Here, we characterized the influence of increased IgG4 titers on a range Fc-mediated responses. Elevated reduced binding to FcγRIIIa and decreased antibody-dependent cellular cytotoxicity. However, in individuals with lower total IgG, acted synergy other IgG subclasses improve FcγRI FcγRIIa consequently phagocytosis. Furthermore, this trend was more pronounced recent SARS-CoV-2 variants where induced comparably These observations were further confirmed by silico modeling antibody subclass concentrations FcγR polymorphisms modulated. Collectively, illustrate that impact elevated upon Fc functions is dependent multiple interconnected antigen factors, which should be taken into consideration when dissecting mechanisms driving an effective response following vaccination.
Language: Английский
Citations
0
Clinical & Translational Immunology, Journal Year: 2024, Volume and Issue: 13(3)
Published: Jan. 1, 2024
Abstract Objectives Amino acid variations across more than 30 immunoglobulin (Ig) allotypes may introduce structural changes that influence recognition by anti‐Ig detection reagents, consequently confounding interpretation of antibody responses, particularly in genetically diverse cohorts. Here, we assessed a panel commercial monoclonal anti‐IgG1 clones for capacity to universally recognise two dominant IgG1 haplotypes (G1m‐1,3 and G1m1,17). Methods Four anti‐human were via ELISAs multiplex bead‐based assays their ability bind G1m‐1,3 G1m1,17 variants. Detection antibodies validated against allotype standards tested antigen‐specific plasma from homozygous heterozygous SARS‐CoV‐2 BNT162b2 vaccinated ( n = 28) COVID‐19 convalescent 44) individuals. An Fc‐specific pan ‐IgG corroborated differences between hinge‐ responses. Results Hinge‐specific clone 4E3 preferentially bound compared IgG1. Consequently, Spike‐specific levels detected G1m1,17/G1m1,17 vaccinees appeared 9‐ 17‐fold higher G1m‐1,3/G1m‐1,3 vaccinees. equivalently variants, comparable haplotypes. responses other human coronaviruses influenza similarly poorly subjects. Conclusion Anti‐IgG1 confounds assessment clinical cohorts owing bias towards Validation should include evaluation binding relevant as the role immunogenetics upon humoral immunity is increasingly explored populations.
Language: Английский
Citations
2
Expert Opinion on Biological Therapy, Journal Year: 2023, Volume and Issue: 23(12), P. 1211 - 1217
Published: Dec. 2, 2023
Introduction On 31 July 2023, the United States Department of Health and Human Services announced formation Office Long COVID Research Practice National Institutes (NIH) opened enrollment for therapeutic arm RECOVER initiative, a prospective, randomized study to evaluate new treatment options long coronavirus disease 2019 (long COVID).
Language: Английский
Citations
4
Vaccines, Journal Year: 2024, Volume and Issue: 12(10), P. 1195 - 1195
Published: Oct. 19, 2024
The accumulation of specific IgG antibodies in blood serum is considered a key criterion for the effectiveness vaccination. For several vaccine-preventable infections, quantitative indicators humoral response have been established, which, when reached, provide high probability protection against infection. presence such formal correlate vaccine crucial, example, organizing preventive measures and validating newly developed vaccines. However, can effective infection occur level lower than that provided by parenteral vaccination? Will be sufficient if same antigen administered via mucosal membranes without achieving levels circulating blood?
Language: Английский
Citations
1
Clinical & Translational Immunology, Journal Year: 2023, Volume and Issue: 12(11)
Published: Jan. 1, 2023
Abstract Objectives Tuberculosis (TB) remains a substantial cause of morbidity and mortality among people living with human immunodeficiency virus (HIV) worldwide. However, the immunological mechanisms associated enhanced susceptibility HIV‐positive individuals remain largely unknown. Methods Here, we used simian (SIV)/TB‐coinfection Mauritian cynomolgus macaque (MCM) model to examine humoral responses from plasma SIV‐negative ( n = 8) SIV‐positive 7) MCM 8‐week postinfection Mycobacterium tuberculosis Mtb ). Results Antibody were impaired during SIV coinfection. Elevated inflammatory bulk IgG antibody glycosylation patterns observed in coinfected macaques early at post‐ infection, including increased agalactosylation (G0) reduced di‐galactosylation (G2), which correlated endpoint bacterial burden gross pathology scores, as well time‐to‐necropsy. Conclusion These studies suggest that immunity may contribute control TB disease support growing literature highlights Fc biomarker progression.
Language: Английский
Citations
2
Methods in molecular biology, Journal Year: 2024, Volume and Issue: unknown, P. 201 - 218
Published: Jan. 1, 2024
Language: Английский
Citations
0
Expert Review of Clinical Immunology, Journal Year: 2024, Volume and Issue: 20(9), P. 1027 - 1039
Published: April 27, 2024
Introduction The COVID-19 pandemic, caused by the SARS-CoV-2 virus, has posed unprecedented global health challenges since its emergence in December 2019. rapid availability of vaccines been estimated to save millions lives, but there is variation how individuals respond vaccines, influencing their effectiveness at an individual, and population level.
Language: Английский
Citations
0