A synergistic blend of dietary organic acids, monoglycerides and phytobiotics enhance growth performance, intestinal mucosal height, and anti-viral immune gene expression in juvenile Barramundi (Lates calcarifer)

Aquaculture Reports, Journal Year: 2025, Volume and Issue: 41, P. 102692 - 102692

Published: Feb. 21, 2025

Language: Английский

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Transcriptomic response of lumpfish (Cyclopterus lumpus) head kidney to viral mimic, with a focus on the interferon regulatory factor family

Frontiers in Immunology, Journal Year: 2024, Volume and Issue: 15

Published: Aug. 15, 2024

The economic importance of lumpfish ( Cyclopterus lumpus ) is increasing, but several aspects its immune responses are not well understood. To discover genes and mechanisms involved in the antiviral response, fish were intraperitoneally injected with either viral mimic polyinosinic:polycytidylic acid [poly(I:C)] or phosphate-buffered saline (PBS; vehicle control), head kidneys sampled 24 hours post-injection (hpi) for transcriptomic analyses. RNA sequencing (RNA-Seq) (adjusted p-value <0.05) identified 4,499 upregulated 3,952 downregulated transcripts poly(I:C)-injected compared to PBS-injected fish. Eighteen as differentially expressed by RNA-Seq included a qPCR study that confirmed upregulation encoding proteins response functions (e.g., rsad2 downregulation jarid2b potential cellular process functions. In addition, transcript expression levels 12 members interferon regulatory factor (IRF) family [seven which poly(I:C)-responsive this study] analyzed using qPCR. Levels irf1a , irf1b irf2 irf3 irf4b irf7 irf8 irf9 irf10 significantly higher irf4a irf5 lower This research associated new genomic resources enhance our understanding molecular underlying stimulation help identify possible therapeutic targets biomarkers infections species.

Language: Английский

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Hepatic melanomacrophage centers in the arctic cultured fish Cyclopterus lumpus are not indicative of its health state

Aquaculture, Journal Year: 2023, Volume and Issue: 581, P. 740417 - 740417

Published: Nov. 26, 2023

The lumpfish,

Language: Английский

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Target analyses by transcriptome sequencing in peripheral blood cells of grass carp: Potent immune responses in leukocytes and thrombocytes against bacterial and viral infections

Aquaculture, Journal Year: 2024, Volume and Issue: 592, P. 741211 - 741211

Published: June 12, 2024

Language: Английский

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Analyses of the Mx family members in lumpfish: molecular characterization, phylogeny, and gene expression analyses

Developmental & Comparative Immunology, Journal Year: 2024, Volume and Issue: unknown, P. 105225 - 105225

Published: July 1, 2024

Language: Английский

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Evolutionary, comparative, and functional analyses of STATs and regulation of the JAK-STAT pathway in lumpfish upon bacterial and poly(I:C) exposure

Frontiers in Cellular and Infection Microbiology, Journal Year: 2023, Volume and Issue: 13

Published: Sept. 22, 2023

The Janus kinase/signal transducers and activators of transcription (JAK-STAT) system regulates several biological processes by affecting genes as a response to cytokines growth factors. In the present study, we have characterized STAT in lumpfish (Cyclopterus lumpus L.), belonging order Perciformes, investigated regulation JAK-STAT signaling pathway upon exposure bacteria (Vibrio anguillarum) poly(I:C), latter mimicking antiviral responses.Characterization evolutionary analyses STATs were performed phylogeny, protein domain, homology similarity synteny analyses. Antibacterial responses performing KEGG analysis.We observed that stat1a, 2, 3, 4, 5a, 5b, 6. Transcriptome-wide showed most components lumpfish. il-6, il-10, il-21, iκBα stat3 upregulated 6 hours post (hpe) against while type I interferons (IFNs), irf1, irf3, irf10, stat1 2 24 hpe poly(I:C).Our findings shed light on diversity evolution data show are highly conserved among fish, including transcriptome-wide lay groundwork for future research into functional significance these regulating critical make an important basis development prophylactic measure such vaccination, which is needed since it vulnerable both bacterial viral diseases.

Language: Английский

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Alternative splicing variants of stimulator of interferon genes (STING) from Asian seabass (Lates calcarifer) and their immune response against red spotted grouper nervous necrosis virus (RGNNV)

Developmental & Comparative Immunology, Journal Year: 2023, Volume and Issue: 149, P. 105062 - 105062

Published: Sept. 17, 2023

Language: Английский

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