Targeting the immuno-inflammatory-microbial network: a key strategy for sepsis treatment DOI Creative Commons
Yue Xu, Jiaxin Wang, Rui Yuan

et al.

Frontiers in Immunology, Journal Year: 2025, Volume and Issue: 16

Published: April 14, 2025

Sepsis is a life-threatening condition caused by dysregulated host response to infection, remaining major global health challenge despite clinical advances. Therapeutic challenges arise from antibiotic misuse, incomplete understanding of its complex pathophysiology, and the unresolved interplay immune dysregulation microbiota disruption. Investigating microbial homeostasis in shift cytokine storm immunosuppression may elucidate between metabolites, dysfunction, organ injury, providing foundation for targeted therapies drug development. Traditional Chinese Medicine (TCM) has demonstrated significant advantages mitigating sepsis-associated storms modulating gut homeostasis, offering promising strategy developing highly effective less toxic monomeric compounds. Elucidating interactions within immune-inflammation-microbiota network sepsis paves way biomarker-driven personalized therapeutic approaches.

Language: Английский

Extracellular Vesicle ASC: A Novel Mediator for Lung–Brain Axis in Preterm Brain Injury DOI

N. Starke,

Naga Venkata Divya Challa,

Huijun Yuan

et al.

American Journal of Respiratory Cell and Molecular Biology, Journal Year: 2024, Volume and Issue: 71(4), P. 464 - 480

Published: July 3, 2024

Bronchopulmonary dysplasia (BPD) and neurodevelopmental impairment are among the most common morbidities affecting preterm infants. Although BPD is a predictor of poor outcomes, it currently uncertain how contributes to brain injury in Extracellular vesicles (EVs) involved interorgan communication diverse pathological processes. ASC (apoptosis-associated speck-like protein containing caspase recruitment domain) pivotal inflammasome assembly activation inflammatory response. We assessed expression profiles alveolar macrophage (AM) markers CD11b, CD11c, CD206 as well EVs isolated from plasma infants at risk for 1 week age. found that on higher fraction inspired oxygen therapy (HO

Language: Английский

Citations

4
The new pattern for dual NOTCH pathway involving nuclear transcription and mitochondrial regulation supports therapeutic mechanism of 4-butyl benzophenone derivatives against SIRS DOI
Jiayu Song, Dan Peng, Yu Peng

et al.

Free Radical Biology and Medicine, Journal Year: 2024, Volume and Issue: 223, P. 306 - 324

Published: Aug. 10, 2024

Language: Английский

Citations

4
Macrophages in sepsis-induced acute lung injury: exosomal modulation and therapeutic potential DOI Creative Commons

Kangzheng Lv,

Qun Liang

Frontiers in Immunology, Journal Year: 2025, Volume and Issue: 15

Published: Jan. 7, 2025

Sepsis-induced acute lung injury (ALI) remains a leading cause of mortality in critically ill patients. Macrophages, key modulators immune responses, play dual role both promoting and resolving inflammation. Exosomes, small extracellular vesicles released by various cells, carry bioactive molecules that influence macrophage polarization responses. Emerging researchers have identified exosomes as crucial mediators modulate activity during sepsis-induced ALI. This review explores the modulating functions, focusing on cellular interactions within microenvironment their potential therapeutic targets. It highlights regulation macrophages derived from pathogenic germs, neutrophils, alveolar epithelial mesenchymal stromal cells. By understanding these mechanisms, it aims to uncover innovative strategies for

Language: Английский

Citations

0
Efficacy of caerulomycin A in modulating macrophage polarization and cytokine response in a murine model of lipopolysaccharide-induced sepsis DOI Creative Commons
Jun Zhang,

Shiyue Tang

European journal of medical research, Journal Year: 2025, Volume and Issue: 30(1)

Published: Jan. 10, 2025

Sepsis is characterized by an excessive immune response. Modulation of the response, particularly macrophage polarization, may provide therapeutic benefit. The effects Caerulomycin A (caeA), a known STAT1 phosphorylation inhibitor, on polarization and inflammatory markers were explored using lipopolysaccharide (LPS)-induced sepsis mouse model. model was established in C57BL/6 mice induced intraperitoneal injection LPS, survival rate observed after treatment with different doses caeA to determine optimal dose. For in-vitro assays RAW264.7 cell line, concentration that non-toxic screened MTT assay, followed analyses qRT-PCR, ELISA, Western blot flow cytometry for M1/M2 type (CD86, NOS2, CD206, ARG1) factors (IL-1β, IL-6, TNF-α, IL-4, IL-10) expression. In addition, levels STAT6 JAK–STAT signaling pathway detected. results in-vivo experiments showed (20 mg/kg) significantly increased LPS-induced septic decreased expression M1-type (CD86 NOS2) pro-inflammatory cytokines TNF-α) while increasing M2-type (CD206 anti-inflammatory (IL-4 experiments, 20 μM effectively inhibited macrophages without affecting activity cells, yet enhanced level STAT6, as detected blot. CaeA modulates attenuates response mice, possibly pathway. These findings support further exploration potential agent sepsis.

Language: Английский

Citations

0
Increased pro-SFTPB in HDL promotes the pro-inflammatory transition of HDL and represents a sign of poor prognosis in ARDS patients DOI Creative Commons

Liu Yang,

Zhuo Xu,

Zhenyan Wang

et al.

Journal of Translational Medicine, Journal Year: 2025, Volume and Issue: 23(1)

Published: Jan. 16, 2025

Language: Английский

Citations

0
Glabridin Suppresses Macrophage Activation by Lipoteichoic Acid In Vitro: The Crucial Role of MAPKs-IL-1β-iNOS Axis Signals in Peritoneal and Alveolar Macrophages DOI Creative Commons
Shaw‐Min Hou, Chunming Yang, Wei‐Chieh Huang

et al.

Biomolecules, Journal Year: 2025, Volume and Issue: 15(2), P. 174 - 174

Published: Jan. 24, 2025

Inflammation, a fundamental response to infection and injury, involves interactions among immune cells signaling molecules. Dysregulated inflammation contributes diseases such as autoimmune disorders cancer. Interleukin-1 beta (IL-1β), produced by macrophages in lipoteichoic acid (LTA) from Gram-positive bacteria, is key inflammatory mediator. Glabridin (GBD), bioactive compound licorice root, exhibits anti-inflammatory properties. This study investigates GBD’s effects on LTA-induced proinflammatory RAW 264.7 murine alveolar macrophages, MH-S, focusing IL-1β expression pathways. Cell viability assays confirmed that 20 μM GBD was non-cytotoxic. Confocal microscopy quantitative PCR showed significantly reduced fluorescence intensity, mRNA, protein levels. also inhibited inducible nitric oxide synthase (iNOS) (NO) production. Further analysis revealed suppressed NF-κB p65 nuclear translocation selectively modulated MAPK pathway activation reducing JNK p38 phosphorylation without affecting ERK. Studies using specific inhibitors demonstrated production reduction mechanistically linked inhibition. These findings highlight potential therapeutic agent for through its ability modulate critical mediators

Language: Английский

Citations

0
Comprehensive single-cell analysis deciphered the immunoregulatory mechanism of TPPU in alleviating sepsis-related acute liver injury DOI Creative Commons

Juan Li,

Mengjuan Xuan,

Yang Li

et al.

Journal of Advanced Research, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 1, 2025

Language: Английский

Citations

0
TREM2 improves coagulopathy and lung inflammation in sepsis through the AKT-mTOR pathway DOI
Chen Zhou,

Chenglong Liang,

Rongrong Zhang

et al.

International Immunopharmacology, Journal Year: 2025, Volume and Issue: 150, P. 114330 - 114330

Published: Feb. 18, 2025

Language: Английский

Citations

0
HNF4A mitigates sepsis-associated lung injury by upregulating NCOR2/GR/STAB1 axis and promoting macrophage polarization towards M2 phenotype DOI Creative Commons
Yuhang Yang, Ri Wen, Xinmei Huang

et al.

Cell Death and Disease, Journal Year: 2025, Volume and Issue: 16(1)

Published: Feb. 20, 2025

Sepsis can trigger systemic inflammation and lead to detrimental effects on several organs, with particular emphasis the lungs. In sepsis-associated lung injury, macrophages assume a pivotal role, as their overactivation could facilitate secretion of inflammatory factors imbalance polarization. Hepatocyte nuclear factor 4 alpha (HNF4A) has been reported its potential involvement in regulation response macrophage This study discusses role mechanism HNF4A sepsis-induced damage. exhibits decrease expression by analyzing differentially expressed genes lungs septic mice from Gene Expression Omnibus dataset GSE15379. Then, we established mouse sepsis model through cecal ligation puncture method observed that was reduced both tissues alveolar macrophages. To evaluate function HNF4A, overexpressed mediated adenovirus vectors, which were injected into mice. We found overexpression resulted higher survival rate an amelioration pulmonary Meanwhile, mitigated infiltration cells impeded M1 polarization but facilitated M2 or lavage fluid. vitro, treated bone marrow-derived interleukin-4. Consistent results obtained promoted Mechanistically, transcriptionally regulate receptor coactivator 2 (NCOA2) binding promoter region. NCOA2 interacted glucocorticoid (GR). Stabilin 1 (STAB1) selected possible target transcriptome sequencing analysis. Functional experiments confirmed STAB1 downstream HNF4A/NCOA2/GR axis. Overall, this research investigated impact injury sepsis. It is suggested one regulatory mechanisms involved association may be NCOR2/GR/STAB1

Language: Английский

Citations

0
Identification of Subtypes and Diagnostic Markers Related to Necroptosis in Sepsis DOI Creative Commons

Sen Peng,

Ning Meng,

Xia Xie

et al.

Applied Biochemistry and Biotechnology, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 26, 2025

Language: Английский

Citations

0