The impact of glucose metabolism on inflammatory processes in sepsis-induced acute lung injury DOI Creative Commons

Shilei Cheng,

Yufei Li, Xiaoliang Sun

et al.

Frontiers in Immunology, Journal Year: 2024, Volume and Issue: 15

Published: Dec. 6, 2024

Acute lung injury (ALI) is a prevalent and critical complication of sepsis, marked by high incidence mortality rates, with its pathogenesis still not being fully elucidated. Recent research has revealed significant correlation between the metabolic reprogramming glucose sepsis-associated ALI (S-ALI). Throughout course S-ALI, immune cells, including macrophages dendritic undergo shifts to accommodate intricate demands function that emerge as sepsis advances. Indeed, in S-ALI serves double-edged sword, fueling inflammatory responses initial stages subsequently initiating anti-inflammatory disease evolves. In this review, we delineate current progress concerning pathogenic mechanisms linked focus on pertinent cells implicated. We encapsulate impact onset, progression, prognosis S-ALI. Ultimately, examining key regulatory factors within intermediates enzymes, have identified potential therapeutic targets reprogramming, striving tackle inherent challenges diagnosing treating Severe Lung Injury (S-ALI) greater efficacy.

Language: Английский

The METTL14‐YTHDF1‐SAP30 Axis Promotes Glycolysis and Oxaliplatin Resistance in Colorectal Adenocarcinoma via m6A Modification DOI
Haoran Zhang,

Xi Wu,

Jinlin Nie

et al.

Journal of Gastroenterology and Hepatology, Journal Year: 2025, Volume and Issue: unknown

Published: April 27, 2025

ABSTRACT Colorectal cancer (CRC) is a prevalent with poor prognosis, especially in advanced metastatic stages. This study identifies SAP30 as significantly upregulated gene COAD, linking high expression to reduced overall survival. Experimental validation revealed elevated levels CRC cell lines (SW480, RKO, HT29, and HCT15), the highest oxaliplatin‐resistant sublines (HT29‐OxR HCT15‐OxR). knockdown cells glycolytic activity, glucose consumption, enzyme (LDHA, HK1, HK2, GLUT1, GLUT4), while overexpression enhanced glycolysis, partially reversed by GLUT1 inhibitor WZB117. also promoted proliferation, inhibited apoptosis, migration invasion resistant cells. Mechanistically, METTL14, an m6A methyltransferase, upregulates mRNA via modification, stabilized reader protein YTHDF1. METTL14‐YTHDF1‐SAP30 axis sustains expression, promoting glycolysis oxaliplatin resistance. In vivo studies confirmed that knockout impairs tumor growth reduces proliferation markers. highlights chemoresistance CRC, suggesting potential target overcome resistance improve patient outcomes.

Language: Английский

Citations

0
DeepKlapred: A deep learning framework for identifying protein lysine lactylation sites via multi-view feature fusion DOI

Jiahui Guan,

Peilin Xie,

Danhong Dong

et al.

International Journal of Biological Macromolecules, Journal Year: 2024, Volume and Issue: unknown, P. 137668 - 137668

Published: Nov. 1, 2024

Language: Английский

Citations

2
Lactate promotes H3K18 lactylation in human neuroectoderm differentiation DOI Creative Commons
Yu Wu, Yumeng Wang, Yu Dong

et al.

Cellular and Molecular Life Sciences, Journal Year: 2024, Volume and Issue: 81(1)

Published: Nov. 20, 2024

In mammals, early embryonic gastrulation process is high energy demanding. Previous studies showed that, unlike endoderm and mesoderm cells, neuroectoderm differentiated from human stem cells relied on aerobic glycolysis as the major metabolic process, which generates lactate final product. Here we explored function of intracellular during differentiation. Our results revealed that level was elevated in exogenous could further promote hESCs differentiation towards neuroectoderm. Changing levels by sodium or LDHA inhibitors had no obvious effect BMP WNT/β-catenin signaling Notably, histone lactylation, especially H3K18 lactylation significant upregulated this process. We performed CUT&Tag experiments H3K18la highly enriched at gene promoter regions. By analyzing data RNA-seq experiments, identified four genes, including PAX6, were transcriptionally A modification site PAX6 verified also rescue after shPAX6 inhibition.

Language: Английский

Citations

2
TGF-β2 enhances glycolysis in chondrocytes via TβRI/p-Smad3 signaling pathway DOI

Jieya Wei,

Siqun Xu,

Yang Liu

et al.

Biochimica et Biophysica Acta (BBA) - Molecular Cell Research, Journal Year: 2024, Volume and Issue: 1871(7), P. 119788 - 119788

Published: June 13, 2024

Language: Английский

Citations

1
The impact of glucose metabolism on inflammatory processes in sepsis-induced acute lung injury DOI Creative Commons

Shilei Cheng,

Yufei Li, Xiaoliang Sun

et al.

Frontiers in Immunology, Journal Year: 2024, Volume and Issue: 15

Published: Dec. 6, 2024

Acute lung injury (ALI) is a prevalent and critical complication of sepsis, marked by high incidence mortality rates, with its pathogenesis still not being fully elucidated. Recent research has revealed significant correlation between the metabolic reprogramming glucose sepsis-associated ALI (S-ALI). Throughout course S-ALI, immune cells, including macrophages dendritic undergo shifts to accommodate intricate demands function that emerge as sepsis advances. Indeed, in S-ALI serves double-edged sword, fueling inflammatory responses initial stages subsequently initiating anti-inflammatory disease evolves. In this review, we delineate current progress concerning pathogenic mechanisms linked focus on pertinent cells implicated. We encapsulate impact onset, progression, prognosis S-ALI. Ultimately, examining key regulatory factors within intermediates enzymes, have identified potential therapeutic targets reprogramming, striving tackle inherent challenges diagnosing treating Severe Lung Injury (S-ALI) greater efficacy.

Language: Английский

Citations

1