Sporadic inclusion body myositis-derived myotube culture revealed muscle cell-autonomous expression profiles DOI Creative Commons
Naoki Suzuki, Makoto Kanzaki, Masashi Koide

et al.

Research Square (Research Square), Journal Year: 2023, Volume and Issue: unknown

Published: Oct. 13, 2023

Abstract Sporadic inclusion body myositis (sIBM) is a muscle disease in older people and characterized by inflammatory cell invasion into intact fibers rimmed vacuoles. The pathomechanism of sIBM not fully elucidated yet, controversy exists as to whether primary autoimmune or degenerative with secondary inflammation. Previously, we established method collecting CD56-positive myoblasts from human skeletal biopsy samples. We hypothesized that the derived these patients are useful see cell-autonomous sIBM. With resources, were differentiated myotubes, expression profiles pathology analyzed. Myoblasts three cases six controls myotubes. In RNA-sequencing analysis “myotube” samples, 104 differentially expressed genes (DEGs) found be significantly upregulated more than twofold sIBM, 13 DEGs downregulated less twofold. For comparative was conducted determine extent which “biopsy” samples differed. Fifty-three extracted 32 (60%) had opposite directions change (e.g., increased vs decreased myotube). Apolipoprotein E (apoE) transmembrane protein 8C (TMEM8C) commonly biopsies myotubes ApoE myogenin levels Given enrichment also captured changes contraction development, triggering atrophy signaling abnormal differentiation via TMEM8C may involved pathogenesis presence suggests formed sIBM-derived revealed existence degeneration catalog will an important resource for future studies on focusing degeneration.

Language: Английский

Novel insights into the role of ubiquitination in osteoarthritis DOI

Yuzhe Lin,

Shide Jiang,

Jingyue Su

et al.

International Immunopharmacology, Journal Year: 2024, Volume and Issue: 132, P. 112026 - 112026

Published: April 8, 2024

Language: Английский

Citations

6
Analysis of cellular senescence-related genes in calcified aortic valve disease and the potential therapeutic role of β-Carotene DOI Creative Commons
Yijing Tao, Chengjie Gao, Juan Wang

et al.

PLoS ONE, Journal Year: 2025, Volume and Issue: 20(3), P. e0318574 - e0318574

Published: March 10, 2025

Objective Calcific aortic valve disease (CAVD) is a progressive, age-related degenerative characterized by the accumulation of calcium deposits in valve. We aim to screen key genes associated with cellular senescence (CS) CAVD. Methods The GSE12644 and GSE51472 datasets from GEO database was utilized this study, differentially expressed (DEGs) were identified using “ limma ” R package. CS-related DEGs (CS-DEGs) determined through CellAge database. Gene Ontology (GO) Kyoto Encyclopedia Genes Genomes (KEGG) enrichment analyses performed on CS-DEGs. A protein–protein interaction (PPI) network constructed STRING cytoHubba plug-in Cytoscape used identify hub genes. noncoding-RNA-mRNA regulatory established. DSigDB drugs potentially be useful for treating Results total 16 CS-DEGs identified. These primarily collagen metabolic process, catabolic process external side plasma membrane. 10 as regulators CAVD: LPAR1, PTPN6, CD28, ID1, MEIS2, FGFR3, KDR, MMP7, AR, HIF1A. Noncoding RNA-mRNA indicated that may regulated noncoding RNAs. β-Carotene, naturally occurring carotenoid antioxidant properties, potential therapeutic agents interacting MMP9, CTSB. Conclusion This study provides insights into pathways related CAVD (MMP9, CTSB) highlights role β-Carotene treatment

Language: Английский

Citations

0
Therapeutic prospects and potential mechanisms of Prdx6: as a novel target in musculoskeletal disorders DOI Creative Commons
Hongbin Sun, Chao Xu,

Zhilin Xiong

et al.

Frontiers in Physiology, Journal Year: 2025, Volume and Issue: 16

Published: April 17, 2025

With the global population aging, musculoskeletal disorders (MSDs) have posed significant physical and psychological health challenges for patients as well a substantial economic burden on society. The advancements in conservative surgical interventions MSDs been remarkable recent years; however, current treatment modalities still fall short of meeting optimal requirements patients. Recently, peroxiredoxin 6 (Prdx6) has gained considerable attention from researchers due to its antioxidative, anti-inflammatory, anti-apoptotic properties. It found that Prdx6 is involved multiple system diseases, including MSDs; exact role lacking. This study aimed summarize structure, regulatory mechanism, potential function Prdx6. These findings may demonstrate novel target inhibiting advancement MSDs.

Language: Английский

Citations

0
YAP/TAZ-mediated nuclear membrane rupture in promoting senescence of skeletal muscle associated with COPD DOI Creative Commons

Ge Gong,

Shuping Shen,

Shaoran Shen

et al.

Respiratory Research, Journal Year: 2025, Volume and Issue: 26(1)

Published: March 12, 2025

Patients with chronic obstructive pulmonary disease (COPD) often develop complications associated sarcopenia; however, the underlying mechanisms remain unclear. Through a combination of in vitro and vivo experiments, as well bioinformatics analysis, our study identified YAP/TAZ key regulator aging phenotype skeletal muscle COPD patients. In affected by cigarette smoke-induced COPD, we observed significant reductions levels, alongside markers indicative dysfunction. Notably, overexpression significantly improved these conditions. Our results suggest novel mechanism whereby maintenance activity interacts ACTR2 to preserve nuclear membrane integrity reduce cytoplasmic dsDNA thereby attenuating STING activation cellular senescence. Additionally, found that YAP is involved transcriptional regulation promoter region. Overall, preserving may help prevent representing new strategy for intervening COPD-related sarcopenia. Not applicable.

Language: Английский

Citations

0
Mitochondrial dysfunction, a weakest link of network of aging, relation to innate intramitochondrial immunity of DNA recognition receptors DOI
Dun‐Xian Tan

Mitochondrion, Journal Year: 2024, Volume and Issue: 76, P. 101886 - 101886

Published: April 24, 2024

Language: Английский

Citations

2
The role of YAP/TAZ on joint and arthritis DOI
Mingcheng Lu, Mengqi Zhu, Zuping Wu

et al.

The FASEB Journal, Journal Year: 2024, Volume and Issue: 38(10)

Published: May 16, 2024

Osteoarthritis (OA) and rheumatoid arthritis (RA) are two common forms of with undefined etiology pathogenesis. Yes-associated protein (YAP) its homolog transcriptional coactivator PDZ-binding motif (TAZ), which act as sensors for cellular mechanical inflammatory cues, have been identified crucial players in the regulation joint homeostasis. Current studies also reveal a significant association between YAP/TAZ pathogenesis OA RA. The objective this review is to elucidate impact on different tissues provide inspiration further studying potential therapeutic implications arthritis. Databases, such PubMed, Cochran Library, Embase, were searched all available during past decades, keywords "YAP," "TAZ," "OA," "RA."

Language: Английский

Citations

2
Deletion of absent in melanoma-2 (AIM2) drives bone marrow adipogenesis and impairs bone microarchitecture DOI
Zhenwei Gong, Manisha Dixit, Zhiming He

et al.

GeroScience, Journal Year: 2024, Volume and Issue: unknown

Published: Sept. 26, 2024

Language: Английский

Citations

1
The cross-talk between the cGAS-STING signaling pathway and chronic inflammation in the development of musculoskeletal disorders DOI
Alexander Kalinkovich, Gregory Livshits

Ageing Research Reviews, Journal Year: 2024, Volume and Issue: unknown, P. 102602 - 102602

Published: Nov. 1, 2024

Language: Английский

Citations

1
Acacetin targets STING to alleviate the destabilization of the medial meniscus-induced osteoarthritis in mice DOI

Dingjun Xu,

Linjie Zhang,

Chenyu Song

et al.

Naunyn-Schmiedeberg s Archives of Pharmacology, Journal Year: 2024, Volume and Issue: 397(11), P. 8863 - 8878

Published: June 10, 2024

Language: Английский

Citations

0
Sporadic inclusion body myositis-derived myotube culture revealed muscle cell-autonomous expression profiles DOI Creative Commons
Naoki Suzuki, Makoto Kanzaki, Masashi Koide

et al.

Research Square (Research Square), Journal Year: 2023, Volume and Issue: unknown

Published: Oct. 13, 2023

Abstract Sporadic inclusion body myositis (sIBM) is a muscle disease in older people and characterized by inflammatory cell invasion into intact fibers rimmed vacuoles. The pathomechanism of sIBM not fully elucidated yet, controversy exists as to whether primary autoimmune or degenerative with secondary inflammation. Previously, we established method collecting CD56-positive myoblasts from human skeletal biopsy samples. We hypothesized that the derived these patients are useful see cell-autonomous sIBM. With resources, were differentiated myotubes, expression profiles pathology analyzed. Myoblasts three cases six controls myotubes. In RNA-sequencing analysis “myotube” samples, 104 differentially expressed genes (DEGs) found be significantly upregulated more than twofold sIBM, 13 DEGs downregulated less twofold. For comparative was conducted determine extent which “biopsy” samples differed. Fifty-three extracted 32 (60%) had opposite directions change (e.g., increased vs decreased myotube). Apolipoprotein E (apoE) transmembrane protein 8C (TMEM8C) commonly biopsies myotubes ApoE myogenin levels Given enrichment also captured changes contraction development, triggering atrophy signaling abnormal differentiation via TMEM8C may involved pathogenesis presence suggests formed sIBM-derived revealed existence degeneration catalog will an important resource for future studies on focusing degeneration.

Language: Английский

Citations

0