International Journal of Molecular Sciences,
Journal Year:
2023,
Volume and Issue:
24(24), P. 17299 - 17299
Published: Dec. 9, 2023
As
the
prevalence
of
juvenile-onset
obesity
rises
globally,
multitude
related
health
consequences
gain
significant
importance.
In
this
context,
is
associated
with
impaired
cutaneous
wound
healing.
experimental
settings,
mice
are
most
frequently
used
model
for
investigating
effect
high-fat
diet
(HFD)
chow
on
healing
in
wild-type
or
genetically
manipulated
animals,
e.g.,
diabetic
ob/ob
and
db/db
mice.
However,
these
studies
have
mainly
been
performed
adult
animals.
Thus,
present
study,
we
introduced
a
mouse
juvenile
onset
obesity.
We
exposed
4-week-old
to
an
investigational
feeding
period
9
weeks
HFD
compared
regular
(RD).
At
age
13
weeks,
excisional
incisional
wounding
measured
rate.
Wound
was
examined
by
serial
photographs
daily
size
measurements
wounds.
Histology
from
wounds
quantify
granulation
tissue
(thickness,
quality)
angiogenesis
(number
blood
vessels
per
mm2).
The
expression
extracellular
matrix
proteins
(collagen
types
I/III/IV,
fibronectin
1,
elastin),
inflammatory
cytokines
(MIF,
MIF-2,
IL-6,
TNF-α),
myofibroblast
differentiation
(α-SMA)
macrophage
polarization
(CD11c,
CD301b)
were
evaluated
RT-qPCR
immunohistochemistry.
There
marked
delay
closure
group
decrease
quality
thickness.
Additionally,
TNF-α)
significantly
up-regulated
HFD-
when
RD-fed
at
day
3.
By
contrast,
MIF-2
vessel
reduced
starting
1.
No
changes
observed
polarization,
collagen
expression,
levels
TGF-β1
PDGF-A.
Our
findings
support
that
early
exposition
resulted
repair
mechanisms,
which
may
be
as
murine
obesity-related
future.
Nutrients,
Journal Year:
2025,
Volume and Issue:
17(2), P. 266 - 266
Published: Jan. 13, 2025
Background:
This
study
investigated
the
wound-healing
potential
of
hispolon,
a
polyphenolic
pigment
derived
from
medicinal
mushrooms,
under
diabetic
conditions
using
both
in
vitro
and
vivo
models.
Methods:
In
assays,
L929
fibroblast
cells
exposed
to
high
glucose
(33
mmol/L)
were
treated
with
hispolon
at
concentrations
2.5,
5,
7.5,
or
10
μmol/L.
streptozotocin-induced
rats
excision
wounds
received
daily
topical
applications
0.2
g
5%
(w/w)
ointment.
Results:
Hispolon
improved
cell
viability;
suppressed
oxidative
stress
by
reducing
reactive
oxygen
species,
lipid
peroxidation,
DNA
damage;
restored
reduced
glutathione/oxidized
glutathione
ratio.
The
scratch
assay
demonstrated
that
μmol/L
enhanced
migration
impaired
high-glucose
conditions.
Treatment
ointment
accelerated
wound
contraction,
epithelialization
time,
tissue
regeneration
an
efficacy
comparable
Fespixon®
cream,
as
shown
histological
findings
increased
activity,
collagen
deposition,
capillary
growth.
also
modulated
macrophage
polarization
M1
markers
enhancing
M2
markers.
rat
dead-space-wound
model,
levels
pro-inflammatory
cytokines,
those
anti-inflammatory
cytokines
growth
factors,
stimulated
Type
I
III
synthesis,
effectively
promoting
healing.
incisional
wounds,
wound-breaking
strength,
showing
results
cream.
Safety
assessments
confirmed
showed
no
acute
dermal
toxicity.
These
underscore
hispolon’s
promising
candidate
for
management
mitigating
stress,
regeneration,
accelerating
Matrix Biology,
Journal Year:
2024,
Volume and Issue:
128, P. 31 - 38
Published: Feb. 28, 2024
The
largest
mammalian
organ,
skin,
consisting
of
a
dermal
connective
tissue
layer
that
underlies
and
supports
the
epidermis,
acts
as
protective
barrier
excludes
external
pathogens
disseminates
sensory
signals
emanating
from
local
microenvironment.
Dermal
is
comprised
collagen-rich
extracellular
matrix
(ECM)
produced
by
fibroblasts
resident
within
dermis.
When
wounded,
repair
program
induced
whereby
fibroblasts,
in
response
to
alterations
microenvironment,
produce
new
ECM
components,
resulting
formation
scar.
Failure
terminate
normal
causes
fibrotic
conditions
including:
hypertrophic
scars,
keloids,
systemic
autoimmune
disease
scleroderma
(systemic
sclerosis,
SSc).
Histological
single-cell
RNA
sequencing
(scRNAseq)
studies
have
revealed
are
heterogeneous
highly
plastic.
Understanding
how
this
diversity
contributes
homeostasis,
wounding,
fibrosis,
cancer
may
ultimately
result
novel
anti-fibrotic
therapies
personalized
medicine.
This
review
summarizes
supporting
concept.
Life,
Journal Year:
2023,
Volume and Issue:
14(1), P. 40 - 40
Published: Dec. 25, 2023
The
skin's
recognised
functions
may
undergo
physiological
alterations
due
to
ageing,
manifesting
as
varying
degrees
of
facial
wrinkles,
diminished
tautness,
density,
and
volume.
Additionally,
these
can
be
disrupted
(patho)physiologically
through
various
physical
chemical
injuries,
including
surgical
trauma,
accidents,
or
chronic
conditions
like
ulcers
associated
with
diabetes
mellitus,
venous
insufficiency,
obesity.
Advancements
in
therapeutic
interventions
that
boost
the
innate
regenerative
abilities
could
significantly
enhance
patient
care
protocols.
application
Platelet-Rich
Plasma
(PRP)
is
widely
recognized
for
its
aesthetic
functional
benefits
skin.
Yet,
endorsement
PRP's
advantages
often
borders
on
dogmatic,
efficacy
commonly
ascribed
solely
activation
fibroblasts
by
factors
contained
within
platelet
granules.
PRP
therapy
a
cornerstone
medicine
which
involves
autologous
delivery
conditioned
plasma
enriched
platelets.
This
achieved
centrifugation,
removing
erythrocytes
while
retaining
platelets
their
Despite
widespread
use,
precise
sequences
cellular
activation,
specific
players,
molecular
machinery
drive
PRP-facilitated
healing
are
still
enigmatic.
There
paucity
definitive
robust
studies
elucidating
mechanisms.
In
recent
years,
telocytes
(TCs)-a
unique
dermal
cell
population-have
shown
promising
potential
tissue
regeneration
organs,
dermis.
TCs'
participation
neo-angiogenesis,
akin
attributed
PRP,
role
remodelling
repair
processes
interstitia
several
organs
(including
dermis),
offer
intriguing
insights.
Their
contribute
to,
possibly
orchestrate,
skin
process
following
treatment
has
elicited
considerable
interest.
Therefore,
pursuing
comprehensive
understanding
mechanisms
at
work,
particularly
those
involving
TCs,
temporal
involvement
structural
recovery
injury,
interconnected
biological
events
wound
represents
compelling
field
study.
Scientific Reports,
Journal Year:
2025,
Volume and Issue:
15(1)
Published: Jan. 7, 2025
The
aim
of
this
study
was
to
investigate
the
effect
dental
pulp
stem
cell-derived
exosomes
(DPSCs-Exos)
on
biological
behaviour
fibroblasts,
particularly
keloid
fibroblasts
(KFs)
and
normal
skin
(NFs),
with
a
view
providing
new
insights
into
cellular
regenerative
medicine.
We
obtained
DPSCs-Exos
by
ultracentrifugation
co-cultured
it
KFs
NFs.
detected
its
cell
proliferation
using
CCK-8
assay;
migration
ability
scratch
Transwell
assays;
extracellular
matrix
synthesis
hydroxyproline
content
expression
levels
genes
associated
fibrosis
PCR
proteins
related
in
cells
Western
Blot
method.
able
be
taken
up
after
addition
culture
medium
affected
behavior
NFs
KFs.
promoted
NFs,
inhibited
In
addition,
inhibit
fibrosis-related
This
highlights
role
regulating
for
future
applications
field
cell-free
Lymphatics,
Journal Year:
2025,
Volume and Issue:
3(1), P. 7 - 7
Published: March 6, 2025
The
impaired
repair
of
lymphatic
vessels
after
tissue
damage
is
an
etiological
hallmark
lymphedema.
Previously,
we
demonstrated
that
recanalization
the
popliteal
lymph
node
extirpation
was
delayed
in
Gata2
heterozygous
mice.
This
vessel
mice
mitigated
by
administrating
atelocollagen
or
crossing
with
Gata3
deletion
To
clarify
potential
involvement
heterozygosity
collagen
gene
expression
within
subdermal
tissue,
conducted
RNAseq
analysis
and
found
273
genes
up
522
down
mice,
these
were
categorized
as
extracellular
matrix-related
GO
analysis.
We
also
Col6a1,
a2,
a3,
which
compose
type
VI
collagen,
underwent
a
transient
but
significant
upregulation
during
process.
Histological
revealed
structure
exhibited
thinner
fiber
deficient
These
findings
suggest
altered
pattern
contributed
to
enhanced
might
play
role
shaping
maintaining
subcutaneous
microenvironment.
