APOPTOSIS, Journal Year: 2024, Volume and Issue: unknown
Published: Dec. 26, 2024
Language: Английский
Frontiers in Immunology, Journal Year: 2023, Volume and Issue: 14
Published: Dec. 20, 2023
Introduction Tobacco smoking generates airway inflammation in chronic obstructive pulmonary disease (COPD), and its involvement the development of lung cancer is still among leading causes early death. Therefore, we aimed to have a better understanding disbalance immunoregulation inflammatory conditions smoker subjects with stable COPD (stCOPD), exacerbating (exCOPD), or non-small cell (NSCLC). Methods Smoker controls without illness were recruited as controls. Through extensive mapping single cells, surface receptor quantification was achieved by single-cell mass cytometry (CyTOF) 29 antibodies. The CyTOF characterized 14 main immune subsets such CD4+, CD8+, CD4+/CD8+, CD4−/CD8−, γ/δ T cells other CD4+ CD8+ NKT NK B plasmablasts, monocytes, CD11c dim , mDCs, pDCs. central memory (CM) (CD4+/CD45RA−/CD45RO+/CD197+) effector (EM) (CD4+/CD45RA−/CD45RO+/CD197−) FACS-sorted for RNA-Seq analysis. Plasma samples assayed Luminex MAGPIX ® quantitative measurement 17 soluble immuno-oncology mediators (BTLA, CD28, CD80, CD27, CD40, CD86, CTLA-4, GITR, GITRL, HVEM, ICOS, LAG-3, PD-1, PD-L1, PD-L2, TIM-3, TLR-2) four studied groups. Results Our focus on T-cell-dependent differences NSCLC, where peripheral showed significant reduction exCOPD CM elevation NSCLC. transcriptome analysis delineated perfect correlation differentially expressed genes between NSCLC-derived EM cells. revealed disease-associated phenotype blood stCOPD, exCOPD, NSCLC patients. Discussion applied cytometry, whole profiling plasma provided complex data that may contribute homeostasis generated sustained tobacco
Language: Английский
Citations
14
United European Gastroenterology Journal, Journal Year: 2024, Volume and Issue: 12(8), P. 1102 - 1113
Published: Aug. 6, 2024
Abstract Background Gelsectan ® is a formulation of xyloglucan (XG), pea protein, grape seed extract (PPGS) and xylo‐oligosaccharides (XOS). Our aim was to examine the effect on rectal sensitivity in an animal model, abdominal pain irritable bowel syndrome with diarrhoea (IBS‐D) subjects intestinal permeability both conditions. Methods Animals: Wistar rats received gavage XOS, XG + PPGS or as single dose for 7 days before partial restraint stress (PRS). Visceromotor response distension total gut paracellular 51 Cr‐EDTA were assessed. Humans: IBS‐D control patients involved. After initial colonoscopy biopsy sampling Gelsectan® administered 12 weeks. Stool count scores documented. treatment, repeated. The samples measured Ussing‐chambers. Adherent mucus layer, Muc‐2 expression well TNFα, Interferon IFNγ evaluated by histology/immunohistochemistry ELISA assays, respectively. Results Animal studies: In rats, PRS significantly increased visceromotor permeability. Single administration XOS failed reverse PRS, but oral treatment reversed PRS‐induced hypersensitivity hyperpermeability. Human reduced pain. Intestinal elevated compared controls, restored ascendent colon. Periodic acid–Schiff‐stained layer thinner segments, thickness proportion positive cells not affected treatment. tissue level sigmoid colon shows modest mucosal inflammation IBS‐D. Conclusions prevented human hyperpermeability rat studies These effects involve restoration Based this translational study, can be considered effective therapy symptoms.
Language: Английский
Citations
5
Frontiers in Immunology, Journal Year: 2024, Volume and Issue: 15
Published: May 7, 2024
Objectives Cell surface glycosylation can influence protein-protein interactions with particular relevance to changes in core fucosylation and terminal sialylation. Glycans are ligands for immune regulatory lectin families like galectins (Gals) or sialic acid immunoglobulin-like lectins (Siglecs). This study delves into the glycan alterations within subsets of systemic lupus erythematosus (SLE). Methods Evaluation binding affinities Galectin-1, Galectin-3, Siglec-1, Aleuria aurantia (AAL, recognizing fucosylation), Sambucus nigra agglutinin (SNA, specific α-2,6-sialylation) was conducted on various peripheral blood mononuclear cells (PBMCs) from control SLE subjects. Lectin measured by multi-parameter flow cytometry 18 manually gated T-cells, NK-cells, NKT-cells, B-cells, monocytes unstimulated resting state also after 3-day activation. Stimulated pre-gated populations were subsequently clustered FlowSOM algorithm based activation markers, CD25 HLA-DR. Results Elevated AAL, SNA + /CD25 - ratio certain stimulated T-cell correlated Disease Activity Index 2000 (SLEDAI-2K) scores. The significantly increased frequencies activated AAL low Siglec-1 NK metaclusters SLEDAI-2K indices. In SLE, double negative NKTs displayed lower ratio, negatively correlating disease activity. enhanced plasmablasts positively Conclusion Alterations correlate severity, which might represent potential implications pathogenesis SLE.
Language: Английский
Citations
4
International Journal of Obesity, Journal Year: 2024, Volume and Issue: 48(11), P. 1568 - 1576
Published: July 14, 2024
Abstract Background Obesity is a growing problem worldwide and major risk factor for many chronic diseases. The accumulation of adipose tissue leads to the release significant amounts pro-inflammatory cytokines adipokines, resulting in low-grade systemic inflammation. However, mechanisms behind development obesity-related diseases are not fully understood. Therefore, our study aimed investigate pathological changes inflammatory processes at level individual organs two different diet-induced mouse obesity models. Methods Male C57BL6/J mice were fed by high-fat diet (HFD), high-fat/high-fructose (HFD + FR) or normal chow 21 weeks starting 3 months age ( n = 15 animals/group). Insulin resistance was tested oral glucose tolerance test. Pathological investigated on hematoxylin – eosin-stained liver brown sections. gene expression levels adipokines analyzed qPCR tissues, whereas serum protein concentrations determined multiplex immunoassays. Immunophenotyping isolated blood, bone marrow spleen cells performed single-cell mass cytometry. Results Weight gain, intolerance hepatic steatosis more severe HFD FR group than control groups. This accompanied higher inflammation, as indicated increased genes visceral white TNFα level. In addition, immunophenotyping revealed increase surface expressions CD44 CD69 various cell types, such CD8+ CD4 T-cells, B-cells macrophages, animals with obesity. Conclusions combination fructose supplementation promotes properly symptoms metabolic syndrome. combined nutrition can be suitable model Western diet. despite these differences, both models showed immunophenotypic that may associated cancer.
Language: Английский
Citations
3
Frontiers in Immunology, Journal Year: 2024, Volume and Issue: 15
Published: April 25, 2024
Systemic autoimmune diseases (SADs) are a significant burden on the healthcare system. Understanding complexity of peripheral immunophenotype in SADs may facilitate differential diagnosis and identification potential therapeutic targets.
Language: Английский
Citations
2
iScience, Journal Year: 2024, Volume and Issue: 27(6), P. 109882 - 109882
Published: May 4, 2024
Germline pathogenic variants in
Language: Английский
Citations
1
International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(12), P. 6311 - 6311
Published: June 7, 2024
The mammalian immune system is a Janus-faced network of well-coordinated highly specialized cells and biomolecules [...]
Language: Английский
Citations
0
APOPTOSIS, Journal Year: 2024, Volume and Issue: unknown
Published: Dec. 26, 2024
Language: Английский
Citations
0