Interactions of SARS-CoV-2 with Human Target Cells—A Metabolic View DOI Open Access
Wolfgang Eisenreich,

Julian Leberfing,

Thomas Rudel

et al.

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(18), P. 9977 - 9977

Published: Sept. 16, 2024

Viruses are obligate intracellular parasites, and they exploit the cellular pathways resources of their respective host cells to survive successfully multiply. The strategies viruses concerning how take advantage metabolic capabilities for own replication can vary considerably. most common alterations triggered by affect central carbon metabolism infected cells, in particular glycolysis, pentose phosphate pathway, tricarboxylic acid cycle. upregulation these processes is aimed increase supply nucleotides, amino acids, lipids since products crucial efficient viral proliferation. In detail, however, this manipulation may multiple sites regulatory mechanisms host-cell metabolism, depending not only on specific but also type cells. review, we report situations reprogramming different human tissues, organs that favorable acute persistent SARS-CoV-2 infection. This knowledge be fundamental development host-directed therapies.

Language: Английский

Afobazole: a potential drug candidate which can inhibit SARS CoV-2 and mimicry of the human respiratory pacemaker protein DOI
Govinda Rao Dabburu, Anjali Garg, Neelja Singhal

et al.

In Silico Pharmacology, Journal Year: 2025, Volume and Issue: 13(1)

Published: Feb. 17, 2025

Language: Английский

Citations

0
In Silico and In Vitro Studies of the Approved Antibiotic Ceftaroline Fosamil and Its Metabolites as Inhibitors of SARS-CoV-2 Replication DOI Creative Commons
Cássia P. Delgado, Pablo A. Nogara, Milene Dias Miranda

et al.

Viruses, Journal Year: 2025, Volume and Issue: 17(4), P. 491 - 491

Published: March 28, 2025

The SARS-CoV-2 proteases Mpro and PLpro are critical targets for antiviral drug development the treatment of COVID-19. 1,2,4-thiadiazole functional group is an inhibitor cysteine proteases, such as papain cathepsins. This chemical moiety also present in ceftaroline fosamil (CF), FDA-approved fifth-generation cephalosporin antibiotic. study investigates interactions between CF, its primary metabolites (M1 dephosphorylated CF M2 opened β-lactam ring) derivatives (protonated M1H M2H), open rings (open-M1H open-M2H) with evaluates CF’s effects on vitro viral replication. In silico analyses (molecular docking molecular dynamics (MD) simulations) demonstrated that potential inhibitors Mpro. Docking analysis indicated majority ligands were more stable than PLpro; however, biochemical preferred target CF. inhibited replication human Calu-3 cell model at submicromolar concentrations when added to culture medium 12 h. Our results suggest should be evaluated a repurposing agent COVID-19, considering not only but other relevant cellular pathways. Additionally, reactivity sulfur warrants further exploration protease inhibitors.

Language: Английский

Citations

0
Convergent Mechanisms in Virus-Induced Cancers: A Perspective on Classical Viruses, SARS-CoV-2, and AI-Driven Solutions DOI Creative Commons

Thorsten Rudroff

Infectious Disease Reports, Journal Year: 2025, Volume and Issue: 17(2), P. 33 - 33

Published: April 16, 2025

This perspective examines the potential oncogenic mechanisms of SARS-CoV-2 through comparative analysis with established cancer-causing viruses, integrating classical virological approaches artificial intelligence (AI)-driven analysis. The paper explores four key themes: shared between viruses and (including cell cycle dysregulation, inflammatory signaling, immune evasion, metabolic reprogramming); application AI in understanding viral oncogenesis; integration neuroimaging evidence; future research directions. author presents novel hypotheses regarding SARS-CoV-2’s mechanisms, supported by recent PET/FDG imaging studies showing persistent alterations. manuscript emphasizes transformative combining traditional methods advanced technologies for better preventing virus-induced cancers, while highlighting importance long-term monitoring COVID-19 survivors developments.

Language: Английский

Citations

0
Underestimated virus impaired cognition-more evidence and more work to do DOI Creative Commons
Maher Un Nisa Awan, Faisal Mahmood, Peng Xiao

et al.

Frontiers in Immunology, Journal Year: 2025, Volume and Issue: 16

Published: May 12, 2025

Neurodegenerative disorders (NDs) are chronic neurological diseases that can be of idiopathic, genetic, or potentially infectious origin. Although the exact cause neurodegeneration is unknown, it might result a confluence age, genetic susceptibility factors, and environmental stresses. The blood-brain barrier shields brain from majority viral infections, however neurotropic viruses able to breach this infect central nervous system. Growing research points possible connection between neurodegenerative diseases, indicating virus-induced neuroinflammation disruption neuronal protein quality control may play role in initial stages disease progression. diagnosis treatment NDs urgent challenging. Even though there limited clinical evidence support use antiviral medications their dose regimens within system (CNS), with exception acyclovir, they currently utilized treat various CNS infections. Understanding neuropathogenesis infection help targeted plans by focusing on molecular mechanisms CNS. It also helpful search for new drugs, which crucial better managing these This review focuses findings linking explores how modifications cellular functions impact development will explore therapeutic potential drugs NDs.

Language: Английский

Citations

0
Analysis of the SARS-CoV-2 inactivation mechanism using violet-blue light (405 nm) DOI Creative Commons
D Amodeo, Serena Marchi, Lia Fiaschi

et al.

Applied and Environmental Microbiology, Journal Year: 2025, Volume and Issue: unknown

Published: May 14, 2025

ABSTRACT The study evaluated the effects of violet-blue light (VBL) on cell viability and replication, carbonylation three structural proteins (S, E, N) one non-structural protein (NSP13), direct damage to RNA SARS-CoV-2. virus was exposed increasing doses VBL along with influenza A B viruses compare their susceptibility. At highest dose (21.6 J/cm 2 ), SARS-CoV-2 significantly more susceptible than viruses, a reduction in viral titer 2.33 log 10 . Viral did not show significant changes after exposure VBL, as demonstrated by next-generation sequencing real-time PCR quantification, suggesting that inactivation process does involve nucleic acid damage. To exclude role culture suspension process, experiments were performed using different dilutions Dulbecco’s modified Eagle’s medium (DMEM) phosphate-buffered saline (PBS). results indicated played secondary inactivation, increase DMEM dilution. Subsequent tests antioxidants (NAC, AsA, SOD) at concentrations prevented from 99.99% 85.43% (with SOD 0.003 mM). Carbonylation S E pronounced when suspended rather PBS, although intrinsic properties membrane crucial element consider relation its susceptibility VBL. IMPORTANCE Light-based disinfection methods are often used combination other cleaning due non-invasive nature, versatility, environmental benefits. is an effective approach it induces production reactive oxygen species reduce microbial viability. In this study, lipid peroxidation identified important factor affecting integrity function envelope, reducing ability interact host cells consequently be infectious. envelope SARS-CoV-2, composed mainly glycerophospholipids lacking cholesterol sphingolipids, appears critical susceptibility, distinguishing which have profile richer components protect against oxidative stress.

Language: Английский

Citations

0
SARS-CoV-2 uses Spike glycoprotein to control the host's anaerobic metabolism by inhibiting LDHB DOI Creative Commons
Vittoria Monaco, Ilaria Iacobucci, Luisa Canè

et al.

International Journal of Biological Macromolecules, Journal Year: 2024, Volume and Issue: 278, P. 134638 - 134638

Published: Aug. 13, 2024

Language: Английский

Citations

3
Deciphering the Longevity and Levels of SARS-CoV-2 Antibodies in Children: A Year-Long Study Highlighting Clinical Phenotypes and Age-Related Variations DOI Creative Commons
Gemma Pons-Tomàs, Rosa Pino,

Aleix Soler-García

et al.

Pathogens, Journal Year: 2024, Volume and Issue: 13(8), P. 622 - 622

Published: July 26, 2024

Background: Identifying potential factors correlated with the sustained presence of antibodies in plasma may facilitate improved retrospective diagnoses and aid appraisal pertinent vaccination strategies for various demographic groups. The main objective was to describe persistence anti-spike IgG one year after diagnosis children analyse its levels relation epidemiological clinical variables. Methods: A prospective, longitudinal, observational study conducted a university reference hospital Metropolitan Region Barcelona (Spain) (March 2020–May 2021). This included patients under 18 years age SARS-CoV-2 infection (positive PCR or antigen tests SARS-CoV-2). Clinical serological follow-up performed. Results: We 102 median 8.8 years. Anti-spike positive 98/102 (96%) 12 months infection. There were higher noted younger than 2 (p = 0.034) those pneumonia < 0.001). significant correlation observed between C-reactive protein at titre one-year 0.027). Conclusion: Anti-SARS-CoV-2 detected almost all paediatric also virus-specific antibody titres phenotype (pneumonia) (under old).

Language: Английский

Citations

0
Interactions of SARS-CoV-2 with Human Target Cells—A Metabolic View DOI Open Access
Wolfgang Eisenreich,

Julian Leberfing,

Thomas Rudel

et al.

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(18), P. 9977 - 9977

Published: Sept. 16, 2024

Viruses are obligate intracellular parasites, and they exploit the cellular pathways resources of their respective host cells to survive successfully multiply. The strategies viruses concerning how take advantage metabolic capabilities for own replication can vary considerably. most common alterations triggered by affect central carbon metabolism infected cells, in particular glycolysis, pentose phosphate pathway, tricarboxylic acid cycle. upregulation these processes is aimed increase supply nucleotides, amino acids, lipids since products crucial efficient viral proliferation. In detail, however, this manipulation may multiple sites regulatory mechanisms host-cell metabolism, depending not only on specific but also type cells. review, we report situations reprogramming different human tissues, organs that favorable acute persistent SARS-CoV-2 infection. This knowledge be fundamental development host-directed therapies.

Language: Английский

Citations

0