Integrative Transcriptomic Profiling of NK Cells and Monocytes: Advancing Diagnostic and Therapeutic Strategies for COVID-19. DOI Creative Commons

Salma LOUKMAN,

Reda Ben Mrid,

Najat Bouchmaa

et al.

Published: Oct. 21, 2024

Abstract In this study, we use integrated transcriptomic datasets from the GEO repository with purpose of investigating immune dysregulation in COVID-19. Thus, context, decided to be focused on NK cells and CD14+ monocytes gene expression, considering GSE165461 GSE198256, respectively. Other PBMCs, lung, olfactory, sensory epithelium lymph were used provide robust validation for our results. This approach gave an view responses COVID-19, pointing out a set potential biomarkers therapeutic targets special regard standards physiological conditions. IFI27, MKI67, CENPF, MBP, HBA2, TMEM158, THBD, HBA1, LHFPL2, SLA, AC104564.3 identified as key genes analysis that have critical biological processes related inflammation, regulation, oxidative stress, metabolic processes. Consequently, such are important understanding heterogeneous clinical manifestations COVID-19—from acute long-term effects now known ‘long COVID’. Subsequent additional consolidated these role diagnosis COVID-19 prediction its severity. Moreover, their enrichment pathophysiological pathways presented them intervention.The results insight into molecular dynamics caused by other monocytes. study constitutes solid basis targeted diagnostic development makes relevant contributions ongoing research efforts toward better management mitigation pandemic.

Language: Английский

Exploring the Pathophysiology of Long COVID: The Central Role of Low-Grade Inflammation and Multisystem Involvement DOI Open Access
Evgeni Gusev, Alexey Sarapultsev

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(12), P. 6389 - 6389

Published: June 9, 2024

Long COVID (LC), also referred to as Post COVID-19 Condition, Post-Acute Sequelae of SARS-CoV-2 Infection (PASC), and other terms, represents a complex multisystem disease persisting after the acute phase COVID-19. Characterized by myriad symptoms across different organ systems, LC presents significant diagnostic management challenges. Central disorder is role low-grade inflammation, non-classical inflammatory response that contributes chronicity diversity observed. This review explores pathophysiological underpinnings LC, emphasizing importance inflammation core component. By delineating pathogenetic relationships clinical manifestations this article highlights necessity for an integrated approach employs both personalized medicine standardized protocols aimed at mitigating long-term consequences. The insights gained not only enhance our understanding but inform development therapeutic strategies could be applicable chronic conditions with similar features.

Language: Английский

Citations

8
Immune activation and immune-associated neurotoxicity in Long-COVID: A systematic review and meta-analysis of 103 studies comprising 58 cytokines/chemokines/growth factors DOI
Abbas F. Almulla,

Yanin Thipakorn,

Bo Zhou

et al.

Brain Behavior and Immunity, Journal Year: 2024, Volume and Issue: 122, P. 75 - 94

Published: Aug. 9, 2024

Language: Английский

Citations

7
Long-COVID-19 autonomic dysfunction: An integrated view in the framework of inflammaging DOI

Sergio Giunta,

Chiara Giordani, María De Luca

et al.

Mechanisms of Ageing and Development, Journal Year: 2024, Volume and Issue: 218, P. 111915 - 111915

Published: Feb. 13, 2024

Language: Английский

Citations

6
Blood Biomarkers of Long COVID: A Systematic Review DOI
Callum Thomas, Mark A. Faghy, Corinna Chidley

et al.

Molecular Diagnosis & Therapy, Journal Year: 2024, Volume and Issue: 28(5), P. 537 - 574

Published: Aug. 5, 2024

Language: Английский

Citations

6
Mass cytometry revealed the circulating immune cell landscape across different Suzuki stages of Moyamoya disease DOI Creative Commons
Chenglong Liu, Peicong Ge, Bojian Zhang

et al.

Immunologic Research, Journal Year: 2024, Volume and Issue: 72(4), P. 654 - 664

Published: Feb. 20, 2024

Abstract Moyamoya disease (MMD) is a cerebrovascular disorder marked by progressive arterial narrowing, categorized into six stages known as Suzuki based on angiographic features. Growing evidence indicates pivotal role of systemic immune and inflammatory responses in the initiation advancement MMD. This study employs high-dimensional mass cytometry to reveal immunophenotypic characteristics peripheral blood cells (PBMCs) at various stages, offering insights progression PBMC samples from eight patients with early-stage MMD (Suzuki II III) later-stage IV, V, VI) were analyzed using evaluate frequency phenotype cell subtypes. We identified 15 clusters found that immunological features are composed cluster variations. In this study, we confirmed that, compared MMD, group exhibits an increase non-classical monocytes. As stage level increases, proportions plasmacytoid DCs monocyte-derived decrease. Furthermore, T cells, monocytes, DCs, PMN-MDSCs show activation canonical NF-κB signaling pathway. summarized similarities differences between patients. There potential circulating dysfunction onset development

Language: Английский

Citations

4
Long COVID as a Disease of Accelerated Biological Aging: An Opportunity to Translate Geroscience Interventions DOI
Areez Shafqat, Mary Clare Masters, Utkarsh Tripathi

et al.

Ageing Research Reviews, Journal Year: 2024, Volume and Issue: 99, P. 102400 - 102400

Published: June 28, 2024

Language: Английский

Citations

4
Indications for an antidepressive effect of thymosin alpha-1 in a small open-label proof of concept study in Common Variable Immune Deficiency patients with depression. DOI Creative Commons
D Mersha,

Sarah E. Fromme,

Frank van Boven

et al.

Brain Behavior & Immunity - Health, Journal Year: 2025, Volume and Issue: 43, P. 100934 - 100934

Published: Jan. 5, 2025

A considerable proportion (21%) of patients with common variable immunodeficiency (CVID) suffers from depression. These subjects are characterized by reduced naïve T cells and a premature cell senescence similar to that major depressive disorder (MDD). It is known essential for limbic system development/function. Treatment thymosin α1 (Tα1) capable increase the thymus output cells. To treat CVID comorbid episode Tα1 thereby improve mood. small open-label, proof concept trial. Five depressed (Hamilton Depression Rating Scale, HDRS >12) could be treated (8 weeks, 1.6 mg daily subcutaneously, 1st week, thereafter twice weekly). At start, at 8 weeks after last injection, was recorded blood samples drawn measuring memory cells, Th17 Treg hsCRP, IL-6 IL-7. Outcomes were compared those contrast group (42 MDD patients, same severity but as usual (TAU)). In all 5 decreased during treatment (with average 52%, TAU scores 36% in patients). All showed an naïve/memory CD4+ CD8+ ratios, 4 detectable levels reductions recorded. did not show such immune improvements. 8-week wash-out, depression recurred 2 most severe while continued others. Immune effects sustained wash-out. This preliminary study suggests hormone have antidepressive related correcting effects. Data urge larger placebo-controlled trials.

Language: Английский

Citations

0
Solid Organ Transplants Caused by COVID-19 Infection and the Outcome of Transplantation Post-COVID-19: A Systematic Review DOI Creative Commons

Shadi Mahmoud,

Aparajita Sarkar, Latifa AlMahmoud

et al.

Biomedicines, Journal Year: 2025, Volume and Issue: 13(2), P. 428 - 428

Published: Feb. 10, 2025

The Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) pandemic has imposed several medical and economic challenges since its onset in 2019. This is due to ability target the respiratory system as well other organs, resulting significant impacts necessitating organ transplants. Our goal compile information from existing literature investigate how COVID-19 affects outcomes following transplantation. A comprehensive search was conducted studies reporting post-COVID-19 complications. We included 45 data related solid transplants, where either recipient, organ, or donor affected by SARS-CoV-2. majority of concluded that transplantation infection could be performed safely with similar non-COVID-19 patients, regardless whether donor, recipient COVID-19. No deviation standard immunosuppression regimens surgical protocols necessary either, further re-assuring feasibility these transplants viable treatment options. applies involving lungs, kidneys, liver, heart. However, there a limited number some areas, which warrants need for additional research order reach more concrete conclusions pertaining COVID-19’s effect on

Language: Английский

Citations

0
Immune dysregulation in long covid may contribute to the development of neurological disorders through the effects of cytokines DOI Open Access
В. В. Рассохин, N. A. Аrsentieva, Zoia R. Korobova

et al.

HIV Infection and Immunosuppressive Disorders, Journal Year: 2025, Volume and Issue: 16(4), P. 7 - 16

Published: Feb. 12, 2025

Aim of the study: to investigate role proinflammatory markers and some immune cells in neural cognitive disorders long COVID patients. Materials methods. The study included 81 patients diagnosed with COVID, presence which was determined by persistence a complex multidirectional symptoms for more than 12 weeks that developed during or after COVID-19. Flow cytometry used assess lymphocyte subsets. main subpopulations were analyzed: T cells, cytotoxic B NK NKT blood plasma via multiplex xMAP analysis. Also concentrations GM-CSF, IL-1, IL-2, IL-4, IL-5, IL-6, IL-8, IL-12, IL-13, IL-17, MCP-1, MIP-1b, TNF measured. content cytokines system characterized respondents COVID. A comparative analysis data conducted individuals without history COVID-19, as well between different numbers SARS-CoV-2 cases. Results discussion. identified characteristic features affect both nervous systems. Analysis revealed absolute relative lymphopenia due decrease cells. We also noticed an increased number who suffered multiple when compared those only infected once. Cytokine increase IL-17 CCL2/MCP1 Conclusion. provides additional support theory dysregulation neurological

Language: Английский

Citations

0
Effect of obesity on the acute response to SARS-CoV-2 infection and development of post-acute sequelae of COVID-19 (PASC) in nonhuman primates DOI Creative Commons
Kristin A. Sauter,

Gabriela M Webb,

Lindsay Bader

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 19, 2025

Abstract Long-term adverse consequences of SARS-CoV-2 infection, termed “long COVID” or post-acute sequelae COVID (PASC), are a major component overall COVID-19 disease burden. Prior obesity and metabolic increase the severity acute disease, but infection also contributes to development new-onset disease. Since pandemic occurred in context global epidemic, an important question is extent which pre-existing modifies long-term responses infection. We utilized nonhuman primate model compare effects with delta variant lean obese/insulin-resistant adult male rhesus macaques over 6-month time course. While some longitudinal including viral dynamics, SARS-CoV-2-specific IgG induction, cytokine profiles, tissue persistence RNA, did not appreciably differ between obese animals, other responses, neutralizing Ab lung pathology, body weight, degree insulin sensitivity, adipocytokine temperature, nighttime activity levels were significantly different versus animals. Furthermore, several parameters animals altered following resemble those Notably, persistent changes multiple present most suggesting that PASC may be more prevalent than estimated from self-reported symptoms human studies.

Language: Английский

Citations

0