Frontiers in Immunology, Journal Year: 2024, Volume and Issue: 15
Published: Nov. 25, 2024
Hepatocellular carcinoma (HCC) is a common malignancy worldwide, and its development closely related to abnormalities in iron metabolism. This study aims systematically analyze changes metabolism the tumor microenvironment of HCC using single-cell sequencing technology, investigate potential mechanisms by which regulation affects survival liver cancer patients.
Language: Английский
Frontiers in Immunology, Journal Year: 2024, Volume and Issue: 15
Published: April 12, 2024
Surgery, chemotherapy, and endocrine therapy have improved the overall survival postoperative recurrence rates of Luminal A, B, HER2-positive breast cancers but treatment modalities for triple-negative cancer (TNBC) with poor prognosis remain limited. The effective application rapidly developing chimeric antigen receptor (CAR)-T cell in hematological tumors provides new ideas cancer. Choosing suitable specific targets is crucial applying CAR-T treatment. In this paper, we summarize therapy’s potential different subtypes based on existing research progress, especially TNBC. CAR-based immunotherapy has resulted advancements CAR-macrophages, CAR-NK cells, CAR-mesenchymal stem cells (MSCs) may be more safer treating solid tumors, such as However, tumor microenvironment (TME) side effects pose challenges to immunotherapy. cells-derived exosomes are advantageous therapy. Exosomes carrying CAR immense value provide a modality good effects. review, an overview development discuss progress CAR-expressing We elaborate TNBC prospects using CAR-MSCs
Language: Английский
Citations
13
Medical Oncology, Journal Year: 2025, Volume and Issue: 42(4)
Published: March 18, 2025
Language: Английский
Citations
1
Scientific Reports, Journal Year: 2025, Volume and Issue: 15(1)
Published: Feb. 11, 2025
Abstract Chimeric antigen receptor T (CAR-T) therapy holds promise for cancer treatment but faces challenges with using fresh patient cells, including manufacturing failures and logistical hurdles. Cryopreserved peripheral blood mononuclear cells (PBMCs) offer a potential solution, while lentiviral processes have been reported generating CAR-T from these few studies demonstrated successful PiggyBac electroporation methods. Therefore, the objectives of our study were twofold: Firstly, to conduct comparative on cryopreserved PBMCs, their respective preparations CAR-T. Secondly, establish preparation process PBMCs through optimization. The results revealed that long-term frozen viability in relatively stable manner. generated exhibited comparable expansion potential, cell phenotype, differentiation profiles, exhaustion markers, cytotoxicity against human ovarian line (SKOV-3) those derived PBMCs. Moreover, optimization, we further enhanced proliferation toxicity This approach has revolutionize production model by utilizing healthy donor instead cells. shift could mitigate issues affecting efficacy, such as suboptimal condition following illness or delays preparation.
Language: Английский
Citations
0
Journal of Orthopaedic Translation, Journal Year: 2025, Volume and Issue: 52, P. 70 - 84
Published: April 10, 2025
Language: Английский
Citations
0
Frontiers in Pharmacology, Journal Year: 2025, Volume and Issue: 16
Published: April 11, 2025
Background Ubiquitination, a critical post-translational modification, plays pivotal role in regulating protein stability and activity, influencing various aspects of cancer development, including metabolic reprogramming, immune evasion, tumor progression. However, the specific ubiquitination hepatocellular carcinoma (HCC), particularly relation to microenvironment (TME), remains poorly understood. This study aims systematically explore shaping TME HCC, with focus on its impact progression modulation. Methods We performed bioinformatics analysis by integrating multiple publicly available HCC datasets assess status across cell types TME, plasma cells, fibroblasts, endothelial epithelial-mesenchymal transition (EMT) cells. Ubiquitination scores were calculated categorize these types, survival data, along spatial transcriptomics, employed evaluate how different levels influence In vitro experiments, such as transwell, CCK8, wound healing assays, used further investigate key gene UBE2C phenotypes. Results Our revealed that ubiquitination-related genes are significantly upregulated tissues, high expression correlating poor prognosis patients. Pathway showed enriched processes cycle regulation, DNA repair, p53 signaling. These pathways contribute promoting proliferation, facilitating matrix remodeling, enhancing angiogenesis. Notably, UBE2C, enzyme, appears play potentially inhibiting anti-tumor responses reducing system’s ability recognize eliminate Furthermore, experimental data confirmed overexpression promotes invasion, metastasis, supporting remodeling. Conclusion reveals multifaceted regulatory roles HCC. not only supports proliferation anti-apoptotic functions within cells but also modulating activity stromal Among all genes, emerges potential prognostic biomarker therapeutic target offering new directions for precision treatment future.
Language: Английский
Citations
0
Frontiers in Oncology, Journal Year: 2025, Volume and Issue: 14
Published: Jan. 21, 2025
Hepatocellular carcinoma (HCC) is one of the most common lethal cancers worldwide. Natural killer cells (NK cells) play a key role in liver immunosurveillance, but tumor microenvironment, NK are readily depleted, as evidenced by down-regulation activating receptors, reduced cytokine secretion, and attenuated killing function. The up-regulation inhibitory such PD-1, TIM-3, LAG-3, further exacerbates depletion cells. Combined blockade strategies targeting these immunosuppressive mechanisms, combination PD-1 inhibitors with other pathways (eg. TIM-3 LAG-3), have shown potential to reverse cell exhaustion preclinical studies. This article explores promise innovative HCC immunotherapy, providing new therapeutic directions for optimizing function improving drug sensitivity.
Language: Английский
Citations
0
Molecular Immunology, Journal Year: 2025, Volume and Issue: 182, P. 54 - 61
Published: April 4, 2025
Natural Killer (NK) cells are a vital element of the innate immune system, and NK cell-based therapies have demonstrated efficacy against various malignancies. However, targeting solid tumors has been challenging due to low infiltration into effective evasion strategies employed by tumors. Recent studies shown that cell derived nanovesicles (NK-NV) can not only replicate functions but also offer more advantages in clinical applications. They capable transporting cellular components such as proteins, nucleic acids, lipids across distances, thereby facilitating intercellular communication among within tumor microenvironment (TME). With progress nanomedical technology, these vesicles be engineered carry range functional elements therapeutic agents enhance their antitumoral capabilities. In this review, we summarize current available literature on NK-NVs, discuss potential biological role non-coding RNAs (ncRNAs), explore application treatment
Language: Английский
Citations
0
International Journal of Pharmaceutics, Journal Year: 2025, Volume and Issue: 675, P. 125573 - 125573
Published: April 1, 2025
Language: Английский
Citations
0
Phytomedicine, Journal Year: 2024, Volume and Issue: 133, P. 155916 - 155916
Published: July 27, 2024
Language: Английский
Citations
2
Frontiers in Immunology, Journal Year: 2024, Volume and Issue: 15
Published: Oct. 28, 2024
Current research is focused on utilizing EVs as a biopsy tool to improve the diagnostic accuracy of HCC, reduce surgical risk, and explore their potential in modulating drug resistance advancing immunotherapeutic strategies. Extracellular vesicles (EVs) have been increasingly recognized important non-invasive biomarkers hepatocellular carcinoma (HCC) due presence variety biomolecules within them, such proteins RNAs, etc. play key role early detection, diagnosis, treatment, prognostic monitoring HCC. These influence development HCC therapeutic response ways, including influencing tumor microenvironment, resistance, participating immune regulatory mechanisms. In addition, specific molecules miRNAs are regarded markers for treatment recurrence which certain space prospects. this paper, we summarize aspects markers, also discuss questions that may be faced markers.
Language: Английский
Citations
0