Correlation of CCL3 with Ferroptosis in Intervertebral Disc Degeneration: An Insight into Prognostic Significance DOI Creative Commons
He Chen, Peng Xu,

Leijing Mao

et al.

Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 11, 2024

Abstract Background Intervertebral disc degeneration (IVDD) is a prevalent and debilitating condition associated with low back pain (LBP). Despite its significant impact, effective diagnostic markers for early-stage IVDD remain elusive. Recent research has implicated ferroptosis, newly recognized form of programmed cell death, in the pathogenesis IVDD, particularly involving disturbances iron homeostasis. Additionally, CC Chemokine Ligand 3 (CCL3) been linked to macrophage migration progression yet precise prognostic utility remains uncertain. Objective This study aims elucidate underlying mechanisms ferroptosis involvement CCL3 objective establishing their significance. By uncovering these mechanisms, novel biomarkers therapeutic targets diagnosis prognosis may be identified. Methods Single-cell sequencing data were acquired from TCGA database, range bioinformatics methods employed comprehensive analysis. Furthermore, validation experiments conducted using vitro techniques, including analysis human tissue samples, co-culture assays neutralizing antibodies, quantitative real-time polymerase chain reaction (qRT-PCR), Western blotting. Results Our findings suggest that holds promise as biomarker IVDD. Validation demonstrated functions via pAMPK/AMPK pathway, thereby modulating apoptosis impacting Conclusion underscores potential patients Further investigations are warranted explore strategies targeting CCL3, ultimately enhancing management

Language: Английский

CRYAB is upregulated and predicts clinical prognosis in kidney renal clear cell carcinoma DOI
Hao Ren, Xinyu Chen,

Meiling Ji

et al.

IUBMB Life, Journal Year: 2025, Volume and Issue: 77(1)

Published: Jan. 1, 2025

Abstract Clear cell renal carcinoma (KIRC) is the most prevalent subtype of (RCC), accounting for 70% to 80% all RCC cases. The CRYAB (αB‐crystallin) gene broadly expressed across various human tissues, yet its role in KIRC progression remains unclear. This study aims elucidate function and assess potential as a biomarker early diagnosis, therapeutic targeting, prognosis. In our report, we found that was dramatically upregulated KIRC, expression associated with TNM stage, pathological age. Also, patients higher exhibited poor survival overexpression led enhanced tumor proliferation. Vice versa, downregulation resulted decreased proliferation vitro. Mechanistically, Gene set enrichment analysis plots showed survival. Consistently, these effects were increased AKT signaling BCL‐2 expression. Furthermore, also observed levels negatively correlated immunocyte infiltration. conclusion, findings suggested could be regarded latent

Language: Английский

Citations

0
Single-cell RNA sequencing revealed PPARG promoted osteosarcoma progression: based on osteoclast proliferation DOI Creative Commons
Lei Sun, Jinhao Zhang,

Zhikai Xiahou

et al.

Frontiers in Immunology, Journal Year: 2025, Volume and Issue: 15

Published: Jan. 28, 2025

Background Osteosarcoma (OS) is one of the most common primary malignant bone tumors, primarily originating from mesenchymal tissue. It notorious for its high invasiveness, disability rate, mortality and poor prognosis. In metastatic destruction can promote cancer progression, which closely related to osteoclast activation imbalance between osteoblasts osteoclasts. A large number studies confirmed that osteoclasts are an important part OS, play active role in destroying homeostasis promoting progress OS. Therefore, we conducted a detailed study at single cell level, aiming find new OS therapeutic targets prevent tumor progression local spread. Methods We analyzed single-cell sequencing data patients usedMonocle2, Cytotrace, Slingshot software analyze pseudo-sequential trajectory during progression. CellChat was used reveal communication cells. PySCENIC identify transcription factors Finally, further demonstrated results by RT-qPCR analysis, CCK-8 assay, wound healing Transwell etc. Results Through analysis identified highly specific subgroup, C2MKI67+ Osteoclast. The key signaling pathway APP top 1 factor PPARG this subgroup played essential roles proliferation differentiation. Given pivotal speculated these pathways could emerge as novel targets, offering innovative strategies treatment. Conclusion This enhanced our understanding through scRNA-seq. Furthermore, discovered amplifies proliferation, resulting excessive resorption degradation matrix, thereby creating favorable environment growth. By innovatively targeting PPARG, it affected thus progression; work offered insights directions clinical treatment patients.

Language: Английский

Citations

0
FMNL2/SRC-mediated androgen receptor translocation into the nucleus promotes enzalutamide resistance of prostate cancer. DOI Creative Commons
Jianpeng Yu, Yukui Gao, Mingpeng Zhang

et al.

iScience, Journal Year: 2025, Volume and Issue: unknown, P. 112205 - 112205

Published: March 1, 2025

Enzalutamide, a second-generation androgen receptor (AR) antagonist, has represented the association with improved overall survival in men prostate cancer (PCa). However, PCa patients receiving enzalutamide will eventually develop resistance through various mechanisms without effective regimens. Here, we observed higher level of formin-like 2 (FMNL2) enzalutamide-resistant cells. Functionally, FMNL2 knockdown partially re-sensitized Mechanistically, directly interacted SRC kinase FMNL2-FH1 and SRC-SH3 domain, which induced AR translocation from cytoplasm to nucleus, resulting increased expression AR-targeted genes leading enzalutamide. Consistently, inhibitor dasatinib rescued sensitivity inhibited proliferation Taken together, our findings demonstrate substantial role for FMNL2/SRC interaction regulation translocation, suggesting that targeting FMNL2-mediated activation might be potential therapeutic strategy could an option.

Language: Английский

Citations

0
CCL3 correlates with ferroptosis in intervertebral disc degeneration and its prognostic significance DOI Creative Commons
He Chen, Peng Xu,

Leijing Mao

et al.

Scientific Reports, Journal Year: 2025, Volume and Issue: 15(1)

Published: April 9, 2025

Language: Английский

Citations

0
Comprehensive gene set enrichment and variation analyses identify SUV39H1 as a potential prognostic biomarker for glioblastoma immunorelevance DOI Creative Commons
Jixuan Liu,

Qian Luo,

Haoran Zhao

et al.

Computational and Structural Biotechnology Journal, Journal Year: 2024, Volume and Issue: 23, P. 4161 - 4176

Published: Nov. 6, 2024

Glioblastoma (GBM) is the most common intracranial malignancy.

Language: Английский

Citations

0
Proteomic Profiling of Pre- and Post-Surgery Saliva of Glioblastoma Patients: A Pilot Investigation DOI Open Access
Alexandra Muntiu, Fabiana Moresi, Federica Vincenzoni

et al.

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(23), P. 12984 - 12984

Published: Dec. 3, 2024

Glioblastoma multiforme (GBM) is an extremely aggressive brain tumor characterized by a high infiltration capability and recurrence rate. Early diagnosis crucial to improve the prognosis personalize therapeutic approach. This research explored, LC-MS proteomic analysis after proteolytic digestion, molecular profile of pre- post-operative saliva pools from newly diagnosed (ND) GBM patients comparing different times collection (R). CYCS, PRDX2, RAB1C, PSMB1, KLK6, TMOD3, PAI2, PLBD1, CAST, AHNAK, all involved in processes invasiveness chemo- radio-resistance, were found depict pre-surgery both ND R GBM. PADI4 CRYAB proteins, identified among most abundant proteins exclusive classified as elevated glioma, could have potential role disease biomarkers. Selected panels S100 potentially differentiate patient saliva. TPD52 IGKV3, exclusively saliva, be additionally distinctive relapse. Among pools, label-free relative quantitation showed statistically significant levels TXN, SERPINB5, FABP5, S100A11 between pools. All these higher ND_ R_T0 with respect CTRL modulation surgery or chemo-radiotherapy combined treatment, suggesting panel predictive prognostic These results highlight confirm that biofluid featured for easily accessible low collection, promising source biomarkers, showing new opportunities development targeted therapies diagnostic tools.

Language: Английский

Citations

0
Correlation of CCL3 with Ferroptosis in Intervertebral Disc Degeneration: An Insight into Prognostic Significance DOI Creative Commons
He Chen, Peng Xu,

Leijing Mao

et al.

Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 11, 2024

Abstract Background Intervertebral disc degeneration (IVDD) is a prevalent and debilitating condition associated with low back pain (LBP). Despite its significant impact, effective diagnostic markers for early-stage IVDD remain elusive. Recent research has implicated ferroptosis, newly recognized form of programmed cell death, in the pathogenesis IVDD, particularly involving disturbances iron homeostasis. Additionally, CC Chemokine Ligand 3 (CCL3) been linked to macrophage migration progression yet precise prognostic utility remains uncertain. Objective This study aims elucidate underlying mechanisms ferroptosis involvement CCL3 objective establishing their significance. By uncovering these mechanisms, novel biomarkers therapeutic targets diagnosis prognosis may be identified. Methods Single-cell sequencing data were acquired from TCGA database, range bioinformatics methods employed comprehensive analysis. Furthermore, validation experiments conducted using vitro techniques, including analysis human tissue samples, co-culture assays neutralizing antibodies, quantitative real-time polymerase chain reaction (qRT-PCR), Western blotting. Results Our findings suggest that holds promise as biomarker IVDD. Validation demonstrated functions via pAMPK/AMPK pathway, thereby modulating apoptosis impacting Conclusion underscores potential patients Further investigations are warranted explore strategies targeting CCL3, ultimately enhancing management

Language: Английский

Citations

0