Small Interfering RNA in Kidney Diseases: Promises and Limitations DOI Creative Commons
Attilio Losito,

Giuseppe Solano

Kidney and Dialysis, Journal Year: 2024, Volume and Issue: 5(1), P. 1 - 1

Published: Dec. 29, 2024

Small interfering RNAs (siRNAs) are short, double-stranded RNA molecules that play a crucial role in the regulation of gene expression, particularly through natural process called interference (RNAi). Their discovery, about 25 years ago, paved way for whole series research leading to synthetic molecules. The silencing potential these siRNAs was initially oriented towards diseases resulting from genetic dysfunctions. This led development first approved human use hereditary transthyretin amyloidosis. Subsequently, field application expanded beyond confines diseases. refinement pharmacological techniques has synthesis variety capable blocking production individual proteins responsible various disease conditions, thus expanding their therapeutic application. kidney also been affected by this new tool, largely indirectly but also, with some difficulty, directly. structural complexity made search targeting its components very challenging. Nevertheless, results technology beginning be seen experimental animals and humans. have treatment amyloidosis patisiran oxalosis lumasiran nedosiran. Studies ongoing as anti-complement drugs IgA nephropathy, angiotensinogen inhibitors hypertension, or against mediators acute injury. In review, biological mechanisms underlying briefly exposed. analyzed discussed.

Language: Английский

Autoantibodies and therapeutic antibodies against complement Factor H DOI Creative Commons

Sandrine Nugteren,

Haiyu Wang, Cees van Kooten

et al.

Immunology Letters, Journal Year: 2025, Volume and Issue: unknown, P. 107002 - 107002

Published: March 1, 2025

Language: Английский

Citations

1
Development of point-of-care tests for urinary bladder cancer – an historic review and view to future prospectives DOI
Thorsten Ecke, Johan Styrke, Kiran Jagarlamudi

et al.

Urologic Oncology Seminars and Original Investigations, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 1, 2025

Language: Английский

Citations

0
Effective long-term treatment with moss-produced factor H by overcoming the antibody response in a mouse model of C3G DOI Creative Commons
Todor Tschongov,

Swagata Konwar,

Jessika Kleindienst

et al.

Frontiers in Immunology, Journal Year: 2025, Volume and Issue: 16

Published: March 7, 2025

Complement-associated disorders are caused by the dysregulation and disbalance of complement system, especially excessive activation. Most drugs that target system designed to inhibit pathway at either proximal or terminal levels. The use a natural regulator such as factor H (FH) could provide superior treatment option restoring balance an overactive system. We recently reported moss-based production analog human FH with optimized glycan profile (CPV-104), which showed in vitro vivo characteristics comparable its counterpart. Here, we follow up our previous work, focusing more detail on time course long-term efficacy CPV-104 FH-deficient ( –/– ) mice. analysis effects following multiple injections into mice was previously hindered immune response, so developed protocol for sustained depletion CD20 + B-cells CD4 T-cells, preventing antibody formation without influencing C3G phenotype. Using this dual-depletion method, were able complete dosing interval experiments mice, administering three different intervals. Repeated administration lastingly resolve C3 deposits, offering additional rationale clinical testing patients. Moreover, novel method has potential adaptation mouse models, allowing doses other therapeutic proteins.

Language: Английский

Citations

0
Chronic kidney disease enhances alternative pathway activity: a new paradigm DOI Creative Commons
Diana Jalal, Joshua M. Thurman, Richard J. Smith

et al.

Journal of Clinical Investigation, Journal Year: 2025, Volume and Issue: 135(9)

Published: May 1, 2025

Reduced kidney function is associated with increased risk of cardiovascular disease in addition to progression. Kidney considered an inflammatory state, based on elevated levels C-reactive protein and cytokines. A key mediator progression the setting reduced systemic vascular inflammation. However, exact pathways that link chronic (CKD) inflammation remain incompletely understood. For decades it has been known factor D, main activator alternative complement pathway, plasma patients function. Recent biomarker evidence suggests pathway activation this setting. CKD, therefore, seems alter balance proteins, promoting potentially exacerbating complement-mediated diseases CKD-associated complications. In manuscript, we review impact biomarkers implications Importantly, highlight need for ongoing research efforts may lead opportunities target withx goal improving outcomes persons

Language: Английский

Citations

0
Enhancing complement activation by therapeutic anti-tumor antibodies: Mechanisms, strategies, and engineering approaches DOI Creative Commons
Vitalijs Ovcinnikovs, Karin Dijkman, Gijs G. Zom

et al.

Seminars in Immunology, Journal Year: 2024, Volume and Issue: 77, P. 101922 - 101922

Published: Dec. 31, 2024

Language: Английский

Citations

2
From genomic insights to clinical hope: Targeting NEU1 in IgA nephropathy DOI
Cong Zhao, Mingzhu Zhang,

Leying Zhao

et al.

International Immunopharmacology, Journal Year: 2024, Volume and Issue: 132, P. 112051 - 112051

Published: April 9, 2024

Language: Английский

Citations

0
Oxidative stress-related genes as diagnostic markers for endometriosis and their associated immunoassays DOI Creative Commons

Yanlun Song,

Hui Wu, Jian Wang

et al.

Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown

Published: Nov. 21, 2024

Abstract Purpose Endometriosis (EMT) affects millions of women worldwide and is closely associated with the body's response to oxidative stress. The role stress markers in diagnosis treatment endometriosis a potentially fruitful avenue research. Methods In this study, we employed machine learning approach model screen key biomarkers, integrating five independent datasets from Omnibus (GEO) database construct comprehensive training set. identification genes was achieved through process cross-referencing aim locating those that were differentially expressed known be involved Nine algorithms for selection, followed by evaluation immune infiltration correlation single sample gene set enrichment analysis (ssGSEA) CIBERSORT algorithm. Results After comparing performance different algorithms, gradient boosting algorithm (GBM) selected as best model. Eventually it screened featured (FOS, CFH, AOX1, FMO1, FCGR2B). expression patterns these showed diagnostic predictive potential constructed nomograms external validation. addition, association pregnancy status disease severity explored. results significant between system. Conclusions This study identifies at intersection stress, thereby providing reliable molecular clinical application. offers new subsequent endometriosis.

Language: Английский

Citations

0
The Neisseria meningitidis Urethritis Clade (NmUC) Acts as a “Chimeric Pathogen” During Infection of Primary, Human Male, Urethral Epithelial Cells DOI
Yih‐Ling Tzeng, Danillo Lucas Alves Espósito,

Andrew G Nederveld

et al.

The Journal of Infectious Diseases, Journal Year: 2024, Volume and Issue: unknown

Published: Dec. 5, 2024

Clusters of male urethritis cases, caused by a novel clade non-groupable Neisseria meningitidis (NmUC, "the clade"), have been reported globally. Genetic features unique to NmUC isolates include: the acquisition gonococcal denitrification loci, norB-aniA; factor H binding protein (fHbp) variant; and loss group C capsule intrinsic lipooligosaccharide sialylation. We hypothesized that these characteristics might confer colonization survival advantage during urethral infection relative non-clade meningitidis.

Language: Английский

Citations

0
Small Interfering RNA in Kidney Diseases: Promises and Limitations DOI Creative Commons
Attilio Losito,

Giuseppe Solano

Kidney and Dialysis, Journal Year: 2024, Volume and Issue: 5(1), P. 1 - 1

Published: Dec. 29, 2024

Small interfering RNAs (siRNAs) are short, double-stranded RNA molecules that play a crucial role in the regulation of gene expression, particularly through natural process called interference (RNAi). Their discovery, about 25 years ago, paved way for whole series research leading to synthetic molecules. The silencing potential these siRNAs was initially oriented towards diseases resulting from genetic dysfunctions. This led development first approved human use hereditary transthyretin amyloidosis. Subsequently, field application expanded beyond confines diseases. refinement pharmacological techniques has synthesis variety capable blocking production individual proteins responsible various disease conditions, thus expanding their therapeutic application. kidney also been affected by this new tool, largely indirectly but also, with some difficulty, directly. structural complexity made search targeting its components very challenging. Nevertheless, results technology beginning be seen experimental animals and humans. have treatment amyloidosis patisiran oxalosis lumasiran nedosiran. Studies ongoing as anti-complement drugs IgA nephropathy, angiotensinogen inhibitors hypertension, or against mediators acute injury. In review, biological mechanisms underlying briefly exposed. analyzed discussed.

Language: Английский

Citations

0