CRYAB is upregulated and predicts clinical prognosis in kidney renal clear cell carcinoma

IUBMB Life, Journal Year: 2025, Volume and Issue: 77(1)

Published: Jan. 1, 2025

Abstract Clear cell renal carcinoma (KIRC) is the most prevalent subtype of (RCC), accounting for 70% to 80% all RCC cases. The CRYAB (αB‐crystallin) gene broadly expressed across various human tissues, yet its role in KIRC progression remains unclear. This study aims elucidate function and assess potential as a biomarker early diagnosis, therapeutic targeting, prognosis. In our report, we found that was dramatically upregulated KIRC, expression associated with TNM stage, pathological age. Also, patients higher exhibited poor survival overexpression led enhanced tumor proliferation. Vice versa, downregulation resulted decreased proliferation vitro. Mechanistically, Gene set enrichment analysis plots showed survival. Consistently, these effects were increased AKT signaling BCL‐2 expression. Furthermore, also observed levels negatively correlated immunocyte infiltration. conclusion, findings suggested could be regarded latent

Language: Английский

Construction and Validation of a T Cell Exhaustion–Related Prognostic Signature in Cholangiocarcinoma

International Journal of Genomics, Journal Year: 2025, Volume and Issue: 2025(1)

Published: Jan. 1, 2025

Objective: T cell exhaustion (TEX) is a critical determinant of immune resistance. This study was performed to investigate the key genes linked TEX in cholangiocarcinoma (CCA) and construct TEX-associated gene signature forecast prognosis patients with CCA. Methods: Based on expression data acquired from E-MTAB-6389 dataset, TEX-related modules module were identified using weighted coexpression network analysis (WGCNA). Subsequently, prognostic built by univariate least absolute shrinkage selection operator (LASSO) Cox regression analysis. The infiltration each CCA sample evaluated single-sample set enrichment (ssGSEA) package, followed single-cell RNA sequencing (scRNA-seq) Furthermore, experimentally validated cells quantitative reverse transcriptase polymerase chain reaction (qRT-PCR) western blot Results: A total 15 23 identified. Then, four-gene related established, containing Palladin, Cytoskeletal Associated Protein (PALLD), Member RAS Oncogene Family (RAB31), ADAM Metallopeptidase With Thrombospondin Type 1 Motif 2 (ADAMTS2), WISP1, which could predict Moreover, neutrophils, endothelial cells, B exhibited significant samples, these four both significantly positively correlated while negatively neutrophils. 13 types annotated after scRNA-seq Notably, RAB31 mainly highly expressed monocytes, macrophages, DC2 (Dendritic Cells 2), DC3 3), PALLD, ADAMTS2, WISP1 overexpressed fibroblasts. experimental validation revealed that levels RAB31, consistent trend results bioinformatics Conclusion: developed genes, including might be powerful predictor for These

Language: Английский

Disulfidptosis in tumor progression

Cell Death Discovery, Journal Year: 2025, Volume and Issue: 11(1)

Published: April 28, 2025

Abstract Disulfidptosis, a regulated cell death modality driven by the cystine transporter solute carrier family 7 member 11 (SLC7A11), is characterized actin cytoskeleton collapse under glucose starvation. This review systematically elucidates pivotal role of disulfidptosis in tumor metabolic reprogramming, with focus on its molecular mechanisms and distinctions from other pathways. The core include SLC7A11-mediated overload NRF2/c-Myc-regulated pentose phosphate pathway activation. By integrating multiomics data single-cell transcriptomics, we comprehensively decipher heterogeneous expression patterns disulfidptosis-related genes (DRGs) their dynamic interplay immune microenvironment remodeling. Furthermore, coexpression networks DRGs long noncoding RNAs (DRLs) offer novel insights into diagnosis, prognosis, targeted therapy. Therapeutically, SLC7A11 inhibitors (e.g., HG106) BAY-876) demonstrate efficacy exploiting vulnerabilities, whereas natural compounds synergizing checkpoint blockade provide strategies to counteract immunosuppressive microenvironments. Through interdisciplinary collaboration clinical translation, research holds transformative potential redefining precision oncology.

Language: Английский