Delineation of chicken immune markers in the era of omics and multicolor flow cytometry

Frontiers in Veterinary Science, Journal Year: 2024, Volume and Issue: 11

Published: May 23, 2024

Multiparameter flow cytometry is a routine method in immunological studies incorporated biomedical, veterinary, agricultural, and wildlife research routinely used veterinary clinical laboratories. Its use the diagnostics of poultry diseases still limited, but due to continuous expansion reagents cost reductions, this may change near future. Although structure function avian immune system show commonalities with mammals, at molecular level, there often low homology across species. The cross-reactivity mammalian therefore low, nevertheless, list study chicken cells increasing. Recent improvement multicolor antibody panels for has resulted more detailed analysis by allowed discovery novel leukocyte cell subpopulations. In article, we present an overview guidance needed perform using samples common pitfalls avoid.

Language: Английский

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Characterization of a novel chicken γδ TCR-specific marker

Developmental & Comparative Immunology, Journal Year: 2024, Volume and Issue: 161, P. 105250 - 105250

Published: Aug. 17, 2024

Chickens are a species with high number of γδ T cells in various tissues. Despite their abundance, poorly characterized chickens, partially due to lack specific reagents characterize these cells. Up until now, the TCR1 clone has been only cell-specific monoclonal antibody (mAb) chickens and additional for cell subsets needed. In order address this issue, new mAb were generated our laboratory by immunizing mice vitro cultured an initial flow cytometric screen mAb, "8D2", displayed interesting staining pattern that mirrored TCR up- downregulation line D4 over time, prompting us further. We compared expression unknown 8D2 epitope combination across primary splenocytes, peripheral blood lymphocytes intestinal epithelial cells, consistently labeled subset

Language: Английский

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Entschlüsselung der T-Zell-Antwort des Haushuhns

BIOspektrum, Journal Year: 2024, Volume and Issue: 30(5), P. 527 - 529

Published: Sept. 1, 2024

Language: Английский

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