Evaluation of a novel intramuscular prime/intranasal boost vaccination strategy against influenza in the pig model DOI Creative Commons

Robin Avanthay,

Obdulio García-Nicolás, Nicolas Ruggli

et al.

PLoS Pathogens, Journal Year: 2024, Volume and Issue: 20(8), P. e1012393 - e1012393

Published: Aug. 8, 2024

Live-attenuated influenza vaccines (LAIV) offer advantages over the commonly used inactivated split vaccines. However, finding optimal balance between sufficient attenuation and immunogenicity has remained a challenge. We recently developed an alternative LAIV based on 2009 pandemic H1N1 virus with truncated NS1 protein lacking PA-X expression (NS1(1-126)-ΔPAX). This showed blunted replication elicited strong innate immune response. In present study, we evaluated efficacy of this vaccine candidate in porcine animal model as pertinent vivo system. Immunization pigs via nasal route novel NS1(1-126)-ΔPAX did not cause disease mucosal response that completely blocked homologous challenge respiratory tract. observed prolonged shedding our from upper To improve safety, prime/boost vaccination strategy combining primary intramuscular immunization haemagglutinin-encoding propagation-defective vesicular stomatitis (VSV) replicon, followed by secondary route. two-step procedure significantly reduced shedding, increased production specific serum IgG, neutralizing antibodies, Th1 memory cells, resulted sterilizing immunity against conclusion, prime/intranasal boost regimen interferes transmission, feature will help combat epidemics pandemics.

Language: Английский

SARS-CoV-2 XBB.1.5 mRNA booster vaccination elicits limited mucosal immunity DOI
Ninaad Lasrado, Marjorie Rowe, Katherine McMahan

et al.

Science Translational Medicine, Journal Year: 2024, Volume and Issue: 16(770)

Published: Oct. 23, 2024

Current COVID-19 vaccines provide robust protection against severe disease but minimal acquisition of infection. Intramuscularly administered induce serum neutralizing antibodies (NAbs), their ability to boost mucosal immune responses remains be determined. In this study, we show that the XBB.1.5 messenger RNA (mRNA) boosters result in increased neutralization multiple acute respiratory syndrome coronavirus 2 (SARS-CoV-2) variants humans, including dominant circulating variant JN.1. contrast, found mRNA booster did not augment NAbs or IgA responses, although SARS-CoV-2 XBB infection substantially antibody responses. These data demonstrate current enhance peripheral do robustly increase Our highlight a separation between and systems humans emphasize importance developing next-generation immunity protect virus infections.

Language: Английский

Citations

8
A SARS-CoV-2 mucosal nanovaccine based on assembly of maltodextrin, STING agonist and polyethyleneimine DOI
Yu Tian, Liang Hu, Qingrui Huang

et al.

International Journal of Biological Macromolecules, Journal Year: 2025, Volume and Issue: unknown, P. 139395 - 139395

Published: Jan. 1, 2025

Language: Английский

Citations

0
Evaluation of a novel intramuscular prime/intranasal boost vaccination strategy against influenza in the pig model DOI Creative Commons

Robin Avanthay,

Obdulio García-Nicolás, Nicolas Ruggli

et al.

PLoS Pathogens, Journal Year: 2024, Volume and Issue: 20(8), P. e1012393 - e1012393

Published: Aug. 8, 2024

Live-attenuated influenza vaccines (LAIV) offer advantages over the commonly used inactivated split vaccines. However, finding optimal balance between sufficient attenuation and immunogenicity has remained a challenge. We recently developed an alternative LAIV based on 2009 pandemic H1N1 virus with truncated NS1 protein lacking PA-X expression (NS1(1-126)-ΔPAX). This showed blunted replication elicited strong innate immune response. In present study, we evaluated efficacy of this vaccine candidate in porcine animal model as pertinent vivo system. Immunization pigs via nasal route novel NS1(1-126)-ΔPAX did not cause disease mucosal response that completely blocked homologous challenge respiratory tract. observed prolonged shedding our from upper To improve safety, prime/boost vaccination strategy combining primary intramuscular immunization haemagglutinin-encoding propagation-defective vesicular stomatitis (VSV) replicon, followed by secondary route. two-step procedure significantly reduced shedding, increased production specific serum IgG, neutralizing antibodies, Th1 memory cells, resulted sterilizing immunity against conclusion, prime/intranasal boost regimen interferes transmission, feature will help combat epidemics pandemics.

Language: Английский

Citations

0