Cells, Journal Year: 2024, Volume and Issue: 13(20), P. 1678 - 1678
Published: Oct. 11, 2024
This study evaluated ethosomes as a novel nanodelivery system for nutlin-3a, known MDM2 inhibitor and activator of the p53 pathway, to improve nutlin-3a's poor solubility, limiting its bio-distribution therapeutic efficacy. The potential nutlin-3a-loaded was investigated on two in vitro models melanoma: HT144 cell line
Language: Английский
Frontiers in Immunology, Journal Year: 2024, Volume and Issue: 15
Published: July 4, 2024
Melanoma, a malignant skin cancer arising from melanocytes, exhibits rapid metastasis and high mortality rate, especially in advanced stages. Current treatment modalities, including surgery, radiation, immunotherapy, offer limited success, with immunotherapy using immune checkpoint inhibitors (ICIs) being the most promising. However, rate underscores urgent need for robust, non-invasive biomarkers to predict patient response adjuvant therapies. The microenvironment of melanoma comprises various cells, which influence tumor growth response. Melanoma cells employ multiple mechanisms escape, defects recognition epithelial-mesenchymal transition (EMT), collectively impact efficacy. Single-cell analysis technologies, such as single-cell RNA sequencing (scRNA-seq), have revolutionized understanding heterogeneity dynamics. These technologies facilitate identification rare cell populations, co-expression patterns, regulatory networks, offering deep insights into progression, response, therapy resistance. In realm biomarker discovery melanoma, has demonstrated significant potential. It aids uncovering cellular composition, gene profiles, novel markers, thus advancing diagnosis, treatment, prognosis. Additionally, tumor-associated antibodies specific genetic markers identified through hold promise predictive biomarkers. Despite these advancements, challenges RNA-protein expression discrepancies persist, necessitating further research. Nonetheless, remains powerful tool elucidating underlying resistance, ultimately contributing development personalized therapies improved outcomes.
Language: Английский
Citations
3
International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(21), P. 11502 - 11502
Published: Oct. 26, 2024
Amelanotic melanoma (AM) is a subtype of hypomelanotic or completely amelanotic melanoma. AM rare that exhibits higher recurrence rate and aggressiveness as well worse surveillance than typical shows dysregulation melanin production, cell cycle control, apoptosis pathways. Knowing these pathways has an application in medicine due to targeted therapies based on the inhibiting elements abovementioned Therefore, we summarized discussed biochemical molecular induction personalized approaches, clinical management, future directions fact relatively rare. commonly misdiagnosed. Hence, role biomarkers becoming significant. Nonetheless, there shortage specific AM. BRAF, NRAS, c-KIT genes are main targets therapy. However, BRAF KIT varied among studies. inhibitors combined with MAK demonstrate better results. Immune checkpoint targeting CTLA-4 programmed death receptor 1 (PD-1) show outcomes separately. Fecal microbiota transplantation may overcome resistance immune therapy Immune-modulatory vaccines against indoleamine 2,3-dioxygenase (IDO) PD ligand (PD-L1) nivolumab be efficient treatment.
Language: Английский
Citations
3
Cancers, Journal Year: 2024, Volume and Issue: 16(16), P. 2861 - 2861
Published: Aug. 16, 2024
Cutaneous melanoma still represents a significant health burden worldwide, being responsible for the majority of skin cancer deaths. Key advances in therapeutic strategies have significantly improved patient outcomes; however, most patients experience drug resistance and tumor relapse. Cancer stem cells (CSCs) are small subpopulation different tumors, including melanoma, endowed with distinctive capacities self-renewal differentiation into bulk cells. Melanoma CSCs characterized by expression specific biomarkers intracellular pathways; moreover, they play pivotal role onset, progression resistance. In recent years, great efforts been made to dissect molecular mechanisms underlying protumor activities provide basis novel CSC-targeted therapies. Herein, we highlight intricate crosstalk between bystander microenvironment (TME), immune cells, endothelial cancer-associated fibroblasts (CAFs), its progression. Specifically, discuss peculiar escape host surveillance, recruit immunosuppressive educate toward an phenotype. We also address currently investigated that could pave way new promising approaches care.
Language: Английский
Citations
2
Frontiers in Immunology, Journal Year: 2024, Volume and Issue: 15
Published: July 30, 2024
Mucosal melanoma (MM) poses a significant clinical challenge due to its aggressive nature and limited treatment options. In recent years, immunotherapy has emerged as promising strategy for MM, with particular focus on immune checkpoint inhibitors such PD-1 CTLA-4 inhibitors. These have demonstrated substantial efficacy by harnessing the body’s response against tumors. Moreover, adoptive cell transfer (ACT), anti-angiogenic therapy, combination therapies garnered attention their potential in MM treatment. ACT involves modifying T cells target cells, showing antitumor activity. Anti-angiogenic therapy aims impede tumor growth inhibiting angiogenesis, while therapies, including targeted offer multifaceted approach overcome resistance. This comprehensive review explores advancements highlighting role of diverse therapeutic modalities enhancing outcomes addressing challenges posed this malignancy.
Language: Английский
Citations
1
Cancer Management and Research, Journal Year: 2024, Volume and Issue: Volume 16, P. 825 - 839
Published: July 1, 2024
Background: Exosomes contain abundant circular RNAs (circRNAs), playing an important role in intercellular communication. However, the function and underlying molecular mechanism of exosomal circRNAs foot metastatic melanoma remain unclear. Methods: Twelve differentially expressed between patients with primary were screened through high-throughput sequencing, their expression levels detected by real-time reverse transcriptase-polymerase chain reaction (RT-qPCR). CircRNA102927 silencing overexpression A2058 cell line was constructed, effects circRNA102927 on proliferation, apoptosis, migration, invasion, epithelial-mesenchymal transition (EMT) assessed using counting kit-8 (CCK-8), flow cytometry, wound healing, Transwell, Western blot assays, respectively. Results: ROC curve showed that six could be used as diagnostic biomarkers for melanoma. Melanoma-secreted exosomes induced differentiation CD4+ T cells into Treg cells. highly melanomas. Functionally, inhibited EMT, invasion cells, while promoting apoptosis. Meanwhile, had opposite effects. Conclusion: Our investigation suggests may suppress metastasis inhibiting invasiveness, EMT Keywords: circRNAs, CircRNA102927, melanoma,
Language: Английский
Citations
0
Cells, Journal Year: 2024, Volume and Issue: 13(20), P. 1678 - 1678
Published: Oct. 11, 2024
This study evaluated ethosomes as a novel nanodelivery system for nutlin-3a, known MDM2 inhibitor and activator of the p53 pathway, to improve nutlin-3a's poor solubility, limiting its bio-distribution therapeutic efficacy. The potential nutlin-3a-loaded was investigated on two in vitro models melanoma: HT144 cell line
Language: Английский
Citations
0