Journal of Enzyme Inhibition and Medicinal Chemistry, Journal Year: 2024, Volume and Issue: 39(1)
Published: Aug. 29, 2024
An oxidising and substituting one-pot reaction strategy has been developed to synthesise dihydromyricetin derivatives with the aim of enhancing inhibitory activity against SARS-CoV-2. Different
Language: Английский
Microorganisms, Journal Year: 2025, Volume and Issue: 13(1), P. 63 - 63
Published: Jan. 2, 2025
Acute respiratory infections are a significant challenge in primary care and hospital settings. Viruses the most common etiology overlapping symptomatology among major viruses, such as influenza, severe acute syndrome coronavirus 2, syncytial virus, requires use of diagnostic tests that deliver early accurate results. With increasing availability rapid antigen (RATS), it is tempting to prefer them over polymerase chain reaction (PCR) tests. However, compelling arguments support existing recommendations some European countries maintain PCR testing for patient management throughout year. RATs show sensitivities below 30% with lower viral loads, which can have clinical implications. perform well at cycle threshold (Ct) values, sensitivity reaching 97.9% Ct values 20, drops significantly above 25. Factors affecting load include disease stage, vaccination status, variants, all compromise accuracy Multi-target effectively overcome these issues, ensuring reliable diagnosis. Additionally, detection paucisymptomatic cases essential settings facilitate isolation prevent secondary infections. Economic analyses comprehensive tests, triplex-type detecting SARS-CoV-2, influenza RSV, first-line approach, they reduce case numbers healthcare resource utilization. Maintaining year-round therefore crucial effective
Language: Английский
Citations
2
Frontiers in Immunology, Journal Year: 2025, Volume and Issue: 15
Published: Jan. 3, 2025
The unrelenting emergence of SARS-CoV-2 variants has significantly challenged the efficacy existing COVID-19 vaccines. Enhancing stability and immunogenicity spike protein is critical for improving vaccine performance addressing variant-driven immune evasion. We developed an mRNA-based vaccine, RV-1730, encoding Delta variant with S6P mutation to enhance immunogenicity. vaccine's protective were evaluated in preclinical models, including monovalent (RV-1730) bivalent (RV-1731) formulations targeting BA.1 variants. Additionally, effectiveness RV-1730 as a heterologous booster following primary vaccination BNT162b2 (Pfizer-BioNTech) mRNA-1273 (Moderna-NIAID) was assessed. elicited stronger B T cell responses more durable neutralizing antibodies compared S2P-based RV-1731 demonstrated broad activity against emerging variants, XBB1.5 JN.1. Importantly, when used initial immunization or mRNA-1273, enhanced antibody titers multiple Omicron. Both provided superior protection indicating due mutation. incorporation into enhances strong broad-spectrum underscore their potential versatile effective strategies its evolving
Language: Английский
Citations
1
Journal of Medical Virology, Journal Year: 2025, Volume and Issue: 97(2)
Published: Feb. 1, 2025
ABSTRACT Since early 2022 the Omicron variant has rapidly spread worldwide, becoming dominant to date. The study aimed investigate clinical and epidemiological characteristics of COVID‐19 patients reconstruct genomic epidemiology main SARS‐CoV‐2 sublineages in Italy 2022. A total 8970 samples were studied, phylogenetic analyses focused on BA.1, BA.2, BA.5 subvariants. More than half subjects received three doses vaccine experienced a reinfection. significant larger proportion unvaccinated presented reinfection compared with vaccinated. Clusters tMRCA between September–November 2021 (BA.1), November 2021–January (BA.2), October 2021–May (BA.5). R e values showed highest level September–October, January–February 2022, May for BA.2 BA.5, respectively. Limited number studied sequences are included clusters. rate exceeded its evolutionary rate. No single sublineage had sufficient time differentiate into large clusters, but only small fragmented groups sharing same recent ancestor. These dissect dynamics over period great changes epidemic.
Language: Английский
Citations
0
Journal of Proteome Research, Journal Year: 2025, Volume and Issue: 24(2), P. 499 - 514
Published: Jan. 13, 2025
Since late 2021, Omicron variants have dominated the epidemiological scenario as most successful severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) sublineages, driving new and breakthrough infections globally over past two years. In this study, we investigated for first time host salivary response of COVID-19 patients infected with (BA.1, BA.2, BA.4/5) by using an untargeted four-dimensional data-independent acquisition (4D-DIA)-based proteomics approach. We identified 137 proteins whose abundance levels differed between positive negative groups. Salivary signatures were mainly enriched in ribosomal proteins, linked to mRNAviral translation, protein synthesis processing, immune innate, antiapoptotic signaling. The higher 14-3-3 (YWHAG, YWHAQ, YWHAE, SFN) saliva, reported here, may be associated increased infectivity improved viral replicative fitness. also seven (ACTN1, H2AC2, GSN, NDKA, CD109, GGH, PCYOX) that yielded comprehension into infection performed outstandingly screening a hospital setting. This panel presented enhanced anti-COVID-19 anti-inflammatory signature, providing insights disease severity, supported comparisons other proteome data sets. signature provided valuable host's SARS-CoV-2 infection, shedding light on pathophysiology COVID-19, particularly cases mild disease. It underscores potential clinical applications saliva settings. Data are available via ProteomeXchange identifier PXD054133.
Language: Английский
Citations
0
Frontiers in Immunology, Journal Year: 2025, Volume and Issue: 16
Published: Jan. 28, 2025
In this article we discuss characteristics of fusion protein-based SARS-CoV-2 vaccines. We focus on recombinant vaccine antigens comprising proteins consisting combinations SARS-CoV-2-derived or peptides antigens/peptides with SARS-CoV-2-unrelated proteins/peptides. These are made to increase the immunogenicity and/or enable special targeting immune system. The approach is exemplified solely in a proof concept study by using W-PreS-O, chimeric based single protein (W-PreS-O), combining RBDs from Wuhan hu-1 wild-type and Omicron BA.1 hepatitis B virus (HBV)-derived PreS surface antigen adsorbed aluminum hydroxide. W-PreS-O was evaluated Syrian hamsters which were immunized three times at three-week intervals hydroxide (placebo) before they infected BA.1. Neutralizing antibody (nAB) titers, weight, lung symptoms, viral loads, as measured RT-PCR upper lower respiratory tracts, determined. addition, infectious titers lungs plaque-forming assay. found that W-PreS-O-vaccinated developed robust nABs against BA.1, showed almost no development pneumonia, had significantly reduced lungs. Importantly, loads nasal cavities close above PCR cycle threshold considered be non-infectious. data our proof-of-concept provides compelling evidence has protective effect hamster vivo infection model thus support promising results obtained also for other
Language: Английский
Citations
0
International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(17), P. 9498 - 9498
Published: Aug. 31, 2024
The ongoing COVID-19 pandemic, caused by SARS-CoV-2, continues to pose significant global health challenges. results demonstrated that GB-2 at 200 μg/mL effectively increased the population of 293T-ACE2 cells with low RBD binding for both SARS-CoV-2 Omicron EG.5.1 and HV.1 variants dual-color flow cytometry, indicating its ability inhibit virus attachment. Further investigation revealed (+)-catechin 25 50 did not significantly alter ACE2-RBD interaction variant. In contrast, theaflavin showed inhibitory effects EG.5.1, while only higher concentration was effective HV.1. Notably, 3-gallate exhibited a potent inhibition concentrations tested. Molecular docking studies provided insight into mechanisms variants. Both compounds favorable scores, demonstrating slightly lower scores (-8 kcal/mol) compared (-7 These suggest stable interactions between key residues in RBD, potentially explaining their on conclusion, GB-2, theaflavin, demonstrate potential as inhibitors variants, highlighting therapeutic promise against COVID-19. However, these findings are primarily based computational vitro studies, necessitating further vivo research clinical trials confirm efficacy safety humans.
Language: Английский
Citations
2
The Science of The Total Environment, Journal Year: 2024, Volume and Issue: 957, P. 177449 - 177449
Published: Nov. 19, 2024
Wastewater-based epidemiology (WBE) is an effective, non-invasive method for monitoring the spread of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) by tracking viral prevalence in water. This study aimed to investigate presence SARS-CoV-2 surface water Vietnam over two years. One-step quantitative reverse transcription polymerase chain reaction (qRT-PCR) assays were employed quantify and its variant-specific mutation sites (G339D/E484A) pepper mild mottle virus (PMMoV) from a total 315 samples (105 per type) compare with reported Coronavirus disease 2019 (COVID-19) cases environmental factors. was detected 38 % (40/105), 43 (45/105), 39 (41/105) Sites A, B, C, respectively, concentrations 3.0-5.6 log
Language: Английский
Citations
2
Journal of Enzyme Inhibition and Medicinal Chemistry, Journal Year: 2024, Volume and Issue: 39(1)
Published: Aug. 29, 2024
An oxidising and substituting one-pot reaction strategy has been developed to synthesise dihydromyricetin derivatives with the aim of enhancing inhibitory activity against SARS-CoV-2. Different
Language: Английский
Citations
1