Circulating Food Allergen‐Specific Antibodies, Beyond IgG4, Are Elevated in Eosinophilic Esophagitis

Clinical & Experimental Allergy, Journal Year: 2025, Volume and Issue: unknown

Published: April 14, 2025

ABSTRACT Introduction Eosinophilic esophagitis (EoE) is a chronic inflammatory condition with an incompletely understood immuno‐pathogenesis involving T2 response. EoE triggered by food allergens although, unlike IgE‐mediated allergies, it exhibits high IgG4 levels in oesophageal biopsies and circulation. We investigated whether other antibody isotypes specific for are elevated vary disease activity. Methods Plasma samples from patients active ( n = 51), inactive 82) non‐EoE controls 14) were analysed food‐specific IgG IgA subclasses against casein, whey, wheat, egg individual cow's milk ELISA. α‐lactalbumin (Bos d 4)‐ β‐lactoglobulin 5)‐specific B cells measured flow cytometry subset of patients. Results Food allergen‐specific antibodies the plasma varied across subgroups controls. Elevated confirmed strong response to allergens, including wheat egg. α S1 ‐casein 9)‐specific IgG, IgG2, IgG4, IgA1 IgA2 differed between EoE. β‐casein 11, A1 variant) measurements showed higher IgG2 both groups, whereas whey‐derived opposing responses: Bos 4 responses favoured 5 multiple Allergen‐specific could not be isolated Conclusion Our findings reveal distinct profiles plasma, beyond highlighting complex immune allergens. Differential support their clinical relevance dietary management strategies, while absence circulation likely restricts production inflamed oesophagus. Future research should explore these can guide personalised treatment novel therapeutic targets

Language: Английский

Circulating immunome fingerprint in eosinophilic esophagitis is associated with clinical response to proton pump inhibitor treatment

Frontiers in Immunology, Journal Year: 2024, Volume and Issue: 15

Published: April 5, 2024

Objectives The aim of the study was to characterize circulating immunome patients with EoE before and after proton pump inhibitor (PPI) treatment in order identify potential non-invasive biomarkers response. Methods PBMCs from 19 healthy controls 24 were studied using a 39-plex spectral cytometry panel. plasmacytoid dendritic cell (pDC) population differentially characterized by analysis immunofluorescence assays esophageal biopsies 7 13 patients. Results Interestingly, at baseline had lower levels pDC compared controls. Before treatment, who responded PPI therapy higher classical monocytes, non-responders. Moreover, following increased all patients, while normal only restored PPI-responding Finally, inversely correlated peak eosinophil count biopsies. number tissue pDCs significantly during active EoE, being even non-responder when responder pre-PPI. decreased intake, further almost control post-PPI. Conclusions We hereby describe unique immune fingerprint diagnosis. may be also used as novel biomarker predict subsequent response treatment.

Language: Английский