Beating Cardiac Cell Cultures From Different Developmental Stages of Rainbow Trout as a Novel Approach for Replication of Cardiac Fish Viruses

Journal of Fish Diseases, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 17, 2025

ABSTRACT Piscine orthoreovirus‐1 and 3 (PRV‐1, PRV‐3) cause highly prevalent infection in cultured salmonids can induce heart skeletal muscle inflammation (HSMI) resulting economic losses aquaculture. However, to date, PRV‐1 PRV‐3 have withstood replication continuous cell lines. In this study, we used beating cultures obtained from different developmental stages of rainbow trout ( Oncorhynchus mykiss ) (RTC‐L RTC‐A) tested their ability sustain PRV‐3. Furthermore, compared the pattern viruses with those newly developed fibroblast line (RTH‐F) traditional established gonad (RTG‐2). Neither RTCs nor RTH‐F lines supported Comparative experiments showed varying susceptibility novel viral haemorrhagic septicaemia virus (VHSV), chum salmon reovirus (CSV), infectious pancreatic necrosis (IPNV), piscine myocarditis (PMCV), salmonid alphavirus (SAV‐3) tilapia lake (TiLV), indicating usability for work multiple fish viruses. While confirming difficulty replicating PRV‐3, results demonstrate potential heart‐derived as vitro tools studying

Language: Английский

Comparison of transcriptome responses in blood cells of Atlantic salmon infected by three genotypes of Piscine orthoreovirus

Fish & Shellfish Immunology, Journal Year: 2024, Volume and Issue: 157, P. 110088 - 110088

Published: Dec. 9, 2024

Language: Английский

Establishment of a genetically amenable fibroblast cell line from Atlantic Salmon skin.

Experimental Cell Research, Journal Year: 2024, Volume and Issue: 443(1), P. 114295 - 114295

Published: Oct. 22, 2024

Language: Английский