Identification and clinical validation of diverse cell-death patterns-associated prognostic features among low-grade gliomas DOI Creative Commons
Wenyong Yang, Hui Yu,

Qingqiang Lei

et al.

Scientific Reports, Journal Year: 2024, Volume and Issue: 14(1)

Published: May 24, 2024

Low-grade glioma (LGG) is heterogeneous at biological and transcriptomic levels, it still controversial for the definition typing of LGG. Therefore, there an urgent need specific practical molecular signatures accurate diagnosis, individualized therapy, prognostic evaluation Cell death essential maintaining homeostasis, developing preventing hyperproliferative malignancies. Based on diverse programmed cell (PCD) related genes characteristics LGG, this study constructed a model to explore mechanism treatment strategies LGG metastasis invasion. We screened 1161 associated with PCD divided 512 samples into C1 C2 subtypes by consistent cluster analysis. analyzed two subtypes' differentially expressed (DEGs) performed functional enrichment Using R packages such as ESTIMATE, CIBERSOTR, MCPcounter, we assessed immune scores both subtypes. Compared C1, subtype has poor prognosis higher score, patients in are more strongly tumor progression. LASSO COX regression analysis four characteristic (CLU, FHL3, GIMAP2, HVCN1). data sets from different platforms validate four-gene feature, found that expression correlation feature had high degree stability, showing stable predictive effects. Besides, downregulation CLU, GIMAP2 significantly impairs growth, migration, invasive potential cells. Take together, based PCD-related provides valuable information further pathogenesis clinical

Language: Английский

Single-cell multiomic analysis unveils the immune landscape dynamics of graves’ ophthalmopathy DOI Creative Commons

Shurui Ke,

Zhong Liu, Ming Zhou

et al.

Communications Biology, Journal Year: 2025, Volume and Issue: 8(1)

Published: May 12, 2025

Graves' ophthalmopathy (GO) is an autoimmune disease that occurs concurrently with disease, potentially leading to facial disfigurement and irreversible vision loss. However, the molecular characteristics of cellular changes in peripheral immune environment GO patients remain unclear. This study presents a multi-omics single-cell analysis blood from healthy controls, revealing key alterations cell subpopulations. We identify increased chromatin accessibility regulatory activity pro-inflammatory factors, such as FOS NF-κB family. Immune repertoire shows enhanced diversity patients. Notably, GO-specific clonal expansions are enriched CD8 effector T (CD8 Te) cells, which exhibit signs chemotaxis exhaustion. Multi-omics integration reveals SLC35G1 IDNK expression Te linked phenotypes, trends aligning experimental results. find this cluster could infiltrate into orbital tissues upregulate fibrosis-related pathways associated fibroblasts GO. These findings indicate its potential role driving progression. Our epigenetic transcriptomic landscape patients, enhancing understanding pathogenic mechanisms offering directions for prevention treatment.

Language: Английский

Citations

0
Hub genes and key pathways of Graves’ disease: bioinformatics analysis and validation DOI

D. Zhuang,

Xin Hu, Huibin Huang

et al.

HORMONES, Journal Year: 2025, Volume and Issue: unknown

Published: May 19, 2025

Language: Английский

Citations

0
Identification and clinical validation of diverse cell-death patterns-associated prognostic features among low-grade gliomas DOI Creative Commons
Wenyong Yang, Hui Yu,

Qingqiang Lei

et al.

Scientific Reports, Journal Year: 2024, Volume and Issue: 14(1)

Published: May 24, 2024

Low-grade glioma (LGG) is heterogeneous at biological and transcriptomic levels, it still controversial for the definition typing of LGG. Therefore, there an urgent need specific practical molecular signatures accurate diagnosis, individualized therapy, prognostic evaluation Cell death essential maintaining homeostasis, developing preventing hyperproliferative malignancies. Based on diverse programmed cell (PCD) related genes characteristics LGG, this study constructed a model to explore mechanism treatment strategies LGG metastasis invasion. We screened 1161 associated with PCD divided 512 samples into C1 C2 subtypes by consistent cluster analysis. analyzed two subtypes' differentially expressed (DEGs) performed functional enrichment Using R packages such as ESTIMATE, CIBERSOTR, MCPcounter, we assessed immune scores both subtypes. Compared C1, subtype has poor prognosis higher score, patients in are more strongly tumor progression. LASSO COX regression analysis four characteristic (CLU, FHL3, GIMAP2, HVCN1). data sets from different platforms validate four-gene feature, found that expression correlation feature had high degree stability, showing stable predictive effects. Besides, downregulation CLU, GIMAP2 significantly impairs growth, migration, invasive potential cells. Take together, based PCD-related provides valuable information further pathogenesis clinical

Language: Английский

Citations

1