Hepatic conditioning results in better lung endothelial cell preservation under hypoxic environment in vitro

The International Journal of Artificial Organs, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 30, 2025

Background: as we look to extend ex vivo lung perfusion times (EVLP) improve preservation, the metabolic activity of lungs will require support from other organ functions. Active functional liver support, including detoxification, synthesis, and regulation, can preservation during EVLP. This study aimed demonstrate effects hepatic conditioning EVLP perfusate on endothelium, via receptor advanced glycation end-products (RAGE)-nuclear-factor-κB (NF-κB) signaling in vitro. Methods: performed vitro experiments using human microvascular endothelial cells (HLMVECs), hepatocytes, (Steen solution). Four experimental groups: 1) fresh Steen (negative controls, NC), 2) EVLP’ed control, this solution collected after 12 h lungs, 3) hepatocyte conditioned (Hep-cond.), 4) a RAGE inhibitor added (RAGE inhibitor). HLMVECs were incubated each testing condition exposed hypoxia (1% O 2 /8% CO ) for 24 h. Media investigate NF-κB glycocalyx damage. Results: under showed significantly upregulated signal damage denoted by increased glycosaminoglycans matrix metalloproteinase-2 among groups. The Hep-cond. attenuated those findings, while but not Conclusion: Our demonstrates that function incorporated into ameliorate pulmonary injury hypoxic normothermic exposure. data supports concept incorporating functions an platform, enhance graft preservation.

Language: Английский

Identification and validation of biomarkers related to mitochondria during ex vivo lung perfusion for lung transplants based on machine learning algorithm

Gene, Journal Year: 2024, Volume and Issue: 936, P. 149097 - 149097

Published: Nov. 15, 2024

Ex vivo lung perfusion (EVLP) is a critical strategy to rehabilitate marginal donor lungs, thereby increasing transplantation (LTx) rates. Ischemia-reperfusion (I/R) injury inevitably occurs during LTx. Exploring the common mechanisms between EVLP and I/R may unveil new treatment targets enhance LTx outcomes.

Language: Английский