Discovery of Endothelial–Monocyte Crosstalk in Ischemic‐Reperfusion Injury Following Liver Transplantation Based on Integration of Single‐Cell RNA and Transcriptome RNA Sequencing

Journal of Cellular and Molecular Medicine, Journal Year: 2025, Volume and Issue: 29(4)

Published: Feb. 1, 2025

ABSTRACT Hepatic ischemia/reperfusion injury (IRI) commonly complicates liver transplantation (LT). However, the precise mechanisms underlying hepatic IRI remain incompletely understood. We acquired single‐cell RNA sequencing (scRNA‐seq) and transcriptome data of LT patients from GEO database. Employing scRNA‐seq, we delved into interplay between non‐immune immune cells in IRI, pinpointing genes exhibiting altered expression patterns. Integrating insights gleaned scRNA‐seq datasets, deepened our comprehension cellular interactions IRI. Additionally, conducted preliminary validation identified gene alterations using immunofluorescence techniques. Using detected significant changes populations sinusoidal endothelial (LSECs) monocytes after ischemia–reperfusion (IRI). By integrating with bulk data, key dysregulated LSECs (ICAM1, SOCS3, NFKBIZ, JUND, TNFRSF12A HSPA6) (SOCS3, FPR2 NR4A2). Our analysis cell communication indicated that ANXA1‐FPR2 axis might be a pivotal signature mediating monocytes. then established mouse model for further analyses flow cytometry showed increase monocyte proportion post‐IR ( p < 0.01). Consistently, Western Blot also revealed upregulation ANXA1 study elucidated signalling pathways following The likely triggers cascade events, promoting infiltration amplifying inflammatory responses, thus worsening deleterious effects

Language: Английский

Identification of mitophagy-related gene signatures for predicting delayed graft function and renal allograft loss post-kidney transplantation

Transplant Immunology, Journal Year: 2024, Volume and Issue: 87, P. 102148 - 102148

Published: Nov. 14, 2024

Language: Английский

Milk fat globule-epidermal growth factor-VIII–derived oligopeptide 3 (MOP3) attenuates inflammation and improves survival in hepatic ischemia/reperfusion injury

Surgery, Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 1, 2024

Language: Английский