Mucosal Exosome Proteomics of Hybrid Grouper Epinephelus fuscoguttatus♀ × E. lanceolatus♂ Infected by Pseudomonas plecoglossicida DOI Creative Commons
Dong Yang, Xiaowan Ma, Shengping Zhong

et al.

Animals, Journal Year: 2024, Volume and Issue: 14(23), P. 3401 - 3401

Published: Nov. 25, 2024

Pseudomonas plecoglossicida infection, which causes visceral white spot disease, is a significant and economically devastating disease in aquaculture. In this study, we investigated the impact of bacterial infection on protein composition exosomes derived from surface mucus hybrid grouper Epinephelus fuscoguttatus♀ × E. lanceolatus♂. Two hundred healthy fish were randomly separated into challenge control groups. Fish group received 103 CFU/g pathogen P. via intraperitoneal injection, while sterile PBS was used as negative control. After collected extracted for proteomic analysis. The results analysis revealed that significantly increased levels innate immune proteins, including lysosomal peroxisomal within exosomes. Furthermore, CAD found to play pivotal role interaction networks involved response infection. Intriguingly, also observed increase metal-binding proteins exosomes, providing important evidence nutritional immunity surfaces hosts. Notably, several such plasma kallikrein, Annexin A5, eukaryotic translation initiation factor 3 subunit M, S-methyl-5-thioadenosine phosphorylase, exhibited remarkable abundance after These show promising potential noninvasive biomarkers diagnosis disease. study contributes understanding host may aid policymakers implementing appropriate intervention measures effective risk management

Language: Английский

Cooperative Mechanisms of LexA and HtpG in the Regulation of Virulence Gene Expression in Pseudomonas plecoglossicida DOI Creative Commons

Rongchao He,

Yanfei Zuo,

Qiu Li

et al.

Current Research in Microbial Sciences, Journal Year: 2025, Volume and Issue: 8, P. 100351 - 100351

Published: Jan. 1, 2025

Language: Английский

Citations

1
Gut-bacteria derived membrane vesicles and host metabolic health: a narrative review DOI Creative Commons
Jari Verbunt, Johan W. E. Jocken, Ellen E. Blaak

et al.

Gut Microbes, Journal Year: 2024, Volume and Issue: 16(1)

Published: May 29, 2024

The intestinal microbiota, consisting of an estimated 10^10-10^11 organisms, regulate physiological processes involved in digestion, metabolism, and immunity. Surprisingly, these microorganisms have been found to influence tissues that are not directly contact with the gut, such as adipose tissue, liver, skeletal muscle, brain. This interaction takes place even when barrier function is uncompromised. An increasing body evidence suggests bacterial membrane vesicles (bMVs), addition metabolites short-chain fatty acids, able mediate effects microbiota on host tissues. ability bMVs dissipate from lumen into systemic circulation hereby facilitates transport presentation components organs. Importantly, there indications between or immune cells may play a role etiology (chronic metabolic) disease. For example, gut-derived bMV-mediated induction insulin resistance muscle pro-inflammatory signaling by adipocytes possibly underlies diseases type 2 diabetes obesity. Here, we review current knowledge microbiota's energy/substrate metabolism focus etiological roles onset progression metabolic We furthermore illustrate vesicle production could potentially be modulated through lifestyle intervention improve metabolism.

Language: Английский

Citations

4
Innovative Strategies in Oncology: Bacterial Membrane Vesicle-Based Drug Delivery Systems for Cancer Diagnosis and Therapy DOI Creative Commons
Guodong Li, Shiyun Pu, Lingchong You

et al.

Pharmaceutics, Journal Year: 2025, Volume and Issue: 17(1), P. 58 - 58

Published: Jan. 3, 2025

Outer membrane vesicles (OMVs) are double-layered structures of nanoscale lipids released by gram-negative bacteria. They have the same composition and characteristics as primitive cells, which enables them to penetrate cells tissues efficiently. These OMVs exhibit excellent stability, immunogenicity, safety, permeability (which makes it easier for into tumour tissue), making suitable developing cancer vaccines drug delivery systems. Recent studies focused on engineering enhance tumour-targeting capabilities, reduce toxicity, extend circulation time in vivo. This article reviews latest progress OMV treatment discusses challenges associated with use OMV-based antitumour therapy clinical practice.

Language: Английский

Citations

0
Mastitis-related Staphylococcus aureus-derived extracellular vesicles induce a pro-inflammatory response in bovine monocyte-derived macrophages DOI Creative Commons
Mara D. Saenz-de-Juano, Giulia Silvestrelli,

Samuel Buri

et al.

Scientific Reports, Journal Year: 2025, Volume and Issue: 15(1)

Published: Feb. 19, 2025

Abstract Staphylococcus aureus ( S. ) is one of the most common causative agents mammary gland infection and mastitis, but specific role -derived extracellular vesicles (SaEVs) in mastitis has been poorly studied to date. Here, we aimed investigate response bovine monocyte-derived macrophages (boMdM) SaEVs genotype B (GTB) mastitis-related strain M5512B. Specifically, evaluated effects on actin cytoskeleton, gene expression, SaEV proteomic cargo. Furthermore, assessed what extent cellular molecular boMdM differed from peripheral mononuclear blood cells (PBMCs) used for vitro derivation former. We observed that induced morphological changes boMdM, leading a pro-inflammatory pyroptosis-related increased expression. Additionally, our study revealed PBMCs exhibited stimulus-specific differing responses. The analysis identified clusters proteins related virulence antibiotic resistance, supporting theory might use EVs evade host defences colonize gland. Our results bring new insights into how impact during an infection, which can be useful future vaccine development.

Language: Английский

Citations

0
Host-dependent alteration of the gut microbiota: the role of luminal microRNAs DOI Open Access

Céline Cuinat,

Jianlin Pan,

Elena M. Comelli

et al.

Microbiome Research Reports, Journal Year: 2025, Volume and Issue: 4(1)

Published: Feb. 21, 2025

MicroRNAs (miRNAs) are short, non-coding RNAs that play gene expression regulatory roles in eukaryotes. MiRNAs also released body fluids, and the intestine, they found lumen feces. Here, together with exogenous dietary-derived miRNAs, constitute fecal miRNome. Several miRNAs were identified feces of healthy adults, including, as shown here, core hsa-miR-21-5p hsa-miR-1246. These important for intestinal homeostasis. Recent evidence suggests may interact gut bacteria. This represents a new avenue to understand host-bacteria crosstalk its role health disease. review provides comprehensive overview current knowledge on their representation across individuals, effects microbiota. It discusses existing potential mechanisms uptake interaction bacterial genomes, drawing from prokaryotic small (sRNAs) regulation expression. Finally, we silico experimental approaches profiling miRNA-mRNA interactions species, highlighting challenges target validation. work emphasizes need further research into host miRNA-bacterial better ecosystem support exploitation disease prevention treatment.

Language: Английский

Citations

0
Outer membrane vesicles in gram-negative bacteria and its correlation with pathogenesis DOI Creative Commons
F Abolhasani,

Nasim Vaghefinanekaran,

Aref Yarahmadi

et al.

Frontiers in Immunology, Journal Year: 2025, Volume and Issue: 16

Published: April 1, 2025

There is a widespread distribution of gram-negative bacteria worldwide, which are responsible for the deaths numerous patients each year. The illnesses they cause can be localized and systemic, these possess several key virulence factors that contribute to their pathogenicity. In recent years, distinct mechanisms pathogenesis have evolved remain largely unknown scientists medical experts. Among these, outer membrane vesicles (OMVs) undoubtedly one most significant influencing virulence. OMVs contain various bacterial compounds diverse effects on host organisms immune system, potentially exacerbating disease inflammation while evading responses. This review comprehensively examines role in pathogenesis, interaction with cells, potential biomedical applications. Understanding molecular governing OMV biogenesis function could pave way novel antimicrobial strategies therapeutic interventions.

Language: Английский

Citations

0
Algicidal bacteria-derived membrane vesicles as shuttles mediating cross-kingdom interactions between bacteria and algae DOI Creative Commons
Yixin Li, Yuezhou Wang, Xiaolan Lin

et al.

Science Advances, Journal Year: 2024, Volume and Issue: 10(32)

Published: Aug. 7, 2024

Bacterial membrane vesicles (BMVs) are crucial biological vehicles for facilitating interspecies and interkingdom interactions. However, the extent mechanisms of BMV involvement in bacterial-algal communication remain elusive. This study provides evidence BMVs delivering cargos to targeted microalgae. Membrane (MVs) from Chitinimonas prasina LY03 demonstrated an algicidal profile similar strain LY03. Further investigation revealed Tambjamine LY2, effective compound, selectively packaged into LY03-MVs. Microscopic imaging efficient delivery LY2 microalgae Heterosigma akashiwo Thalassiosira pseudonana through fusion. In addition, versatile cargo capabilities algae, including transfer MV-carried nucleic acids algal cells revival growth iron-depleted by MVs. Collectively, our findings reveal a previously unknown mechanism which bacteria store hydrophobic compounds MVs trigger target death highlight potency understanding engineering bacterial-algae cross-talk.

Language: Английский

Citations

0
Contact-Mediated Algicidal Mechanism of Vibrio coralliirubri ACE001 Against the Harmful Alga Karenia mikimotoi DOI Creative Commons

Jiaying Yu,

Wei Xu, Jiaxin Wang

et al.

iScience, Journal Year: 2024, Volume and Issue: 27(11), P. 111254 - 111254

Published: Oct. 30, 2024

Language: Английский

Citations

0
Mucosal Exosome Proteomics of Hybrid Grouper Epinephelus fuscoguttatus♀ × E. lanceolatus♂ Infected by Pseudomonas plecoglossicida DOI Creative Commons
Dong Yang, Xiaowan Ma, Shengping Zhong

et al.

Animals, Journal Year: 2024, Volume and Issue: 14(23), P. 3401 - 3401

Published: Nov. 25, 2024

Pseudomonas plecoglossicida infection, which causes visceral white spot disease, is a significant and economically devastating disease in aquaculture. In this study, we investigated the impact of bacterial infection on protein composition exosomes derived from surface mucus hybrid grouper Epinephelus fuscoguttatus♀ × E. lanceolatus♂. Two hundred healthy fish were randomly separated into challenge control groups. Fish group received 103 CFU/g pathogen P. via intraperitoneal injection, while sterile PBS was used as negative control. After collected extracted for proteomic analysis. The results analysis revealed that significantly increased levels innate immune proteins, including lysosomal peroxisomal within exosomes. Furthermore, CAD found to play pivotal role interaction networks involved response infection. Intriguingly, also observed increase metal-binding proteins exosomes, providing important evidence nutritional immunity surfaces hosts. Notably, several such plasma kallikrein, Annexin A5, eukaryotic translation initiation factor 3 subunit M, S-methyl-5-thioadenosine phosphorylase, exhibited remarkable abundance after These show promising potential noninvasive biomarkers diagnosis disease. study contributes understanding host may aid policymakers implementing appropriate intervention measures effective risk management

Language: Английский

Citations

0