Boosting human immunology: harnessing the potential of immune organoids

EMBO Molecular Medicine, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 27, 2025

Language: Английский

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Functional organotypic human lymph node model with native immune cells benefits from fibroblastic reticular cell enrichment

Scientific Reports, Journal Year: 2025, Volume and Issue: 15(1)

Published: April 10, 2025

Lymphoid organ function depends on fibroblastic reticular cells (FRCs), the non-hematopoietic mesenchymal stromal that crucially support immune activity in human lymph nodes (LNs). The vitro study of immunology requires physiological LN models, yet inclusion FRCs current models is lacking. Here, we created an organotypic hydrogel model, containing native from tissue and ex vivo cultured autologous FRCs. During a oneweek culture period, enrichment into model benefited viability all cell populations, particularly B cells, promoted presence certain subsets including CD4+ naïve T unswitched (US) memory cells. enhanced production immune-related cytokines chemokines, such as activating factor TNF family (BAFF), CXC motif chemokine ligand 12 (CXCL12), CC 19 (CCL19) interleukin-6 (IL-6). Functionality was assessed through activation by CD3 stimulation or initiation allogenic reaction with different maturation statuses monocyte-derived dendritic (moDCs). Interestingly, expansion restricted FRC-enriched reflecting intrinsic characteristic As such, this highlights influence allows opportunity to further antigen-induced responses, e.g. vaccine immunotherapy testing.

Language: Английский

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Human tonsil organoids reveal innate pathways modulating humoral and cellular responses to ChAdOx1

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Oct. 11, 2024

ABSTRACT The COVID-19 pandemic response demonstrated the effectiveness of adenovirus vector vaccines in inducing protective cellular and antibody responses. However, we still lack mechanistic understanding factors regulating immunity induced by this platform, especially innate pathways. We utilized a human tonsil organoid model to study regulation adaptive responses ChAdOx1 nCoV-19. Innate activation cytokine release occurred within 24 hours T B cell antigen-specific secretion during ensuing 14-day culture. Among immune populations, plasmacytoid dendritic cells (pDCs) exhibited highest transduction levels. pDC-derived IFN-α was critical for humoral responses, but production antigen pDCs dispensable. Furthermore, IL-6 enhanced both IFN-α-dependent independent manners, indicating intricate signaling interplay. also regulated function vaccine-activated CD4 + cells, including FH . These data provide key insights into pathways ChAdOx1-induced highlights promise vaccine platform studies. SUMMARY Despite success vaccines, mechanisms platform. This organoids immunity.

Language: Английский

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Mouse Corneal Immune Cell Heterogeneity Revealed by Single-Cell RNA Sequencing

Investigative Ophthalmology & Visual Science, Journal Year: 2024, Volume and Issue: 65(12), P. 29 - 29

Published: Oct. 21, 2024

This study aimed to define the heterogeneity, spatial localization, and functional roles of immune cells in mouse cornea using single-cell RNA sequencing (scRNA-seq) immunofluorescent staining.

Language: Английский

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Immunological characterization of pleural effusions in pediatric patients

Frontiers in Immunology, Journal Year: 2024, Volume and Issue: 15

Published: Dec. 16, 2024

The pleural cavity represents a unique immunological compartment that can mount inflammatory reactions during infections, after surgery and in chronic diseases. connection between systemic immune the blood local effusions remains unclear. This study provides first comprehensive characterization of paired effusion samples, utilizing combined cell cytokine analyses pediatric patients undergoing cardiac surgery.

Language: Английский

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