Key Considerations on CITE‐Seq for Single‐Cell Multiomics

PROTEOMICS, Journal Year: 2025, Volume and Issue: unknown

Published: Feb. 9, 2025

CITE-Seq (Cellular Indexing of Transcriptomes and Epitopes by Sequencing) is an advanced single-cell sequencing method to profile both gene expression protein abundance simultaneously in individual cells using mRNA techniques alongside antibody-derived tags (ADTs), for detection. The characterization the transcriptome proteome from same provides a powerful multiomic approach understanding mechanisms complex biological processes. This review focuses on workflow microwell-based analysis system as example key considerations staining with ADTs. By highlighting critical information library preparation, sequencing, data analysis, this practical guide which perform comprehensive workflow.

Language: Английский

OMIP‐105: A 30‐color full‐spectrum flow cytometry panel to characterize the immune cell landscape in spleen and tumor within a syngeneic MC‐38 murine colon carcinoma model

Cytometry Part A, Journal Year: 2024, Volume and Issue: 105(9), P. 659 - 665

Published: Aug. 6, 2024

This panel was designed to characterize the immune cell landscape in mouse tumor microenvironment as well lymphoid tissues (e.g., spleen). As an example, using MC-38 syngeneic model, we demonstrated that could measure frequency and functional status of CD4 T cells, CD8 regulatory NK B macrophages, granulocytes, monocytes, dendritic cells. is especially useful for understanding "cold" preclinical models with very low infiltration investigating how therapeutic treatments may modulate landscape.

Language: Английский

Optimized full-spectrum flow cytometry panel for deep immunophenotyping of murine lungs

Cell Reports Methods, Journal Year: 2024, Volume and Issue: unknown, P. 100885 - 100885

Published: Oct. 1, 2024

Language: Английский