Biochemical, Histological, and Multi-Omics Analyses Reveal the Molecular and Metabolic Mechanisms of Cold Stress Response in the Chinese Soft-Shelled Turtle (Pelodiscus sinensis)

Biology, Journal Year: 2025, Volume and Issue: 14(1), P. 55 - 55

Published: Jan. 11, 2025

The Chinese soft-shelled turtle (Pelodiscus sinensis), a type of warm-water reptile, is frequently chosen as the model animal to understand how organisms respond environmental stressors. However, responsive mechanism P. sinensis natural cold stress unclear, especially in terms metabolic pattern and molecular pathways. Herein, plasma biochemical, hepatic morphological, apoptotic, transcriptomic, metabolomic detection methods were performed investigate response acute stress. A consistent increase AST ALT activities with decline ALP activity was found following 14 °C 7 compared control group. Plasma GLU, TG, CHO, HDL contents, reflecting energy metabolism, decreased lower levels from 2 16 days post (dps). Histological TUNEL liver demonstrated that caused severe morphological damage cell apoptosis time-dependent manner. DEGs biosynthesis fatty acids (Acsbg2, Acsl3, Acsl4, Acsl5, Mcat, Acacb), well unsaturated (Hsd17b12, Elovl7, Scd, Baat), starch sucrose metabolism (Pgm1, Pgm2, Treh), (Ddit3, Gadd45a, Lmnb1, Tuba1c, Tnf, Tnfsf10, Fos, Itpr1, Ctso) discovered transcriptome under data showed metabolites, including chenodeoxycholic acid, oleoylethanolamide, uric fructose 1,6-bisphosphate, CMP, S-(Hydroxymethyl)-glutathione, remarkably altered groups. Combined transcriptomic revealed pyrimidine amino acid pyruvate most significant pathways regulated by low-temperature exposure. Overall, this work suggests could induce obvious at 4 dps. Moreover, main signaling for sinensis.

Language: Английский

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Infectious diseases of the Chinese soft-shelled turtle (Pelodiscus sinensis): A focus on etiological agents and predisposing factors

Aquaculture Reports, Journal Year: 2024, Volume and Issue: 37, P. 102227 - 102227

Published: July 1, 2024

Language: Английский

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Integrated transcriptomics and metabolomics uncover metabolites and pathways in American bullfrog (Aquarana catesbeiana) response to Elizabethkingia miricola infection

Aquaculture, Journal Year: 2025, Volume and Issue: unknown, P. 742262 - 742262

Published: Feb. 1, 2025

Language: Английский

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Effects of Dietary Supplement of Probiotic Enterococcus faecium on Intestinal Microbiota and Barrier Structure, Immune Function, and Antioxidant Capacity of Soft‐Shelled Turtle Pelodiscus sinensis

Aquaculture Nutrition, Journal Year: 2025, Volume and Issue: 2025(1)

Published: Jan. 1, 2025

Enterococcus faecium inhabits animal gastrointestinal tracts and has been demonstrated to benefit livestock poultry, but its effects on soft‐shelled turtles remain unexplored. The present work investigates the of probiotic E. intestinal microbiota barrier structure, immune function, antioxidant capacity turtle. Twenty‐four juvenile Pelodiscus sinensis were divided into two groups: control (fed a basal diet) treatment diet supplemented with , 3.3 × 10 8 CFU/g feed), over period 6 weeks. We found that did not promote growth at feeding level, resulted in significant alterations microbial community increased abundance Romboutsia Clostridium_sensu_stricto_1 reduction Aeromonas ( p < 0.05). notably enhanced villus height/crypt depth, width, density intestine. group exhibited 1.50‐fold increase goblet cells count 1.18‐fold higher muscular layer thickness compared group. also improved an ratio plasma neutrophils lymphocytes total number leukocytes after probiotics, upregulation levels toll‐like receptor 4 (TLR 4), lysozyme, interleukin 1 β (IL‐1 ), tumor necrosis factor α (TNF‐ immunoglobulin A (IgA) intestine, as well level hepatic M (IgM). Additionally, supplementation boosted capabilities, including catalase (CAT) glutathione peroxidase (GPx) activity reduced (GSH) intestine GSH spleen. Our study demonstrates beneficial supplemental overall health turtles, particularly enhancing their function capacity.

Language: Английский

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Dietary Tannic Acid Improves Hepatic Health and Capacity to Deal with Temperature Fluctuation in the Chinese Soft-Shelled Turtle (Pelodiscus sinensis)

Animals, Journal Year: 2025, Volume and Issue: 15(4), P. 544 - 544

Published: Feb. 13, 2025

To assess the impact of tannic acid (TA) on hepatic health Chinese soft-shelled turtle, individuals were fed diets containing 0 (CG), 0.5, 1, 2, and 4 g/kg TA for 60 days (0 hps). Afterwards, turtles challenged with 15 °C cold stress 24 h (24 hps) then recovered to 28 (48 The results indicated that has a significant toxic effect after 60-day administration. T-SOD, CAT, GSH-Px, T-AOC activities in TA2 increased at hps (p < 0.05) compared CG. Similarly, Sod1, Sod2, Cat, Gsh-px3, Gsh-px4 mRNA levels higher than other four groups 0.05). histology data showed induced injuries liver temperature fluctuation, whereas 2 effectively attenuated injuries. A total 202 differentially expressed metabolites (DEMs) CG vs. 115 DEMs LTCG LTTA2 separately detected by metabolome. cephalosporin C, protoporphyrin, bis(2-ethylhexyl) phthalate, 2-acetamidoethylphosphonat, verbasosid, norcocain, obvious TA2. Furthermore, valienone 7-phosphate, 5-HETE, pregnanetriolone, 4-keto-anhydrotetracyclin, dephospho-CoA, lysoPC(18:4(6Z,9Z,12Z,15Z)/0:0) top LTTA2. "adipocytokine signaling pathway" "AMPK key pathways enriched TA2, while "fatty biosynthesis", "steroid "linoleic metabolism" most abundant Generally, this research could protect from fluctuations improving antioxidant capacity, reducing histological injuries, regulating lipid-related pathways.

Language: Английский

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Molecular Response and Metabolic Reprogramming of the Spleen Coping with Cold Stress in the Chinese Soft-Shelled Turtle (Pelodiscus sinensis)

Antioxidants, Journal Year: 2025, Volume and Issue: 14(2), P. 217 - 217

Published: Feb. 14, 2025

The Chinese soft-shelled turtle (Pelodiscus sinensis), as a type of warm-water reptile, could be induced to massive death by sharp temperature decline. Hence, the mechanism spleen tissue responding cold stress in P. sinensis was investigated. present results showed that superoxide dismutase (SOD) activity declined from 4 16 days post-cold-stress (dps), while catalase (CAT) and glutathione peroxidase (GSH-Px) activities increased, 8 dps 14 °C (T14) 7 (T7) groups. transcriptome T7 group control (CG) at obtained 2625 differentially expressed genes (DEGs), including 1462 upregulated 1663 downregulated genes. DEGs were enriched mainly pathways "intestinal immune network for IgA production" (Pigr, Il15ra, Tnfrsf17, Aicda, Cd28), "toll-like receptor signaling pathway" (Mapk10, Tlr2, Tlr5, Tlr7, Tlr8), "cytokine-cytokine interaction" (Cx3cl1, Cx3cr1, Cxcl14, Cxcr3, Cxcr4). metabolomic data esculentic acid, tyrosol, diosgenin, heptadecanoic 7-ketodeoxycholic acid obviously baccatin III, taurohyocholate, parthenolide, enterolactone, tricin decreased, CG vs. comparison. Integrated analysis two omics revealed "glycine, serine threonine metabolism", "FoxO pathway", "neuroactive ligand-receptor main stress. Overall, this work found low remarkably influenced antioxidant enzyme activities, gene expression pattern, metabolite profile spleen, indicating immunity might weakened sinensis.

Language: Английский

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Global changes in gene expression of mRNA and miRNA in liver tissues of Micropterus salmoides after infection with Aeromonas hydrophila

Aquaculture Reports, Journal Year: 2025, Volume and Issue: 42, P. 102756 - 102756

Published: March 21, 2025

Language: Английский

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Effects of a Chinese herbal medicine mixture on growth performance, immune response, and gene expression in Chinese soft-shelled turtles (Pelodiscus sinensis)

Animal Feed Science and Technology, Journal Year: 2024, Volume and Issue: 316, P. 116084 - 116084

Published: Aug. 10, 2024

Language: Английский

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Comparative Analysis of Intestinal Histology, Antioxidant Activity, Nonspecific Immunity, Gut Microbiota and Meat Quality in Chinese Soft-Shell Turtles Across Different Growth Stages and Physiological States in Greenhouse

Published: Jan. 1, 2024

Language: Английский

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