KLF4: a multifunctional nexus connecting tumor progression and immune regulation

Frontiers in Immunology, Journal Year: 2025, Volume and Issue: 16

Published: Feb. 6, 2025

Krüppel-like factors (KLFs) regulate various biological processes such as cell proliferation, migration, invasion, and differentiation gene transcription factors. Signaling pathways which mediated by KLF4 have a sophisticated role in tumors due to multiple factors, including the types or stage of tumors. plays promoter tumorigenesis development, tumor suppressor context-dependent anti- pro-inflammatory factor. over-expression increases CD8+T enhances antitumor immunity. This review aims provide information about relationship immunity with guide future study.

Language: Английский

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Purine metabolism-associated key genes depict the immune landscape in gout patients

Discover Oncology, Journal Year: 2025, Volume and Issue: 16(1)

Published: Feb. 17, 2025

Language: Английский

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YTHDF3 modulates the progression of breast cancer cells by regulating FGF2 through m6A methylation

Frontiers in Cell and Developmental Biology, Journal Year: 2024, Volume and Issue: 12

Published: Sept. 20, 2024

Introduction Breast cancer (BC) is a prevailing malignancy among women, and its inconspicuous development contributes significantly to mortality. The RNA N6-methyladenosine (m 6 A) modification represents an emerging mechanism for gene expression regulation, with the active involvement of YTH binding protein 3 (YTHDF3) in tumor progression across multiple types. Nonetheless, precise function breast necessitates further investigation. Methods YTHDF3 both cell lines patient tissues was examined using Western blotting, reverse transcription quantitative PCR (RT-qPCR), immunohistochemistry (IHC) techniques. Bioinformatics analysis methylated immunoprecipitation sequencing (MeRIP-seq) transcriptome (RNA-seq) data employed screen target genes YTHDF3. main focus this study investigate vitro biological functions specific correlation m A methylation were studied through pull-down, immunoprecipitation, co-immunoprecipitation experiments. regulatory mechanisms downstream assessed stability analysis. Furthermore, cells validated CRISPR-Cas9 technology rescue Results By constructing risk model TCGA database, identified as high-risk factor factors. Subsequent investigations revealed elevated various subtypes cancer, accompanied by poor prognosis. MeRIP-seq fibroblast growth 2 (FGF2) Knockdown led significant inhibition self-renewal, migration, invasion abilities . Mechanistically, specifically recognized transcript FGF2 within coding sequence (CDS) region, leading degradation. Moreover, depletion markedly suppressed cells, while reducing YTHDF3-overexpressing substantially alleviated malignant progression. Conclusions In summary, our elucidates role oncogene maintaining BC A-dependent mechanism. Additionally, we provide potential biomarker panel prognostic prediction BC.

Language: Английский

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YTHDC1 Regulates the Migration, Invasion, Proliferation, and Apoptosis of Rheumatoid Fibroblast-Like Synoviocytes

Frontiers in Immunology, Journal Year: 2024, Volume and Issue: 15

Published: Oct. 29, 2024

Rheumatoid arthritis (RA), a chronic autoimmune condition, is characterized by persistent synovial inflammation, bone degradation, and progressive joint deterioration. Despite considerable research efforts, the precise molecular mechanism underlying RA remains elusive. This investigation aims to elucidate potential role of N6-methyladenosine (m

Language: Английский

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