RNA sequencing comparing centenarian and middle‐aged women lymphoblastoid cell lines identifies age‐related dysregulated expression of genes encoding selenoproteins, heat shock proteins, CD99, and BID DOI Creative Commons
Irena Voinsky,

Ofir Goldenberg‐Bogner,

Ifat Israel‐Elgali

et al.

Drug Development Research, Journal Year: 2024, Volume and Issue: 85(7)

Published: Oct. 24, 2024

Abstract Women typically live longer than men, and constitute the majority of centenarians. We applied RNA‐sequencing (RNA‐seq) blood‐derived lymphoblastoid cell lines (LCLs) from women aged 60‐80 years centenarians (100‐105 years), validated RNA‐seq findings by real‐time PCR, additionally measured differentially expressed genes in LCLs young 20‐35 years. Top with differential expression between age groups included three selenoproteins ( GPX1, SELENOW, SELENOH ) heat shock proteins HSPA6, HSPA1A, HSPA1B ), highest women, indicating that are better protected oxidative stress. The two additional genes, BID encoding BH3‐interacting domain death agonist CD99 antigen, showed unique dependence, similar levels centenarian while exhibiting higher lower levels, respectively, compared other groups. This age‐related suggests elevated inflammation susceptibility middle‐aged either or women. Our findings, once human peripheral blood mononuclear cells further types, may lead to novel healthy aging diagnostics therapeutics.

Language: Английский

Cellular senescence in the tumor with a bone niche microenvironment: friend or foe? DOI Creative Commons

Sajad Alavimanesh,

Negar Nayerain Jazi,

Maedeh Choubani

et al.

Clinical and Experimental Medicine, Journal Year: 2025, Volume and Issue: 25(1)

Published: Jan. 23, 2025

Cellular senescence is understood to be a biological process that defined as irreversible growth arrest and was originally recognized tumor-suppressive mechanism prevents further propagation of damaged cells. More recently, cellular has been shown have dual role in prevention tumor promotion. Senescent cells carry senescence-associated secretory phenotype (SASP), which altered by factors including pro-inflammatory cytokines, chemokines, other proteases, leading the alteration tissue microenvironment. Though would eventually halt cancerous potential cells, SASP contributes environment promoting inflammation, matrix remodeling, cell invasion. The paradox prevention/promotion particularly relevant bone niche microenvironment, where longer-lasting, chronic inflammation promotes formation. Insights into mechanistic understanding provide basis for targeted therapies, such senolytics, aim eliminate senescent or inhibitors, tumor-promoting effects senescence. These therapeutic interventions offer significant clinical implications treating cancer healthy aging.

Language: Английский

Citations

2
Neuroimmune crosstalk in chronic neuroinflammation: microglial interactions and immune modulation DOI Creative Commons

Ludmila Müller,

Svetlana Di Benedetto

Frontiers in Cellular Neuroscience, Journal Year: 2025, Volume and Issue: 19

Published: April 7, 2025

Neuroinflammation is a fundamental feature of many chronic neurodegenerative diseases, where it contributes to disease onset, progression, and severity. This persistent inflammatory state arises from the activation innate adaptive immune responses within central nervous system (CNS), orchestrated by complex interplay resident cells, infiltrating peripheral an array molecular mediators such as cytokines, chemokines, extracellular vesicles. Among CNS-resident microglia play role, exhibiting dynamic spectrum phenotypes ranging neuroprotective neurotoxic. In sustained microglial often leads amplification cascades, reinforcing pathogenic cycle immune-mediated damage. Intercellular communication inflamed CNS persistence progression neuroinflammation. Microglia engage in extensive crosstalk with astrocytes, neurons, oligodendrocytes, shaping both local systemic responses. These interactions influence key processes synaptic pruning, phagocytosis, blood-brain barrier integrity, cytokine-mediated signaling. Understanding mechanisms cell-cell signaling this context critical for identifying therapeutic strategies modulate response restore homeostasis. review explores players neuroinflammation, focus on role microglia, pathways underlying intercellular communication, potential approaches mitigate neuroinflammatory damage diseases.

Language: Английский

Citations

0
Immunosenescence in digestive system cancers: mechanisms, research advances, and therapeutic strategies DOI

Junyan Zhang,

Xiaojiao Guan,

Xinwen Zhong

et al.

Seminars in Cancer Biology, Journal Year: 2024, Volume and Issue: 106-107, P. 234 - 250

Published: Nov. 1, 2024

Language: Английский

Citations

2
Antioxidant Features of Humic Products by ABTS Assay DOI
Mariavittoria Verrillo, Vincenza Cozzolino, Riccardo Spaccini

et al.

Methods in molecular biology, Journal Year: 2024, Volume and Issue: unknown, P. 223 - 227

Published: Sept. 30, 2024

Language: Английский

Citations

1
RNA sequencing comparing centenarian and middle‐aged women lymphoblastoid cell lines identifies age‐related dysregulated expression of genes encoding selenoproteins, heat shock proteins, CD99, and BID DOI Creative Commons
Irena Voinsky,

Ofir Goldenberg‐Bogner,

Ifat Israel‐Elgali

et al.

Drug Development Research, Journal Year: 2024, Volume and Issue: 85(7)

Published: Oct. 24, 2024

Abstract Women typically live longer than men, and constitute the majority of centenarians. We applied RNA‐sequencing (RNA‐seq) blood‐derived lymphoblastoid cell lines (LCLs) from women aged 60‐80 years centenarians (100‐105 years), validated RNA‐seq findings by real‐time PCR, additionally measured differentially expressed genes in LCLs young 20‐35 years. Top with differential expression between age groups included three selenoproteins ( GPX1, SELENOW, SELENOH ) heat shock proteins HSPA6, HSPA1A, HSPA1B ), highest women, indicating that are better protected oxidative stress. The two additional genes, BID encoding BH3‐interacting domain death agonist CD99 antigen, showed unique dependence, similar levels centenarian while exhibiting higher lower levels, respectively, compared other groups. This age‐related suggests elevated inflammation susceptibility middle‐aged either or women. Our findings, once human peripheral blood mononuclear cells further types, may lead to novel healthy aging diagnostics therapeutics.

Language: Английский

Citations

0