Evaluating antibacterial and antioxidant properties of sericin recovered from cocoons of Bombyx mori, Gonometa postica and Samia ricini in Kenya

PLoS ONE, Journal Year: 2024, Volume and Issue: 19(12), P. e0316259 - e0316259

Published: Dec. 31, 2024

Microbial infections and excessive reactive oxygen species are the primary contributors to delays in wound healing with Pseudomonas aeruginosa, Escherichia coli Staphylococcus aureus as common infection causing bacteria. In fact, management has become more challenging since most of these microbes have developed resistance against commonly used conventional antibiotics thus making it necessary develop natural products both antibacterial antioxidant activities. Increasing attention been paid silk sericin last decade, limited research focus Africa. Therefore, this work on evaluating capacity recovered from cocoons domesticated (Bombyx mori, Samia ricini) wild (Gonometa postica) silkworms Kenya. Sericin recovery was achieved using high temperature-high pressure method. Results revealed significance interspecies variation all parameters. Total flavonoid content ranged between 270±60.1 603.3±44.1 mg GAE/100g S. ricini demonstrating highest whereas G. postica exhibited least content. Moreover, showed total phenolic at 780.0±67.6 QE/100g while had 330.6±14.6 QE/100g. radical scavenging 40.47 ± 3.76% B. mori extract ability 24.6± 2.96%. Furthermore, demonstrated inhibitory activity aureus, aeruginosa Klebsiella pneumonia which translated 70.79 11.28%, 93.86 1.92%, 94.77 7.07% when compared streptomycin, chloramphenicol oxytetracycline respectively. Bombyx Gonometa pyogene E. These findings uncovered sufficient efficacy three extracts Gram-positive negative bacteria, however, depth is still required guarantee aforementioned bioactivities boost therapeutic potential sericin-based biomaterials.

Language: Английский

---

Evaluating research waste and traits among randomized controlled trials of scars over the past 20 years: a cross-sectional study

Postgraduate Medical Journal, Journal Year: 2024, Volume and Issue: 100(1190), P. 925 - 931

Published: July 10, 2024

To analyze the changes in characteristics of randomized controlled trials (RCTs) field scarring over last two decades, unveil components research waste (RW) within these RCTs, and identify targets for improvement.

Language: Английский

---

Hibiscus schizopetalus boosts wound healing via restoring redox balance and hindering inflammatory responses in rats: Insights on metabolome profiling and molecular docking

Archiv der Pharmazie, Journal Year: 2024, Volume and Issue: unknown

Published: Sept. 6, 2024

Abstract Hibiscus species (Malvaceae) possess a plethora of appealing pharmacological activities with an extended history customary use in diverse medical conditions. The present study aimed at comparing the metabolomic analyses three native to Egypt, namely H. tiliaceus , schizopetalus extract (HSE), and rosa‐sinensis alongside identifying promising natural wound healing candidate. Chemical profiling leaf extracts was achieved via UPLC‐ESI/MS/MS‐guided analysis that resulted tentative identification total 48 secondary metabolites pertaining phenolic acids, flavonoids, anthocyanins, fatty amides. Remarkably, vitro studies revealed HSE exhibited topmost activity. Subsequently, formulated into hydro‐ nanogel (1% w/v) formulations for further assessing its efficacy excision model. HSE‐nanogel demonstrated significant vivo contraction activity improving histopathological abnormalities. Mechanistically, upregulated antioxidant status through increasing levels reduced glutathione (GSH) catalase Moreover, suppressed inflammatory responses by diminishing expressions NF‐ĸB, TNF‐α, IL‐6. Molecular docking were performed on HSE's major constituents using CDOCKER, which supported findings. Collectively, exhibits notable aptitude as wound‐healing agent, warranting clinical appraisal.

Language: Английский

---

Wound Closure Promotion by Leucine-Based Pseudo-Proteins: An In Vitro Study

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(17), P. 9641 - 9641

Published: Sept. 6, 2024

Our research explores leucine-based pseudo-proteins (LPPs) for advanced wound dressings, focusing on their effects healing in an vitro model. We assessed three types of LPP films ability to enhance closure rates and modulate cytokine production. They all significantly improved compared traditional methods, with the 8L6 copolymer showing most pronounced effects. Notably, latter exhibited optimal profile: initial burst pro-inflammatory TNF-α, followed by a controlled release IL-6 during proliferative phase significant increase anti-inflammatory IL-10 remodeling. This balanced response suggests that film not only accelerates but also supports well-regulated process, potentially reducing fibrosis abnormal scarring, underscoring potential LPPs as dressing materials. Future will aim elucidate specific signaling pathways activated better understand its mechanism action. Overall, offer promising approach improving care could lead more effective treatments complex wounds.

Language: Английский

---

The Immobilization of an FGF2-Derived Peptide on Culture Plates Improves the Production and Therapeutic Potential of Extracellular Vesicles from Wharton’s Jelly Mesenchymal Stem Cells

International Journal of Molecular Sciences, Journal Year: 2024, Volume and Issue: 25(19), P. 10709 - 10709

Published: Oct. 4, 2024

The skin is an essential organ that protects the body from external aggressions; therefore, damage various wounds can significantly impair its function, and effective methods for regenerating restoring barrier function are crucial. This study aimed to mass-produce wound-healing exosomes using a fragment of fibroblast growth factor 2 (FGF2)-derived peptide (FP2) enhance cell proliferation exosome production. Our experiments demonstrated increased when Wharton’s jelly mesenchymal stem cells (WJ MSCs) were coated with FP2. Exosomes FP2-coated WJ MSCs analyzed nanoparticle-tracking analysis, transmission electron microscopy, Western blotting. Subsequently, fibroblasts treated these exosomes, their viability migration effects compared. Anti-inflammatory also evaluated by inducing pro-inflammatory factors in RAW264.7 cells. treatment MSC-derived (FP2-exo) expression FGF2, confirming effect vivo. Overall, results this highlight significant impact FP2 on anti-inflammatory suggesting potential applications beyond wound healing.

Language: Английский

---