Cited by The innate immune response is a key player in the process of coronary artery and aortic valve calcification

Interferon-gamma receptor signaling regulates innate immunity during Staphylococcus aureus craniotomy infection DOI

Zachary Van Roy,

Gunjan Kak,

Rachel W. Fallet

et al.

Journal of Neuroinflammation, Journal Year: 2025, Volume and Issue: 22(1)

Published: Feb. 22, 2025

A craniotomy is a neurosurgical procedure performed to access the intracranial space. In 3–5% of cases, infections can develop, most caused by Staphylococcus aureus biofilm formation on skull surface. Medical management this infection difficult, as properties confer immune and antimicrobial recalcitrance necessitate additional surgical procedures. Furthermore, treatment failure rates be appreciably high. These factors, compounded with rapidly expanding resistance, highlight need develop alternative strategies target reverse dysfunction that occurs during infection. Our recent work has identified CD4+ Th1 Th17 cells potent regulators innate cell activation Here, we report role IFN-γ, versus other Th1- Th17-derived cytokines, in programing response using both global type-specific IFN-γR1-deficient (Ifngr1−/−) mice. Bacterial burdens were significantly higher Ifngr1−/− relative WT animals despite few changes abundance. Single-cell transcriptomics candidate explanations for phenotype alterations death pathways, activation, MHC-II expression, T responses reduced While caspase-1 PMNs macrophage/microglial expression regulated IFN-γ signaling, no phenotypes observed either granulocyte- or macrophage/microglia conditional knockout mice, suggestive redundancy. Instead, decreased Th1/Th17 ratio was corroborated elevated IL-17 levels correlated dysfunctional cell-innate communication. Further, less effective than promoting S. bactericidal activity microglia macrophages. Collectively, identifies key protective enhancing macrophage microglial antibacterial activity. Therefore, controlled programming may represent novel therapeutic strategy chronic infections.

Language: Английский

Citations

Fibroblast growth factor 5: a novel biomarker for familial hypercholesterolaemia DOI

Andrea Baragetti,

A. Alieva,

Liliana Grigore

et al.

European Heart Journal, Journal Year: 2025, Volume and Issue: unknown

Published: Jan. 22, 2025

Identification of individuals affected by familial hypercholesterolaemia (FH) is suboptimal when genetic tests are unavailable. Relying only on low-density lipoprotein cholesterol (LDL-C) challenging as it may not allow distinguishing with FH from hypercholesterolaemic (HC) the general population. The aim this study was to determine whether biomarkers associated cardiovascular disease and/or inflammation identify and distinguish them HC individuals. A panel 264 proteins in plasma measured machine learning used search for those that can individuals, either genetically proven (genFH) or clinically diagnosed (clinFH) control Both genFH clinFH had elevated levels fibroblast growth factor 5 (FGF-5) compared controls (mean area under curve [AUC] > .990 both, P < .001) AUC >.990, .001), even after matching LDL-C levels. An immunoenzymatic assay confirmed FGF-5 all cohorts analysed. This analysis suggests could be a biomarker discriminate living

Language: Английский

Citations

The Naples prognostic score as a new predictive index of severe abdominal aortic calcification: a population-based study DOI

Qiang Tan, Jian Zhang,

Y H Peng

et al.

Frontiers in Cardiovascular Medicine, Journal Year: 2025, Volume and Issue: 12

Published: Feb. 18, 2025

Abdominal aortic calcification (AAC) is related to inflammation and nutritional status. The Naples prognostic score (NPS) an innovative biological marker capable of reflecting systemic This research seeks investigate the correlation NPS with severe abdominal (SAAC). evaluated data obtained from National Health Nutrition Examination Survey (NHANES) conducted between 2013 2014. variables were filtered utilizing Least Absolute Shrinkage Selection Operator (LASSO) regression. Weighted logistic regression models employed examine association SAAC. predictive value for risk SAAC was assessed receiver operating characteristic (ROC) curve. A subgroup analysis assess strength reliability findings. encompassed 2,854 participants, among whom 303 (11.87%) exhibited outcomes multivariate weighted revealed that participants a 3-4 points positively correlated in comparison control group [odds ratio (OR) = 2.07, 95% confidence interval (95%CI): 1.17-3.67]. area under curve (AUC) predicting using 0.635. results indicated there no significant difference noted across various population subgroups. positive has been observed this research. study offers valuable insights into prevention diagnosis Future longitudinal studies are warranted confirm causative relationships role clinical decision-making

Language: Английский

Citations

Dynamic responses to rejection in the transplanted human heart revealed through spatial transcriptomics DOI

Kaushik Amancherla, Angela M. Taravella Oill, Xavier Bledsoe

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2025, Volume and Issue: unknown

Published: March 6, 2025

ABSTRACT Allograft rejection following solid-organ transplantation is a major cause of graft dysfunction and mortality. Current approaches to diagnosis rely on histology, which exhibits wide diagnostic variability lacks access molecular phenotypes that may stratify therapeutic response. Here, we leverage image-based spatial transcriptomics at sub-cellular resolution in longitudinal human cardiac biopsies characterize transcriptional heterogeneity 62 adult pediatric heart transplant (HT) recipients during histologically-diagnosed rejection. Across 28 cell types, identified significant differences abundance CD4 + CD8 T cells, fibroblasts, endothelial cells across different biological classes (cellular, mixed, antibody-mediated). We observed broad overlap cellular states histologic severity class within grades would qualify for immunomodulatory treatment. Individuals who had resolved after therapy distinct transcriptomic profile relative those with persistent rejection, including 216 genes 6 types along pathways inflammation, IL6-JAK-STAT3 signaling, IFNα/IFNγ response, TNFα signaling. Spatial also linked long-term prognostic outcomes post-HT. These results underscore importance subtyping immunologic immune-cardiac interactions HT are therapeutically relevant short- rejection-related outcomes.

Language: Английский

Citations

Tetrahydrocurcumin alleviates colorectal tumorigenesis by modulating the SPP1/CD44 axis and preventing M2 tumor-associated macrophage polarization DOI

Mengting Zhou, Rui Li,

Guiyun Lian

et al.

Phytomedicine, Journal Year: 2025, Volume and Issue: unknown, P. 156674 - 156674

Published: March 1, 2025

Language: Английский

Citations

Single-Cell RNA Sequencing Reveals the Immune Landscape of Granulomatous Mastitis DOI

Ruhong Zhou,

Xianguang Deng,

Ruan Hui

et al.

Inflammation, Journal Year: 2025, Volume and Issue: unknown

Published: May 8, 2025

Granulomatous mastitis (GM) is a form of non-lactational breast inflammation that closely associated with autoimmune processes, however its underlying pathogenesis remains elusive. In this study, we employed single-cell RNA sequencing (scRNA-seq) to conduct comparative analysis GM lesion tissues versus normal tissues, thereby unveiling the immune profile tissues. Our investigation centered on T and NK cells, macrophages, epithelial endothelial cells. Notably, observed substantial infiltration cells in accompanied by disorders, an elevation Th1 cell counts, enrichment toll-like receptor (TLR) pathway, upregulation various factors including interferon-γ (IFN-γ), C-C motif chemokine ligand 3 (CCL3), CCL4, (C-X-C motif) (CXCL) 13, CD69, signal transducer activator transcription 1 (STAT1), heat shock protein family A member 1A (HSPA1A). Furthermore, macrophage subpopulations exhibited transition pro-inflammatory phenotype, enriched for pathways such as IFN-α, interleukin-6/janus kinase/signal (IL-6/JAK/STAT3), tumor necrosis factor-α/nuclear factor-κB (TNF-α/NF-κB). Mammary luminal demonstrated impaired estrogenic yet displayed prolactin downstream signaling pathways, namely JAK/STAT mitogen-activated kinase (MAPK) pathways. Additionally, vascular were found recruit prominent angiogenic Cellular interaction unveiled intricate network interactions between mesenchymal This study provides comprehensive landscape granulomatous offers some potential therapeutic targets disease.

Language: Английский

Citations

Gas Bubbles from Biodegradable Magnesium Implants Convey Mechanical Cues and Promote Immune Cell Stimulation DOI

Heithem Ben Amara,

Jincy Philip,

Omar Omar

et al.

Advanced Science, Journal Year: 2025, Volume and Issue: unknown

Published: May 11, 2025

Abstract In ever‐increasing numbers, patients are treated with biodegradable magnesium implants. While gas bubbles frequently arise in soft tissue overlying implants, their biological implications remain uncertain. This study investigates how bubble accumulation and evolution across various lengths time scales influence adjacent cell behavior rats. Bubbles accumulate tissues around during initial postimplantation days, then fully resorb. Alterations geometry coincide of cells, many macrophage phenotypes, increased expression the mechanosensitive ion‐channel Piezo1. Using spatially resolved transcriptomics, strong proinflammatory pathway activation is revealed near marked proliferative marker secreted phosphoprotein 1 ( Spp1 ). Spatial transcriptomics also reveals enrichment cytoskeletal rearrangement genes, demonstrating that cells respond to mechanical cues from bubbles. Notably, both bubble–implant distance strongly cellular response. Over time, as located farther implant, regenerative processes decline, inflammation predominates. These findings suggest implant degradation create an intricate local response influencing healing through inflammatory pathways. underscores need for implants controlled release meticulous monitoring patients.

Language: Английский

Citations

Transcriptomic Profile of Early Antral Follicles: Predictive Somatic Gene Markers of Oocyte Maturation Outcome DOI

Alessia Peserico, Barbara Barboni, Chiara Camerano Spelta Rapini

et al.

Cells, Journal Year: 2025, Volume and Issue: 14(10), P. 704 - 704

Published: May 12, 2025

Early antral follicles (EAfs) offer oocyte potential in Assisted Reproductive Technology (ART), but most fail to mature under current vitro maturation (IVM) protocols. This study examines transcriptomic profiles of the follicular wall (FW) compartment during IVM ovine EAfs using a 3D follicle-enclosed (FEO) culture identify somatic gene markers predicting success. Differentially expressed genes (DEGs) were identified across three comparisons: pre- vs. post-hCG FW enclosing mature/fertilizable (1) or immature (2) oocytes, and between supporting successful failed (3). Network analysis highlighted key modulated HUB genes. Two DEG categories emerged: regulating meiosis resumption defining signatures linked competence. Meiosis involved ECM remodeling, hypoxia, relaxin signaling activation, while proliferative metabolic pathways downregulated. MMP13 EGFR regulated pathway, working for resumption, TGFB1 predicted failure. Oocyte competence involves activation suppression stress cell cycle pathways, with ITIH4 being conducive central tuning inflammation angiogenesis-dependent maturation. reveals molecular mechanisms behind follicle maturation, identifying releasing incompetent oocytes. It confirms known biomarkers uncovers new regulators, offering tools assess quality, improve IVF–oocyte selection, enhance fertility preservation.

Language: Английский

Citations

Role of macrophages in vascular calcification: From the perspective of homeostasis DOI

Rong Dong,

Zhenjun Ji, Mi Wang

et al.

International Immunopharmacology, Journal Year: 2024, Volume and Issue: 144, P. 113635 - 113635

Published: Nov. 19, 2024

Language: Английский

Citations

Multi-omics identification of extracellular components of the fetal monkey and human neocortex. DOI

Felipe Vilicich,

Dhanya Vettiatil,

Seth Kattapong-Graber

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2024, Volume and Issue: unknown

Published: Sept. 26, 2024

During development, precursor cells are continuously and intimately interacting with their extracellular environment, which guides ability to generate functional tissues organs. Much is known about the development of neocortex in mammals. This information has largely been derived from histological analyses, heterochronic cell transplants, genetic manipulations mice, a lesser extent transcriptomic analyses humans. However, these approaches have not led characterization composition developing any species. Here, using combination single-cell published datasets, our proteomics immunohistofluorescence we provide more comprehensive unbiased picture early fetal humans non-human primates. Our findings starting point for further hypothesis-driven studies on structural signaling components cortex that had previously identified.

Language: Английский

Citations