Utilizing coriander as a natural remedy for combating Eimeria papillata infection and oxidative stress in mice

Journal of Taibah University for Science, Journal Year: 2025, Volume and Issue: 19(1)

Published: Feb. 17, 2025

Language: Английский

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Fighting eimeriosis by using the anti-eimerial and anti-apoptotic properties of rhatany root extract

Frontiers in Immunology, Journal Year: 2024, Volume and Issue: 15

Published: July 11, 2024

Background Over the last decade, extensive use of coccidiostats to treat and control Eimeria infection has developed drug resistance, prompting search for new alternative therapies. Rhatany is proven have various pharmacological properties. Objective The present study aimed in vitro vivo evaluate effect roots extract (RRE) as an anti-eimerial anti-apoptotic agent against murine eimeriosis induced by papillata . Methods Phytochemical screening gas chromatography-mass spectrometry analysis (GC-MS) was used detect active compounds RRE. In activity RRE (200, 100, 50 mg/ml), amprolium, phenol, Dettol™, formalin were studied after incubation with non-sporulated oocysts. For study, twenty-five male C57BL/6 mice randomly allocated into five groups. Animals first group just given distilled H 2 O, while those second 200 mg/kg 5 days. parasite’s oocysts infected third, fourth, fifth treatment, (200 mg/kg) amprolium (120 orally 4 th groups days, respectively. All euthanized, on day post-infection, collect jejunal tissues under study. Investigations undertaken oocyst output feces goblet cells jejuna. Assays glutathione peroxidase (GPx), hydrogen peroxide (H O ), myeloperoxidase (MPO) also performed. tissue, cysteine aspartic acid protease-3 (Caspase-3) counted using immunohistochemistry, BCL2-associated X protein (Bax) B-cell lymphoma-2 (BCL-2) assayed ELISA. addition, mRNA expression cell response gene (MUC2) detected real-time PCR. Results GC-MS demonstrated presence 22 revealed that significantly inhabited sporulation a dose-dependent manner. By parasite, number reduced treatment (1.308 × 10 6 ± 1.36 oocysts/g feces) compared (5.387 4.29 feces). Moreover, jejuna its specific gene, MUC2. increased cells/villus from 3.45 0.17 6.04 0.23, associated upregulation MUC2 0.26 2.39-fold. Also, experimental lowered antioxidant enzyme represented GPx (23.99 3.68 mg/g tissue), increasing stress parameters (0.07 0.01 mM/g) well MPO (66.30 3.74 U/mg). production apoptotic markers including Caspase-3 (68.89 2.67 U/g) Bax (159.05 6.50 pg/ml) elevated decreasing marker BCL2 (0.42 0.07 pg/ml). Our proved oxidative stress, inflammatory MPO. jejunum E. -infected enhanced treatment. Conclusion highlights potential natural solution coccidiosis management modulating apoptosis host cells. However, further research needed fully understand underlying mechanisms enhance our understanding therapeutic efficacy.

Language: Английский

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Krameria lappacea roots extract to rescue coccidiosis-mediated inflammation in the jejunum of C57BL/6 mice

Frontiers in Immunology, Journal Year: 2025, Volume and Issue: 16

Published: April 17, 2025

Coccidiosis is a protozoan disease caused by Eimeria species, which multiply in the intestinal tract and lead to severe inflammatory responses. While coccidiostats are available for control, resistance these treatments has been confirmed, underscoring need new eco-friendly approaches. In recent years, natural plant sources have gained attention as effective alternatives treating various parasitic diseases. Krameria lappacea used traditional medicine due its pharmacological properties. This study examined effects of aqueous methanolic extract K. roots (KLRE) on jejunal inflammation immune response murine model infected with papillata. Twenty-five male C57BL/6 mice were randomly divided into five groups. The first group received only distilled water, while second was administered 200 mg/kg KLRE 5 days. third, fourth, fifth groups orally injected 103 sporulated oocysts parasite. For treatment, fourth (200 mg/kg), amprolium (120 mg/kg) All euthanized day post-infection (p.i.), blood samples jejunum collected. Investigations conducted assess oocyst shedding, cellular response, histological changes mice. Levels interleukin (IL)-1β, IL-6, tumor necrosis factor (TNF)-α measured using an enzyme-linked immunosorbent assay (ELISA). Additionally, mRNA expression CXC motif chemokine ligand 10 (CXCL10), interferon-inducible gene 202b (IFi202b), secreted phosphoprotein 1 (SPP-1) analyzed quantitative reverse transcription polymerase chain reaction (qRT-PCR). Our demonstrated that E. papillata produced average 5.387 × 106 ± 4.29 105 per gram feces post-infection. contrast, output significantly reduced 1.308 1.36 treated KLRE. These findings suggest host intracellular parasite triggers injury evidenced several factors: (i) elevated score, (ii) increased characterized neutrophils lymphocytes, (iii) protein levels IL-1β, TNF-α, at approximately 13.67 2.07, 78.98 4.17, 222.28 10.18 pg/ml, respectively, (iv) upregulated genes CXCL10, IFi202b, SPP-1, showed fold 2.83, 3.55, 3.07-fold, respectively. found all parameters associated infection altered during treatment data damage infections.

Language: Английский

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Cassia alata Extract Exerts Antioxidant Power to Mitigate Eimeria papillata-Induced Liver Damage in Mice

Indian Journal of Animal Research, Journal Year: 2024, Volume and Issue: Of

Published: Dec. 28, 2024

Background: This study was intended to investigate the prospective role of Cassia alata extract as an anticoccidial, anti-inflammatory and hepatoprotective agent against Eimeria papillata parasitic infection. Therefore, this potential Methods: For investigation, a total 25 male swiss albino mice were allocated into 5 equal groups. Group ∣: negative control (Cont), Π: administrated with (CE) at dosage 500 mg/kg body weight, ∃: infected group E. (Inf), IV: infected-treated (Inf-CE) weight V: amprolium 120 (Drug). Mice orally approximate 1×103 sporulated oocysts excluding ∣ Π One-hour post infection, groups IV V received recommended doses treatments once day for sequential days. Upon treatment days, all sacrificed growth performance, histopathological, histochemical oxidative stress assessments examined. Result: An enhancement in (-2.33±0.58%) feed intake (130.33±4.51 grams) relation (-4.67±0.58%) (98.00±4.36 Inf observed. Improvements liver structure alterations induced by recorded noticeable increase its carbohydrates (6.04±0.45%) protein (12.46±1.13%) content. Moreover, considerable reduction hepatic nitric oxide (59.13±4.59 Umol/g) malonaldehyde (22.07±1.20 nmol/mg) elevation glutathione (18.20±1.79 ng/mg) peroxidase (111.53±8.95 documented. In summary, CE could significantly ameliorate injury, inflammatory reactions caused

Language: Английский

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